{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T18:11:18Z","timestamp":1775758278299,"version":"3.50.1"},"reference-count":78,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>This study investigated the influence of dietary aspirin eugenol ester (AEE) on growth performance, ileum antioxidant capacity, intestinal barrier function and ileum microbiota of broilers subjected to high stocking density stress (HDS). A total of 360 one-day-old Arbor Acres broilers were randomly assigned to four treatment groups: normal density (ND, 14 broilers\/m<jats:sup>2<\/jats:sup>), normal density + AEE (NDAEE), high density (HD, 22 broilers\/m<jats:sup>2<\/jats:sup>) and high density + AEE (HDAEE). HDS leads to a significant decrease in broiler performance while reducing the antioxidant capacity of the gut. Morphologic examination of the intestine revealed that HDS causes damage to the intestinal villi. Dietary addition of AEE significantly increased body weight gain and improved antioxidant capacity with restoration of intestinal morphology in broilers with HDS. In addition, HDS resulted in decreased gene expression of ileum tight junction proteins and significantly increased gene expression of inflammatory factors in broilers. Dietary addition of AEE effectively alleviated the decrease in tight junction protein gene expression in broilers with HDS and reduced the expression of HDS-induced inflammatory cytokines through the <jats:italic>COX-2-mPGES-1<\/jats:italic> signaling pathway. AEE supplementation improved microbiota diversity and increased the relative abundance of <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>Ileibacterium<\/jats:italic>. Thus, dietary AEE effectively reduced the negative effects of HDS on productivity, gut microbiota and overall health in broilers and could be a worthwhile dietary supplement for offsetting negative effects of stocking stress in broilers.<\/jats:p>","DOI":"10.3389\/fvets.2025.1638245","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T04:10:35Z","timestamp":1751861435000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of dietary supplementation with aspirin eugenol ester on performance and ileum health in broilers under high stocking density stress conditions"],"prefix":"10.3389","volume":"12","author":[{"given":"Penghui","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongying","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenrui","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caifang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingkun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanbo","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"ref1","year":"2024"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"e07788","DOI":"10.2903\/j.efsa.2023.7788","article-title":"Welfare of broilers on farm","volume":"21","author":"Nielsen","year":"2023","journal-title":"EFSA J"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.3382\/ps.2009-00007","article-title":"Stocking density effects on broiler welfare: identifying sensitive ranges for different indicators","volume":"88","author":"Buijs","year":"2009","journal-title":"Poult Sci"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1844","DOI":"10.3382\/ps.2010-01277","article-title":"Economic impact of decreasing stocking densities in broiler production in Belgium","volume":"90","author":"Verspecht","year":"2011","journal-title":"Poult Sci"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1406","DOI":"10.3382\/ps.2010-01230","article-title":"Stocking density affects the growth performance of broilers in a sex-dependent fashion","volume":"90","author":"Zuowei","year":"2011","journal-title":"Poult Sci"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1080\/10888705.2021.2021653","article-title":"The effect of borage (Borago officinalis) extract on growth performance and immune system of broilers at high-density conditions","volume":"27","author":"Salehifar","year":"2022","journal-title":"J Appl Anim Welf Sci"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1322","DOI":"10.1080\/10495398.2021.1898413","article-title":"Effect of stocking density on performance, meat quality and cecal bacterial communities of yellow feather broilers","volume":"33","author":"Wang","year":"2021","journal-title":"Anim Biotechnol"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"642","DOI":"10.5455\/javar.2021.h556","article-title":"Impacts of stocking density rates on welfare, growth, and hemato-biochemical profile in broiler chickens","volume":"8","author":"Zabir","year":"2021","journal-title":"J Adv Vet Anim Res"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"102944","DOI":"10.1016\/j.jtherbio.2021.102944","article-title":"Thermal stress and high stocking densities in poultry farms: potential effects and mitigation strategies","volume":"99","author":"Bilal","year":"2021","journal-title":"J Therm Biol"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"2208","DOI":"10.3382\/ps\/pew137","article-title":"Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio","volume":"95","author":"Scanes","year":"2016","journal-title":"Poult Sci"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1878","DOI":"10.3382\/ps.2010-01311","article-title":"Effects of stocking density on the growth performance and digestive microbiota of broiler chickens","volume":"90","author":"Guardia","year":"2011","journal-title":"Poult