{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:38:46Z","timestamp":1783953526169,"version":"3.55.0"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Genetic selection in broiler chickens has led to increased muscle mass without comparable respiratory and cardiovascular system development, limiting the birds\u2019 capacity to withstand high ambient temperatures and making them vulnerable to heat stress (HS). Early embryonic Thermal Manipulation (TM) has been suggested as an effective and sustainable way to mitigate the adverse effects of HS. This study investigated how these interventions influenced the immune status of broiler chickens exposed to HS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cobb 500 fertile eggs (<jats:italic>n<\/jats:italic>\u202f=\u202f600) were incubated according to guidelines. On embryonic day (ED) 12, the eggs were split into two groups: (1) Control, kept at standard temperature until hatch day (ED 21) and (2) Thermal Manipulation (TM), exposed to 38.5\u00b0C with 55% humidity for 12\u202fh daily from ED 12 to ED 18. After hatching, chicks were divided into (1) Control, (2) TM, (3) Control under Heat Stress (CHS), (4) TM under Heat Stress (TMHS), (5) Control with Heat Stress and Supplementation (CHSS), and (6) TM with Heat Stress and Supplementation (TMHSS). For the first 21\u202fdays, all chicks were raised under normal conditions. From day 22 to day 35, groups CHS, TMHS, CHSS, and TMHSS experienced chronic heat stress (32\u201333\u00b0C for 8\u202fh daily), while the Control and TM groups remained in a thermoneutral environment (22\u201324\u00b0C).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and discussion<\/jats:title><jats:p>TM significantly increased (<jats:italic>p<\/jats:italic>\u202f&amp;lt;\u202f0.05) <jats:italic>AvBD11, IL4,<\/jats:italic> and <jats:italic>TLR21<\/jats:italic> expression in the spleen. TM and baicalein supplementation significantly decreased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>TLR15<\/jats:italic> expression. In the bursa, TM significantly increased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>IL4<\/jats:italic> expression. The combination of TM with baicalein significantly increased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>CD3<\/jats:italic> and decreased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>TLR1<\/jats:italic> expression. Interestingly, TM alone significantly decreased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>IFNg<\/jats:italic> expression under HS condition. In the thymus, TM significantly decreased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>IL10<\/jats:italic> and <jats:italic>TLR15<\/jats:italic>, while incorporating baicalein with TM decreased (<jats:italic>p\u202f&amp;lt;<\/jats:italic> 0.05) <jats:italic>AvBD6<\/jats:italic> expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TM improved the immune status of broiler chickens under normal conditions. When combined with baicalein, TM mitigated the negative effects of heat stress by boosting key immune-related gene expression in the spleen, bursa, and thymus.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fvets.2025.1537116","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T06:43:40Z","timestamp":1738046620000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Early embryonic thermal programming and post-hatch flavonoid (Scutellaria baicalensis) supplementation enhanced immune response markers in broiler chickens"],"prefix":"10.3389","volume":"12","author":[{"given":"Sadid","family":"Al Amaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Ahosanul Haque","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Jha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birendra","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"ref1","volume-title":"Climate change 2023: synthesis report. 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