{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:30:48Z","timestamp":1787027448819,"version":"build-2736575974"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:00:00Z","timestamp":1726185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Since boar spermatozoa show a marked deterioration in sperm quality when cooled, insemination doses are usually stored at 16\u201318 \u00b0C. However, maintaining this temperature during transport of semen doses is challenging, particularly during the summer months. An alternative could be to store the doses at 4 \u00b0C if cold-shock to the sperm could be prevented. The objective of this study was to evaluate boar sperm quality and fertility in\n                      <jats:italic>in vitro<\/jats:italic>\n                      fertilization after storage in AndroStar Premium at 4 \u00b0C for 1 week.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Insemination doses (\n                      <jats:italic>n<\/jats:italic>\n                      =\u20099) in AndroStar Premium from a commercial boar semen collection station were transported to the laboratory at approximately 20 \u00b0C. At the laboratory, sperm quality evaluation and was preformed and each dose was split; half of each ejaculate was stored in a climate-controlled box at 16\u201318 \u00b0C, the other was slowly cooled to 4 \u00b0C. Both samples were stored for 1 week before further sperm quality evaluation and\n                      <jats:italic>in vitro<\/jats:italic>\n                      fertilization (IVF) were performed. Mean values were tested using generalized linear regression, with treatment and boar as fixed factors;\n                      <jats:italic>p<\/jats:italic>\n                      \u2264\u20090.05 was considered significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Sperm membrane integrity (mean\u2009\u00b1\u2009sem: 91\u2009\u00b1\u20090.05 and 83\u2009\u00b1\u20090.09% for 16 and 4 \u00b0C, respectively) and superoxide production (6.79\u2009\u00b1\u20092.37 and 13.54\u2009\u00b1\u20096.23% for 16 and 4 \u00b0C, respectively), were different between treatments. The DNA fragmentation index was lower in cold-stored samples than in conventionally stored samples (3.74\u2009\u00b1\u20092.25 and 7.40\u2009\u00b1\u20093.36% for 4 and 16 \u00b0C, respectively). The numbers of oocytes developing to blastocyst on Day 6 (mean\u2009\u00b1\u2009sd: 9.0\u2009\u00b1\u20098.0 and 6.0\u2009\u00b1\u20095.0%, for storage at 16 and 4 \u00b0C, respectively) were not different between treatments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Therefore, storage of boar semen doses in AndroStar Premium at 4 \u00b0C for up to 7\u2009days would be a viable alternative to current praxis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2024.1444550","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T09:30:11Z","timestamp":1726219811000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Sperm quality and in vitro fertilizing ability of boar spermatozoa stored at 4 \u00b0C versus conventional storage for 1 week"],"prefix":"10.3389","volume":"11","author":[{"given":"Ida","family":"Hallberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane M.","family":"Morrell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pack","family":"Malaluang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Johannisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ylva","family":"Sjunnesson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Laskowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1796","DOI":"10.3390\/antibiotics11121796","article-title":"Bacteria and boar semen storage: progress and challenges","volume":"11","author":"Contreras","year":"2022","journal-title":"Antibiotics"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"83","DOI":"10.5772\/16592","article-title":"Artificial insemination in pigs. 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