{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:46:25Z","timestamp":1790390785383,"version":"4.1.0"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP25KJ2102"],"award-info":[{"award-number":["JP25KJ2102"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      International guidelines recommend the use of antiseptics, such as chlorhexidine, to treat canine pyoderma. However, data on the antiseptic susceptibility of its primary causative agents,\n                      <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                      and\n                      <jats:italic>S. coagulans<\/jats:italic>\n                      , in Japan are limited.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed antiseptic susceptibility testing and polymerase chain reaction (PCR) screening for antiseptic resistance-associated genes in these species. In addition, hybrid genome sequencing was conducted for a resistant isolate to investigate the genetic context of resistance genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Most isolates exhibited low minimum inhibitory concentrations for the tested antiseptics, although some inter-strain variations were observed. One\n                      <jats:italic>S. coagulans<\/jats:italic>\n                      isolate (SC18) was identified as smr-positive, representing only the second global report and the first from Japan. Phylogenetic analysis using publicly available genome data revealed that SC18 belongs to the major lineage of\n                      <jats:italic>S. coagulans<\/jats:italic>\n                      . Hybrid genome sequencing further demonstrated, for the first time, that\n                      <jats:italic>smr<\/jats:italic>\n                      in\n                      <jats:italic>S. coagulans<\/jats:italic>\n                      is plasmid-borne. Notably, this plasmid was also identified in a human-derived\n                      <jats:italic>S. epidermidis<\/jats:italic>\n                      strain (KSE124-2) in Japan, suggesting plasmid-mediated interspecies transmission between humans and companion animals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings highlight the need for continued surveillance of antiseptic resistance-associated genes, which may contribute to reduced phenotypic susceptibility and pose a potential public health concern.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2025.1621915","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T04:13:05Z","timestamp":1757304785000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Antiseptic susceptibility profiles of canine pyoderma-associated staphylococci in Japan: first identification of plasmid-borne smr in Staphylococcus coagulans"],"prefix":"10.3389","volume":"12","author":[{"given":"Manami","family":"Tsunoi","sequence":"first","affiliation":[{"name":"Core Research Facilities, Research Center for Medical Sciences, The Jikei University School of Medicine","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Takiguchi","sequence":"additional","affiliation":[{"name":"Laboratory Service for Animals, 1sec Co., Ltd","place":["Fujisawa, Kanagawa, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Ashida","sequence":"additional","affiliation":[{"name":"Laboratory Service for Animals, 1sec Co., Ltd","place":["Fujisawa, Kanagawa, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Harada","sequence":"additional","affiliation":[{"name":"Joint Graduate School of Veterinary Sciences, Tottori University","place":["Tottori, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Iyori","sequence":"additional","affiliation":[{"name":"Laboratory Service for Animals, 1sec Co., Ltd","place":["Fujisawa, Kanagawa, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Applied Mathematics, Tokyo University of Science","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e0014622","DOI":"10.1128\/cmr.00146-22","article-title":"Companions in antimicrobial resistance: examining transmission of common antimicrobial-resistant organisms between people and their dogs, cats, and horses","volume":"38","author":"Caddey","year":"2025","journal-title":"Clin 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