{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T18:30:50Z","timestamp":1793557850449,"version":"4.5.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Pneumonic pasteurellosis is a highly significant infectious disease globally, posing a major economic threat to small ruminants (SR) in Sub-Saharan Africa, including Ethiopia. Although antibiotics have been effective in treating this disease, farmers in remote areas of Ethiopia often prefer traditional herbal medicines to manage respiratory infections in SR. This study aimed to evaluate the antibacterial activity of crude extracts from three selected medicinal plants\u2014\n                    <jats:italic>Nicotiana tabacum, Psidium guajava<\/jats:italic>\n                    , and\n                    <jats:italic>Solanum incanum\u2014<\/jats:italic>\n                    against respiratory infections caused by\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    and\n                    <jats:italic>Mannheimia haemolytica<\/jats:italic>\n                    in SR, in comparison to commercial gentamicin, oxytetracycline, and streptomycin antibiotics. The Agar well diffusion method was used to determine the antibacterial activity of methanol and chloroform extracts from the three medicinal plants against\n                    <jats:italic>P. multocida<\/jats:italic>\n                    and\n                    <jats:italic>M. haemolytica<\/jats:italic>\n                    strains. The phytochemical constituents of the plant extracts were analyzed by using standard phytochemical screening methods. Methanol extracts from all three plants demonstrated significant antibacterial activity against both bacterial strains (\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    and\n                    <jats:italic>Mannheimia haemolytica<\/jats:italic>\n                    ) at a concentration of 200\u202fmg\/mL, comparable to the effectiveness of gentamicin and streptomycin. Among the three plants,\n                    <jats:italic>S. incanum<\/jats:italic>\n                    showed the highest zone of inhibition (26.3\u202fmm), followed by\n                    <jats:italic>N. tabacum<\/jats:italic>\n                    (19.8\u202fmm) and\n                    <jats:italic>P. guajava<\/jats:italic>\n                    (19.6\u202fmm) against the tested strains. Similarly, chloroform extracts also exhibited notable activity, with\n                    <jats:italic>P. guajava<\/jats:italic>\n                    showing the highest inhibition (30.2\u202fmm) against\n                    <jats:italic>P. multocida<\/jats:italic>\n                    at 200\u202fmg\/mL. Phytochemical screening revealed the presence of various bioactive compounds, including alkaloids, flavonoids, tannins, saponins, and terpenoids. These findings support the traditional use of these medicinal plants in treating respiratory infections caused by\n                    <jats:italic>P. multocida<\/jats:italic>\n                    and\n                    <jats:italic>M. haemolytica<\/jats:italic>\n                    in SR.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2025.1563208","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T01:22:29Z","timestamp":1748827349000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluating antimicrobial activity of selected medicinal plant extracts against pasteurellosis-causing bacteria in small ruminants"],"prefix":"10.3389","volume":"12","author":[{"given":"Beshada","family":"Asfa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dereje Nigussie","family":"Woldemichael","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuwork","family":"Tesfaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lishan","family":"Asefa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solomon","family":"Desta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sisay","family":"Girma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teshale Sori","family":"Tolera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takele Beyene","family":"Tufa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"22","DOI":"10.5897\/jcab2016.0449","article-title":"Sheep and goats pasteurellosis: isolation, identification, biochemical characterization and prevalence determination in Fogera Woreda, Ethiopia","volume":"10","author":"Tewodros","year":"2016","journal-title":"J Cell Anim Biol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/09712119.2022.2146123","article-title":"A review on pneumonic pasteurellosis in small ruminants","volume":"51","author":"Abera","year":"2022","journal-title":"J Appl Anim Res"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.cvfa.2010.10.016","article-title":"Treatment and control of respiratory disease in 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