{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T20:08:29Z","timestamp":1780085309705,"version":"3.54.0"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Brucellosis, caused by <jats:italic>Brucella<\/jats:italic>, is a severe zoonotic disease. Conventional IgG antibody-based ELISA testing faces challenges such as false positives and cross-reactivity. In this study, three specific chicken yolk antibodies (IgY) targeting <jats:italic>Brucella<\/jats:italic> were isolated from the eggs of immunized hens, and a method for detecting <jats:italic>Brucella<\/jats:italic> utilizing these antibodies was developed and subsequently compared to traditional IgG antibodies. These IgY antibodies were generated against a fusion protein, LPS, and whole-cell antigen, and their potency was evaluated through indirect ELISAs. Testing was conducted to assess cross-reactivity, limit of detection, and detection in simulated samples. The IgY antibodies demonstrated high potency and no cross-reactivity with common foodborne pathogens. Both LPS-IgY and <jats:italic>Brucella<\/jats:italic>-IgY showed excellent detection capabilities in identifying <jats:italic>Brucella<\/jats:italic>, particularly in food samples. These results underscore the potential of using LPS-IgY and <jats:italic>Brucella<\/jats:italic>-IgY antibodies as replacements for conventional IgG antibodies in <jats:italic>Brucella<\/jats:italic> detection, especially in the realm of food safety. The implications of this study are significant, as it presents a promising alternative approach for detecting <jats:italic>Brucella<\/jats:italic> in food products, thereby reducing the risk of transmission and ensuring public health and safety.<\/jats:p>","DOI":"10.3389\/fvets.2025.1552097","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T08:44:34Z","timestamp":1747039474000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Preparation and application of specific chicken yolk antibodies in detecting Brucella"],"prefix":"10.3389","volume":"12","author":[{"given":"Xinru","family":"Qi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianhan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qichuan","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dehui","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiansong","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s12889-017-5016-y","article-title":"Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action","volume":"18","author":"Franc","year":"2018","journal-title":"BMC Public Health."},{"key":"B2","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1002\/vms3.264","article-title":"Detection of Brucella antibodies in domestic animals of southern Cameroon: implications for the control of brucellosis","volume":"6","author":"Kamga","year":"2020","journal-title":"Vet Med Sci."},{"key":"B3","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1186\/s40249-020-00715-1","article-title":"Human brucellosis: recent advances and future challenges","volume":"9","author":"O'Callaghan","year":"2020","journal-title":"Infect Dis Poverty."},{"key":"B4","doi-asserted-by":"publisher","first-page":"164948","DOI":"10.1016\/j.scitotenv.2023.164948","article-title":"Synergistic driving effects of risk factors on human brucellosis in Datong City, China: a dynamic perspective from spatial heterogeneity","volume":"894","author":"Shen","year":"2023","journal-title":"Sci Total Environ."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.3201\/eid2909.230052","article-title":"Global estimate of human brucellosis incidence","volume":"29","author":"Laine","year":"2023","journal-title":"Emerg Infect Dis."},{"key":"B6","doi-asserted-by":"publisher","first-page":"S31","DOI":"10.1016\/j.ijvsm.2018.01.008","article-title":"Brucellosis: evolution and expected comeback","volume":"6","author":"El-Sayed","year":"2018","journal-title":"Int J Vet Sci Med."},{"key":"B7","unstructured":""},{"key":"B8","doi-asserted-by":"publisher","first-page":"900","DOI":"10.2460\/javma.233.6.900","article-title":"Brucellosis","volume":"233","author":"Glynn","year":"2008","journal-title":"J Am Vet Med Assoc."},{"key":"B9","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1177\/014107680509801009","article-title":"How Themistocles Zammit found Malta Fever (brucellosis) to be transmitted by the milk of goats","volume":"98","author":"Wyatt","year":"2005","journal-title":"J R Soc Med."},{"key":"B10","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.tmaid.2018.02.005","article-title":"Clinically-diagnosed Mediterranean Spotted Fever in Malta","volume":"26","author":"Torpiano","year":"2018","journal-title":"Travel Med Infect Dis."},{"key":"B11","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.ijid.2019.09.013","article-title":"Monitoring the course of Brucella infection with qPCR-based detection","volume":"89","author":"Che LH Qi","year":"2019","journal-title":"Int J Infect Dis."