Sci"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"e0275811","DOI":"10.1371\/journal.pone.0275811","article-title":"Stocking density-induced changes in growth performance, blood parameters, meat quality traits, and welfare of broiler chickens reared under semi-arid subtropical conditions","volume":"17","author":"Thema","year":"2022","journal-title":"PLoS One"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1007\/s00484-015-0964-3","article-title":"Environmental temperature and stocking density effects on acute phase proteins, heat shock protein 70, circulating corticosterone and performance in broiler chickens","volume":"59","author":"Najafi","year":"2015","journal-title":"Int J Biometeorol"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1080\/03079457.2014.1000820","article-title":"High stocking density as a predisposing factor for necrotic enteritis in broiler chicks","volume":"44","author":"Tsiouris","year":"2015","journal-title":"Avian Pathol"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1111\/jpn.13210","article-title":"Effect of dietary l-carnitine supplementation and energy level on oxidant\/antioxidant balance in laying hens subjected to high stocking density","volume":"104","author":"\u00c7etin","year":"2020","journal-title":"J Anim Physiol Anim Nutr (Berl)"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1080\/00071668.2017.1332405","article-title":"Influence of probiotic supplementation on immune response in broilers raised under hot climate","volume":"58","author":"Fathi","year":"2017","journal-title":"Br Poult Sci"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"101442","DOI":"10.1016\/j.psj.2021.101442","article-title":"Potential impact of stocking density on growth, carcass traits, indicators of biochemical and oxidative stress and meat quality of different broiler breeds","volume":"100","author":"Nasr","year":"2021","journal-title":"Poult Sci"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"871","DOI":"10.3390\/antiox11050871","article-title":"Effects of stocking density on growth performance, antioxidant status, and meat quality of finisher broiler chickens under high temperature","volume":"11","author":"Son","year":"2022","journal-title":"Antioxidants"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"341","DOI":"10.3390\/antiox13030341","article-title":"Effects of aspirin eugenol Ester on liver oxidative damage and energy metabolism in immune-stressed broilers","volume":"13","author":"Zhong","year":"2024","journal-title":"Antioxidants"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/j.aninu.2022.06.005","article-title":"Glycerol monolaurate attenuated immunological stress and intestinal mucosal injury by regulating the gut microbiota and activating AMPK\/Nrf2 signaling pathway in lipopolysaccharide-challenged broilers","volume":"10","author":"Kong","year":"2022","journal-title":"Anim Nutr"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"104003","DOI":"10.1016\/j.psj.2024.104003","article-title":"Oxidative stress in poultry production","volume":"103","author":"Oke","year":"2024","journal-title":"Poult Sci"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"498","DOI":"10.3390\/ani14030498","article-title":"Potential effects of garlic (Allium sativum L.) on the performance, immunity, gut health, anti-oxidant status, blood parameters, and intestinal microbiota of poultry: an updated comprehensive review","volume":"14","author":"Abd El-Ghany","year":"2024","journal-title":"Animals"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"103825","DOI":"10.1016\/j.psj.2024.103825","article-title":"Effect of dietary aspirin eugenol ester on the growth performance, antioxidant capacity, intestinal inflammation, and cecal microbiota of broilers under high stocking density","volume":"103","author":"Zhang","year":"2024","journal-title":"Poult Sci"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"e1303","DOI":"10.1002\/vms3.1303","article-title":"Effect of dietary lysophospholipid supplementation on growth performance, serum lipids, small intestine morphology and caeca microflora in broiler chickens","volume":"10","author":"Salari","year":"2023","journal-title":"Vet Med Sci"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"172","DOI":"10.3390\/vaccines10020172","article-title":"Role of physiology, immunity, microbiota, and infectious diseases in the gut health of poultry","volume":"10","author":"Wickramasuriya","year":"2022","journal-title":"Vaccine"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"4595","DOI":"10.3382\/ps\/pez152","article-title":"Effect of prebiotic and synbiotic supplementation in diet on growth performance, small intestinal morphology, stress, and bacterial population under high stocking density condition of broiler chickens","volume":"98","author":"Kridtayopas","year":"2019","journal-title":"Poult Sci"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"103970","DOI":"10.1016\/j.psj.2024.103970","article-title":"Dietary Kluyveromyces marxianus hydrolysate alters humoral immunity, jejunal morphology, cecal microbiota and metabolic pathways in broiler chickens raised under a high stocking density","volume":"103","author":"Rassmidatta","year":"2024","journal-title":"Poult Sci"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"103996","DOI":"10.1016\/j.psj.2024.103996","article-title":"Effects of oregano and\/or rosemary extracts on growth performance, digestive enzyme activities, cecal