{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:36:17Z","timestamp":1753893377716,"version":"3.41.2"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>This report aims to present a case of obstructive azoospermia in a stud stallion diagnosed with an alkaline phosphatase (SPAP) assessment. A 20-year-old Murgese stallion is referred for acquired azoospermia. History is negative for reproductive disorders, and clinical examinations and ultrasonography of internal and external genitalia do not reveal significant alterations. Semen collection highlights the absence of spermatozoa in the ejaculate and the urinalysis is negative for spermatozoa. SPAP assay is performed on seminal plasma, with a value of 30\u202fIU\/L, compatible with obstructive azoospermia. A biopsy is performed, detecting the presence of complete germ lines in both testes. A resolution is attempted endoscopically, gently insufflating ampullae, with negative results, so the stallion is excluded from breeding. Time afterwards, the stallion dies of natural causes, and necropsy and histopathological analyses are performed. Corpora amylacea are highlighted in both seminal vesicles; the right and left ampullae show ectasic lumen, with the diffuse presence of hyaline material. Ampullae obstruction is an uncommon pathology, which can affect stallions and jacks, generally caused by the accumulation of spermatozoa, but, unfortunately, this case was unresponsive to attempted treatments. Interestingly, to the authors\u2019 knowledge, this is the first report of corpora amylacea in equine stallion sexual glands.<\/jats:p>","DOI":"10.3389\/fvets.2025.1571637","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T05:15:19Z","timestamp":1744348519000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Case Report: Reproductive evaluation of a Murgese stallion with obstructive azoospermia, accumulation of hyaline material in the ampullae ducts, and corpora amylacea in vesicular glands"],"prefix":"10.3389","volume":"12","author":[{"given":"Roberta","family":"Bucci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Parrillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Probo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Varasano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Romano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Augusto","family":"Carluccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"ref1","first-page":"336","article-title":"Clinical fertility evaluation of the stallion","author":"Kenney","year":"1976","journal-title":"Ann Meet Am Assoc Equine Practiti"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.theriogenology.2019.09.035","article-title":"Evaluation of the effect of laboratory methods on semen analysis and breeding soundness examination (BSE) classification in stallions","volume":"142","author":"Whitesell","year":"2020","journal-title":"Theriogenology"},{"key":"ref3","article-title":"Guidelines for using the canine breeding soundness evaluation form","author":"Purswell","year":"1992","journal-title":"Theriogenol Handbook"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"S148","DOI":"10.1017\/S1751731118000939","article-title":"Abnormalities of the bull\u2013occurrence, diagnosis, and treatment of abnormalities of the bull, including structural soundness","volume":"12","author":"Wolfe","year":"2018","journal-title":"Animal"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1177\/1098612X221079707","article-title":"Normal feline reproduction: the tom","volume":"24","author":"Johnson","year":"2022","journal-title":"J Feline Med Surg"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1136\/inp.b5503","article-title":"Prebreeding examination of the stallion: 1","volume":"32","author":"Crabtree","year":"2010","journal-title":"Phys Examination Pract"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/S0749-0739(17)30472-8","article-title":"Evaluation of the stallion for breeding soundness","volume":"8","author":"Hurtgen","year":"1992","journal-title":"Vet Clin N Am Equine Pract"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"S37","DOI":"10.1016\/j.jevs.2016.06.075","article-title":"Approaches to breeding soundness examination and interpretation of results","volume":"43","author":"Varner","year":"2016","journal-title":"J Equine Vet Sci"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1016\/j.jevs.2008.10.003","article-title":"Diagnostic methods for evaluation of stallion subfertility: a review","volume":"28","author":"Ball","year":"2008","journal-title":"J Equine Vet Sci"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/S0378-4320(00)00115-9","article-title":"Techniques for evaluating selected reproductive disorders of stallions","author":"Varner","year":"2000","journal-title":"Anim Reprod Sci"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/S0749-0739(17)30466-2","article-title":"Ejaculation: physiology and dysfunction","volume":"8","author":"McDonnell","year":"1992","journal-title":"Vet Clin N Am Equine Pract"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"331","DOI":"10.5555\/20103149546","article-title":"How to investigate azoospermia in stallions","volume":"55","author":"Blanchard","year":"2009","journal-title":"Proc. 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