{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T12:42:54Z","timestamp":1778503374122,"version":"3.51.4"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Bovine viral diarrhea virus (BVDV), a highly mutable pathogen, poses a significant threat to the cattle industry in China. Therefore, the development of a rapid, sensitive, and specific diagnostic assay is essential for effective surveillance and control. In this study, a TaqMan real-time quantitative PCR (qPCR) assay utilizing a minor groove binder (MGB) probe was developed for the detection of BVDV, with a focus on strains currently circulating in China. Universal primers and an MGB probe targeting the conserved 5\u2032 untranslated region (5\u2032UTR) of both BVDV-1 and BVDV-2 were designed based on complete genome sequences available in GenBank. Following optimization of the reaction conditions, the assay demonstrated a detection limit of 1.265 copies\/\u03bcL using a plasmid standard. The method exhibited high specificity for BVDV-1 and BVDV-2, with no cross reactivity observed with other common bovine pathogens. Intra- and inter-assay coefficients of variation were below 1.5%, indicating excellent repeatability and reproducibility. When applied to field serum samples collected from free-range cattle in various regions of China, the assay achieved a 100% concordance rate with a commercial reference kit (IDEXX RealPCR\u2122 BVDV RNA Test). These results suggest that the established TaqMan MGB qPCR assay is a reliable and efficient tool for the detection and epidemiological investigation of BVDV-1 and BVDV-2 infections in cattle herds across China.<\/jats:p>","DOI":"10.3389\/fvets.2025.1634429","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T09:50:10Z","timestamp":1753437010000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of a TaqMan qPCR method with MGB probe for the specific detection of BVDV field strains circulating in China"],"prefix":"10.3389","volume":"12","author":[{"given":"Lele","family":"An","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoting","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yetao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shubin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/bs.aivir.2017.07.003","article-title":"New leaves in the growing tree of Pestiviruses","volume":"99","author":"Blome","year":"2017","journal-title":"Adv Virus 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