bacteria, tight junction proteins, and antioxidants-related genes in heat-stressed broiler chickens","volume":"103","author":"Madkour","year":"2024","journal-title":"Poult Sci"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"102623","DOI":"10.1016\/j.psj.2023.102623","article-title":"Effects of dietary chlorogenic acid on intestinal barrier function and the inflammatory response in broilers during lipopolysaccharide-induced immune stress","volume":"102","author":"Tan","year":"2023","journal-title":"Poult Sci"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"303","DOI":"10.3390\/ani13020303","article-title":"Dietary supplementation with Chlorogenic acid enhances antioxidant capacity, which promotes growth, jejunum barrier function, and cecum microbiota in broilers under high stocking density stress","volume":"13","author":"Liu","year":"2023","journal-title":"Animals-Basel"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1169375","DOI":"10.3389\/fphys.2023.1169375","article-title":"Effects of dietary chlorogenic acid on ileal intestinal morphology, barrier function, immune factors and gut microbiota of broilers under high stocking density stress","volume":"14","author":"Li","year":"2023","journal-title":"Front Physiol"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"101132","DOI":"10.1016\/j.psj.2021.101132","article-title":"Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density","volume":"100","author":"Wang","year":"2021","journal-title":"Poult Sci"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1111\/cea.12697","article-title":"Cyclooxygenase 2: its regulation, role and impact in airway inflammation","volume":"46","author":"Rumzhum","year":"2016","journal-title":"Clin Exp Allergy"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"2001","DOI":"10.3390\/microorganisms10102001","article-title":"Butyrate mitigates lipopolysaccharide-induced intestinal morphological changes in weanling piglets by regulating the microbiota and energy metabolism, and alleviating inflammation and apoptosis","volume":"10","author":"Han","year":"2022","journal-title":"Microorganisms"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.prostaglandins.2014.11.001","article-title":"Experimental ileitis alters prostaglandin biosynthesis in mesenteric lymphatic and blood vessels","author":"Rehal","year":"2015","journal-title":"Prostaglandins Other Lipid Mediat"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"7081","DOI":"10.1039\/c9fo00904c","article-title":"Effect of supplementation of nicotinamide and sodium butyrate on the growth performance, liver mitochondrial function and gut microbiota of broilers at high stocking density","volume":"10","author":"Wu","year":"2019","journal-title":"Food Funct"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1053\/j.gastro.2020.05.004","article-title":"Aspirin reduces colorectal tumor development in mice and gut microbes reduce its bioavailability and Chemopreventive effects","volume":"159","author":"Zhao","year":"2020","journal-title":"Gastroenterology"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1111\/bcpt.12006","article-title":"Evaluation of the in vivo anti-inflammatory and analgesic activity of a highly water-soluble aspirin conjugate","volume":"112","author":"Jacob","year":"2012","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/978-3-319-41342-6_3","article-title":"Eugenol and its role in chronic diseases","volume":"929","author":"Fujisawa","year":"2016","journal-title":"Adv Exp Med Biol"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1002\/bmc.3249","article-title":"In vivo and in vitro metabolism of aspirin eugenol ester in dog by liquid chromatography tandem mass spectrometry","volume":"29","author":"Shen","year":"2014","journal-title":"Biomed Chromatogr"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1186\/s12944-016-0369-2","article-title":"Lowering effects of aspirin eugenol ester on blood lipids in rats with high fat diet","volume":"15","author":"Karam","year":"2016","journal-title":"Lipids Health Dis"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1186\/s12917-018-1711-x","article-title":"Aspirin eugenol ester regulates cecal contents metabolomic profile and microbiota in an animal model of hyperlipidemia","volume":"14","author":"Ma","year":"2018","journal-title":"BMC Vet Res"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1007\/978-1-0716-1001-5_18","article-title":"Preservation and processing of intestinal tissue for the assessment of histopathology","volume":"2223","author":"Rieger","year":"2021","journal-title":"Methods Mol Biol"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.3390\/ani10081253","article-title":"Commercial poultry production stocking density influence on bird health and performance indicators","volume":"10","author":"Bergeron","year":"2020","journal-title":"Animals-Basel"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.3390\/foods10071505","article-title":"Effects of Scopoletin supplementation and stocking density on growth performance, antioxidant activity, and meat quality of Korean native broiler chickens","volume":"10","author":"Ha","year":"2021","journal-title":"Food Secur"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"101443","DOI":"10.1016\/j.psj.2021.101443","article-title":"Impacts of tea tree or lemongrass essential oils supplementation on growth, immunity, carcass traits, and blood biochemical parameters of broilers