{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:03:49Z","timestamp":1790816629140,"version":"4.1.0"},"reference-count":105,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities of China","doi-asserted-by":"publisher","award":["31920240104"],"award-info":[{"award-number":["31920240104"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities of China","doi-asserted-by":"publisher","award":["31920240088"],"award-info":[{"award-number":["31920240088"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Deoxynivalenol (DON) and Zearalenone (ZEN), common symbiotic mycotoxins found in mold-contaminated cereal feed, adversely affect broiler\u2019 health. <jats:italic>Glycyrrhiza uralensis<\/jats:italic> has various pharmacological effects including antibacterial, antioxidant and immunomodulatory. This study aimed to investigate the effects of the long-term intake of low doses of DON and ZEN on growth performance and intestinal health of broilers, as well as the potential protective effect of supplementary <jats:italic>Glycyrrhiza uralensis<\/jats:italic> extract (GUE) in an 84-day feeding experiment. A total of 315 one-day-old male <jats:italic>Liangfeng<\/jats:italic> broilers were randomly assigned to three treatments: basal diet (CON), MOL diet (where 5% of corn in the basal diet was replaced with an equal amount of naturally moldy corn) containing DON and ZEN at 1.25 and 1.29 mg\/kg, and MGUE diet supplemented with 0.1% GUE in the MOL diet. The MOL diet reduced the body weight (BW) of broilers at 56 and 84 day, body weight gain (BWG) and feed intake (FI) aged 1-56 and 1-84 days, and the feed conversion ratio (FCR) aged 1-84 days, as well as villus height (VH) and the villus\/crypt (V\/C) ratio, SOD and GSH-Px activities, and the expression of <jats:italic>claudin-1<\/jats:italic>, <jats:italic>occludin<\/jats:italic> and <jats:italic>ZO-1<\/jats:italic>, while increasing MDA level, the expression of <jats:italic>TNF-\u03b1<\/jats:italic>, <jats:italic>IL-1\u03b2<\/jats:italic> and <jats:italic>IFN-\u03b3<\/jats:italic> in the jejunum of broilers. Additionally, MOL diet decreased the <jats:italic>Firmicutes<\/jats:italic> to <jats:italic>Bacteroidetes<\/jats:italic> (F\/B) ratio and abundances of <jats:italic>Lactobacillus<\/jats:italic> (<jats:italic>L.gallinarum<\/jats:italic> and <jats:italic>L.crispatus<\/jats:italic>), and <jats:italic>B.vulgatus<\/jats:italic>, while increasing <jats:italic>Bacteroides<\/jats:italic> (<jats:italic>B.fragilis<\/jats:italic> and <jats:italic>B.dore<\/jats:italic>), <jats:italic>Helicobacter<\/jats:italic> (<jats:italic>H.pullorum<\/jats:italic>), and <jats:italic>Escherichia<\/jats:italic> (<jats:italic>E.coli<\/jats:italic>) in the ceca. In contrast, MGUE diet improved growth performance and returned it to a level comparable to that of the CON diet, increased VH and V\/C ratio, SOD and GSH-Px activity, <jats:italic>claudin-1<\/jats:italic>, <jats:italic>occludin<\/jats:italic> and <jats:italic>ZO-1<\/jats:italic> expression, while reducing MDA level, the expression of <jats:italic>TNF-\u03b1<\/jats:italic>, <jats:italic>IL-1\u03b2<\/jats:italic> and <jats:italic>IFN-\u03b3<\/jats:italic> in the jejunum. Moreover, MGUE diet had a greater F\/B ratio and abundance of <jats:italic>Lactobacillus<\/jats:italic> (<jats:italic>L.gallinarum<\/jats:italic> and <jats:italic>L.crispatus<\/jats:italic>) and <jats:italic>B.vulgatus<\/jats:italic>, while reducing <jats:italic>Bacteroides<\/jats:italic> (<jats:italic>B.fragilis<\/jats:italic> and <jats:italic>B.dorei<\/jats:italic>), <jats:italic>Helicobacter<\/jats:italic> (<jats:italic>H.pullorum<\/jats:italic>) and <jats:italic>Escherichia<\/jats:italic> (<jats:italic>E.coli<\/jats:italic>) in cecum. In conclusion, the long-term consumption of a low-dose DON-ZEN contaminated diet decreases growth performance and disrupts intestinal health and microbiota balance in broilers; however, dietary supplementation with GUE effectively mitigates the damage caused by DON-ZEN contamination.<\/jats:p>","DOI":"10.3389\/fvets.2025.1570265","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T15:14:06Z","timestamp":1744384446000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Glycyrrhiza uralensis extract supplementation mitigated the negative effects of prolonged low-dose exposure to Deoxynivalenol and Zearalenone on growth performance and intestinal health of broiler chickens"],"prefix":"10.3389","volume":"12","author":[{"given":"Yan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingpai","family":"Zha Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxiong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1017\/s1751731111001601","article-title":"Fusarium mycotoxin-contaminated wheat containing Deoxynivalenol alters the gene expression in the liver and the jejunum of broilers","volume":"6","author":"Dietrich","year":"2012","journal-title":"Animal"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1042\/ebc20220221","article-title":"Diverse mycotoxin threats to safe food and feed cereals","volume":"67","author":"Jones","year":"2023","journal-title":"Essays 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