{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T16:22:51Z","timestamp":1763050971175,"version":"3.45.0"},"reference-count":99,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Biological invasions represent a significant epidemiological route for the introduction and dispersion of pathogens, facilitating disease emergence and transmission among native biodiversity. In the temperate rainforest ecoregion of south-central Chile, the native semiaquatic mustelid\n                      <jats:italic>Lontra felina<\/jats:italic>\n                      (marine otter) and\n                      <jats:italic>L. provocax<\/jats:italic>\n                      (southern river otter) coexist both sympatrically and syntopically with two invasive species\u2014American mink (\n                      <jats:italic>Neogale vison<\/jats:italic>\n                      ) and domestic dog (\n                      <jats:italic>Canis lupus familiaris<\/jats:italic>\n                      ), that act as carriers and hosts of canine parvovirus and distemper.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>To assess the occurrence of both diseases, we: (1) collected serum and mucous membrane samples from four species across three sectors of this ecoregion; and (2) employed serological immunoassays (IgG) and genetic analyses (qPCR-HRM) to detect both active and past infections, and to genotypically characterize the two viral agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      75% of\n                      <jats:italic>L. felina<\/jats:italic>\n                      individuals tested positive for parvovirus. The melting temperature (T\n                      <jats:sub>m<\/jats:sub>\n                      ) of the analyzed DNA fragment revealed two diverging groups, suggesting the presence of two genotypic variants of the virus within this mammalian assemblage.\n                      <jats:italic>L. felina<\/jats:italic>\n                      individuals carried the variant with the higher T\n                      <jats:sub>m<\/jats:sub>\n                      , which was also detected in\n                      <jats:italic>N. vison<\/jats:italic>\n                      from the same locality. In contrast,\n                      <jats:italic>L. provocax<\/jats:italic>\n                      individuals carried the variant with the lower T\n                      <jats:sub>m<\/jats:sub>\n                      , while dogs and minks hosted both viral variants. Canine distemper virus was detected only in dogs that also tested positive for parvovirus.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Our results present the first report of parvovirus in\n                      <jats:italic>L. felina<\/jats:italic>\n                      and support the hypothesis that\n                      <jats:italic>N. vison<\/jats:italic>\n                      and dogs acts as metareservoir and mink also as a bridge host for its transmission. In the study area, the synanthropic behavior of\n                      <jats:italic>N. vison<\/jats:italic>\n                      and its interactions with domestic and native species may facilitate the diversification of emergent pathogens within Chilean native fauna.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2025.1634282","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T16:17:50Z","timestamp":1763050670000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Tag, you\u2019re it!: viral diseases in native otters of south-central Chile due to coexistence with invasive American mink and domestic dogs"],"prefix":"10.3389","volume":"12","author":[{"given":"Alexis","family":"Santiba\u00f1ez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Coccia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erwin 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