reared under different stocking densities","volume":"100","author":"Abo Ghanima","year":"2021","journal-title":"Poult Sci"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"18633","DOI":"10.1038\/s41598-024-68138-8","article-title":"Herbal extract dietary supplementation effect on growth performance and meat quality in broiler raised under two stocking densities","volume":"14","author":"Obeidat","year":"2024","journal-title":"Sci Rep"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1111\/jpn.13007","article-title":"Response of broiler chickens reared at high density to dietary supplementation with licorice extract and probiotic","volume":"103","author":"Rashidi","year":"2018","journal-title":"J Anim Physiol Anim Nutr"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"115606","DOI":"10.1016\/j.ecoenv.2023.115606","article-title":"Biomarkers of oxidative stress in broiler chickens attacked by lipopolysaccharide: a systematic review and meta-analysis","volume":"266","author":"Hu","year":"2023","journal-title":"Ecotoxicol Environ Saf"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1186\/s40104-021-00552-8","article-title":"Dietary seaweed-derived polysaccharides improve growth performance of weaned pigs through maintaining intestinal barrier function and modulating gut microbial populations","volume":"12","author":"Zou","year":"2021","journal-title":"J Anim Sci Biotechnol"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"253","DOI":"10.3390\/antiox12020253","article-title":"Encapsulated essential oils improve the growth performance of meat ducks by enhancing intestinal morphology, barrier function, antioxidant capacity and the Cecal microbiota","volume":"12","author":"Bao","year":"2023","journal-title":"Antioxidants"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"3024","DOI":"10.3390\/ani13193024","article-title":"Evaluation of the interaction between gum Arabic addition and stocking density on growth performance, carcass characteristics, and general health parameters of broiler chickens","volume":"13","author":"Al-Baadani","year":"2023","journal-title":"Animals-Basel"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"2910","DOI":"10.3390\/ani12212910","article-title":"Effect of selenium nanoparticles and mannan oligosaccharide supplementation on growth performance, stress indicators, and intestinal microarchitecture of broilers reared under high stocking density","volume":"12","author":"Rehman","year":"2022","journal-title":"Animals"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"2024001","DOI":"10.2141\/jpsa.2024001","article-title":"Trehalose supplementation effects on growth, intestinal morphology, gut bacteria, and footpad dermatitis of broiler chickens reared at high density","volume":"61","author":"Sooksridang","year":"2024","journal-title":"J Poult Sci"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1186\/s12917-022-03278-5","article-title":"Effects of Taraxacum mongolicum hand.-Mazz. (dandelion) on growth performance, expression of genes coding for tight junction protein and mucin, microbiota composition and short chain fatty acids in ileum of broiler chickens","volume":"18","author":"Mao","year":"2022","journal-title":"BMC Vet Res"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"830073","DOI":"10.3389\/fvets.2022.830073","article-title":"Assessment of antimicrobial effects on broiler gut barrier through histopathology and immunohistochemistry of tight-junction proteins","volume":"9","author":"Cuccato","year":"2022","journal-title":"Front Vet Sci"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"4203","DOI":"10.1016\/j.psj.2020.04.031","article-title":"Graded Eimeria challenge linearly regulated growth performance, dynamic change of gastrointestinal permeability, apparent ileal digestibility, intestinal morphology, and tight junctions of broiler chickens","volume":"99","author":"Teng","year":"2020","journal-title":"Poult Sci"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"102036","DOI":"10.1016\/j.psj.2022.102036","article-title":"Chlorogenic acid improves growth performance and intestinal health through autophagy-mediated nuclear factor erythroid 2-related factor 2 pathway in oxidatively stressed broilers induced by dexamethasone","volume":"101","author":"Liu","year":"2022","journal-title":"Poult Sci"},{"key":"ref59","doi-asserted-by":"publisher","first-page":"871713","DOI":"10.3389\/fimmu.2022.871713","article-title":"The role of intestinal mucosal barrier in autoimmune disease: a potential target","volume":"13","author":"An","year":"2022","journal-title":"Front Immunol"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"761981","DOI":"10.3389\/fimmu.2021.761981","article-title":"Interaction between commensal bacteria, immune response and the intestinal barrier in inflammatory bowel disease","volume":"12","author":"Chen","year":"2021","journal-title":"Front Immunol"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"107670","DOI":"10.1016\/j.intimp.2021.107670","article-title":"Food antigens exacerbate intestinal damage and inflammation following the disruption of the mucosal barrier","volume":"96","author":"Ma","year":"2021","journal-title":"Int Immunopharmacol"},{"key":"ref62","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1186\/s12951-023-01965-5","article-title":"Eucommia ulmoides polysaccharide modified nano-selenium effectively alleviated DSS-induced colitis through enhancing