},{"key":"B12","doi-asserted-by":"publisher","first-page":"758564","DOI":"10.3389\/fbioe.2021.758564","article-title":"A label-based polymer nanoparticles biosensor combined with loop-mediated isothermal amplification for rapid, sensitive, and highly specific identification of Brucella abortus","volume":"9","author":"Yang","year":"2021","journal-title":"Front Bioeng Biotechnol"},{"key":"B13","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.prevetmed.2018.01.005","article-title":"A systematic review of current immunological tests for the diagnosis of cattle brucellosis","volume":"151","author":"Ducrotoy","year":"2018","journal-title":"Prev Vet Med"},{"key":"B14","doi-asserted-by":"publisher","first-page":"29534","DOI":"10.1021\/acsomega.3c03381","article-title":"Novel vertical flow immunoassay with Au@PtNPs for rapid, ultrasensitive, and on-site diagnosis of human Brucellosis","volume":"8","author":"Lu","year":"2023","journal-title":"ACS Omega"},{"key":"B15","doi-asserted-by":"publisher","first-page":"696003","DOI":"10.3389\/fimmu.2021.696003","article-title":"Immunoglobulin Y for potential diagnostic and therapeutic applications in infectious diseases","volume":"12","author":"Lee","year":"2021","journal-title":"Front Immunol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.jgeb.2017.10.003","article-title":"Production and purification of IgY antibodies from chicken egg yolk","volume":"16","author":"Amro","year":"2018","journal-title":"J Genet Eng Biotechnol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"708008","DOI":"10.3389\/fvets.2021.708008","article-title":"A multi-epitope fusion protein-based p-ELISA method for diagnosing bovine and goat brucellosis","volume":"8","author":"Yin","year":"2021","journal-title":"Front Vet Sci"},{"key":"B18","doi-asserted-by":"publisher","first-page":"3084","DOI":"10.3791\/3084","article-title":"IgY technology: extraction of chicken antibodies from egg yolk by polyethylene glycol (PEG) precipitation","volume":"51","author":"Pauly","year":"2011","journal-title":"J Vis Exp."},{"key":"B19","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1186\/s12879-018-3134-5","article-title":"Brucella abortus: determination of survival times and evaluation of methods for detection in several matrices","volume":"18","author":"Kaden","year":"2018","journal-title":"BMC Infect Dis."},{"key":"B20","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.ab.2017.11.010","article-title":"Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection","volume":"542","author":"Pang","year":"2018","journal-title":"Anal Biochem."},{"key":"B21","doi-asserted-by":"publisher","first-page":"62","DOI":"10.3390\/antib11040062","article-title":"IgY antibodies as biotherapeutics in biomedicine","volume":"11","author":"Le\u00f3n-N\u00fa\u00f1ez","year":"2022","journal-title":"Antibodies."},{"key":"B22","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.jim.2018.06.023","article-title":"Production and application of anti-nucleoprotein IgY antibodies for influenza A virus detection in swine","volume":"461","author":"da Silva","year":"2018","journal-title":"J Immunol Methods."},{"key":"B23","doi-asserted-by":"publisher","first-page":"227","DOI":"10.2217\/bmm.10.7","article-title":"Use of IgY antibodies and semiconductor nanocrystal detection in cancer biomarker quantitation","volume":"4","author":"Xiao","year":"2010","journal-title":"Biomark Med."},{"key":"B24","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/0147-9571(90)90088-b","article-title":"Chicken IgY: utilizing the evolutionary difference","volume":"13","author":"Larsson","year":"1990","journal-title":"Comp Immunol Microbiol Infect Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1253349","DOI":"10.3389\/fmicb.2023.1253349","article-title":"Immuno-profiling of Brucella proteins for developing improved vaccines and DIVA capable serodiagnostic assays for brucellosis","volume":"14","author":"Nandini","year":"2023","journal-title":"Front Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"5362","DOI":"10.1039\/d1an01075a","article-title":"Flower-like gold nanoparticles labeled and silver deposition rapid vertical flow technology for highly sensitive detection of Brucella antibodies","volume":"146","author":"Fang","year":"2021","journal-title":"Analyst."},{"key":"B27","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.vetimm.2009.12.011","article-title":"IgY: a promising antibody for use in immunodiagnostic and in immunotherapy","volume":"135","author":"Dias","year":"2010","journal-title":"Vet Immunol Immunopathol"},{"key":"B28","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1177\/026119290503300208","article-title":"Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine","volume":"33","author":"Schade","year":"2005","journal-title":"Altern Lab Anim."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2025.1552097\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T08:44:36Z","timestamp":1747039476000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2025.1552097\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,12]]},"references-count":28,"alternative-id":["10.3389\/fvets.2025.1552097"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2025.1552097","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,12]]},"article-number":"1552097"}}