intestinal mucosal barrier function and antioxidant capacity","volume":"21","author":"Ye","year":"2023","journal-title":"J Nanobiotechnol"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"102528","DOI":"10.1016\/j.redox.2022.102528","article-title":"Impairments of intestinal arginine and NO metabolisms trigger aging-associated intestinal barrier dysfunction and \u2018inflammaging","volume":"58","author":"Brandt","year":"2022","journal-title":"Redox Biol"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"1277102","DOI":"10.3389\/fimmu.2023.1277102","article-title":"The role of intestinal microbes on intestinal barrier function and host immunity from a metabolite perspective","volume":"14","author":"Fu","year":"2023","journal-title":"Front Immunol"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.3390\/nu12041107","article-title":"The effect of probiotics on the production of short-chain fatty acids by human intestinal microbiome","volume":"12","author":"Markowiak-Kope\u0107","year":"2020","journal-title":"Nutrients"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"268","DOI":"10.4103\/ijmm.IJMM_19_373","article-title":"Gut microbiota and its mysteries","volume":"37","author":"Pushpanathan","year":"2019","journal-title":"Indian J Med Microbiol"},{"key":"ref67","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1136\/gutjnl-2015-309990","article-title":"The gut microbiota and host health: a new clinical frontier","volume":"65","author":"Marchesi","year":"2016","journal-title":"Gut"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"777456","DOI":"10.3389\/fmicb.2021.777456","article-title":"Effects of Clostridium butyricum on growth performance, gut microbiota and intestinal barrier function of broilers","volume":"12","author":"Li","year":"2021","journal-title":"Front Microbiol"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1186\/s40168-021-01040-x","article-title":"Joint contributions of the gut microbiota and host genetics to feed efficiency in chickens","volume":"9","author":"Wen","year":"2021","journal-title":"Microbiome"},{"key":"ref70","doi-asserted-by":"publisher","first-page":"102401","DOI":"10.1016\/j.psj.2022.102401","article-title":"Metagenomics analysis of the morphological aspects and bacterial composition of broiler feces","volume":"102","author":"Alvarenga","year":"2023","journal-title":"Poult Sci"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3389\/fmicb.2019.02126","article-title":"Efficacy of fecal sampling as a gut proxy in the study of chicken gut microbiota","volume":"10","author":"Yan","year":"2019","journal-title":"Front Microbiol"},{"key":"ref72","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1099\/ijsem.0.001793","article-title":"Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. Nov., sp. nov. and Dubosiella newyorkensis, gen. Nov., sp. nov., from the murine intestine, and emendation to the description of Faecalibacterium rodentium","volume":"67","author":"Cox","year":"2017","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref73","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1038\/s41522-023-00451-y","article-title":"Inulin-enriched Megamonas funiformis ameliorates metabolic dysfunction-associated fatty liver disease by producing propionic acid","volume":"9","author":"Yang","year":"2023","journal-title":"NPJ Biofilms Microbiomes"},{"key":"ref74","doi-asserted-by":"publisher","first-page":"2052","DOI":"10.3390\/nu16132052","article-title":"Monobutyrin can regulate the gut microbiota, which is beneficial for the development of intestinal barrier function and intestinal health in weaned mice","volume":"16","author":"Wang","year":"2024","journal-title":"Nutrients"},{"key":"ref75","doi-asserted-by":"publisher","first-page":"17434","DOI":"10.3390\/ijms242417434","article-title":"Protective effect and mechanism of aspirin eugenol Ester on lipopolysaccharide-induced intestinal barrier injury","volume":"24","author":"Tao","year":"2023","journal-title":"Int J Mol Sci"},{"key":"ref76","doi-asserted-by":"publisher","first-page":"2782","DOI":"10.1099\/ijsem.0.004107","article-title":"A taxonomic note on the genus Lactobacillus: description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae","volume":"70","author":"Zheng","year":"2020","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref77","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.3390\/ijms25021237","article-title":"Swine gastrointestinal microbiota and the effects of dietary amino acids on its composition and metabolism","volume":"25","author":"Liao","year":"2024","journal-title":"Int J Mol Sci"},{"key":"ref78","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1186\/s12866-023-03135-x","article-title":"Ligilactobacillus Salivarius improve body growth and anti-oxidation capacity of broiler chickens via regulation of the microbiota-gut-brain axis","volume":"23","author":"Yang","year":"2023","journal-title":"BMC Microbiol"}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2025.1638245\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T04:10:36Z","timestamp":1751861436000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2025.1638245\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,7]]},"references-count":78,"alternative-id":["10.3389\/fvets.2025.1638245"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2025.1638245","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,7]]},"article-number":"1638245"}}