{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:06:49Z","timestamp":1791493609112,"version":"4.3.4"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p><jats:italic>Dendrobium officinale<\/jats:italic> Kimura et Migo (<jats:italic>D. officinale<\/jats:italic>) is a valuable traditional Chinese herb, rich in bioactive compounds like polysaccharides and flavonoids. It is recognized for its immunomodulatory and antioxidant properties. However, its impact on canine metabolic health remains unexplored. This study investigated the effects of <jats:italic>Dendrobium officinale<\/jats:italic> supplementation on nutrient digestibility, blood biochemical parameters, antioxidant activity, and serum metabolomics in domestic poodles. Thirty-two ((5.20 \u00b1 0.26 kg)) healthy female poodles were randomly divided into four groups and fed diets containing 0% (control), 0.5%, 1%, or 2% <jats:italic>D. officinale<\/jats:italic> for 45 days. The results showed that <jats:italic>D. officinale<\/jats:italic> supplementation did not affect the apparent digestibility of dry matter (DM), crude protein (CP), crude fat (EE), crude ash (Ash), calcium (Ca) and phosphorus (P) in poodles (<jats:italic>p<\/jats:italic>\u202f&amp;gt;\u202f0.05). The peak IgM concentration (1.04 g\/L) was observed at a 1% supplementation level, while optimal TP and GLB levels (69.72 g\/L and 34.67 g\/L, respectively) were noted at 0.5% supplementation. The highest CAT activity (1.95 U\/mL) and T-AOC (7.12 U\/mL) were observed at a 1% level, while the highest GSH-Px activity (1630.56 U\/mL) was recorded at 2% supplementation level. Metabolomic analysis identified ten significantly altered serum metabolites involved in oxidative stress, inflammatory mediation, and energy metabolism. Key metabolic pathways influenced included arachidonic acid, caffeine, pyruvate, and amino acid metabolism.These findings suggest that <jats:italic>D. officinale<\/jats:italic> enhances immune and antioxidant function in poodles without affecting nutrient digestion, likely through modulation of critical metabolic pathways. A supplementation level of 0.5% to 1% is recommended to achieve these beneficial effects.<\/jats:p>","DOI":"10.3389\/fvets.2025.1642292","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T12:29:06Z","timestamp":1759148946000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Untargeted plasma metabolomics based on liquid chromatography-mass spectrometry for the analysis of Dendrobium officinale on canine immunity and antioxidant status"],"prefix":"10.3389","volume":"12","author":[{"given":"Jie","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoguo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baiguan","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihong","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongtao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoxian","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guosheng","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"6173","DOI":"10.1080\/21655979.2022.2043018","article-title":"Dietary supplementation of hemp oil in teddy dog: effect on apparent nutrient digestibility, blood biochemistry and metabolomics","volume":"13","author":"Xin","year":"2022","journal-title":"Bioengineered"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"611","DOI":"10.3969\/j.issn.1006-267x.2023.01.058","article-title":"Effects of dietary supplementing chitooligosaccharide on nutrient apparent digestibility, antioxidant capacity and intestinal microflora of dogs","volume":"35","author":"Yang","year":"2023","journal-title":"Chin J Anim Nutr"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.anifeedsci.2018.11.014","article-title":"Crude corn oil as dietary fat source for dogs","volume":"247","author":"Sabchuk","year":"2019","journal-title":"Anim Feed Sci Technol"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"7355","DOI":"10.3390\/molecules28217355","article-title":"The mechanisms of polysaccharides from tonic Chinese herbal medicine on the enhancement immune function: a review","volume":"28","author":"Xie","year":"2023","journal-title":"Molecules"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"100235","DOI":"10.1016\/j.fochx.2022.100235","article-title":"Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice","volume":"13","author":"Xie","year":"2022","journal-title":"Food Chem X"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1080\/13880209.2020.1787470","article-title":"Current advances of Dendrobium officinale polysaccharides in dermatology: a literature review","volume":"58","author":"Guo","year":"2020","journal-title":"Pharm Biol"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"e84522","DOI":"10.1590\/fst.84522","article-title":"Chemical constituents and nutritional health functions of Dendrobium nobile: a review","volume":"42","author":"Wang","year":"2022","journal-title":"Cienc Technol Aliment"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"920823","DOI":"10.3389\/fphar.2022.920823","article-title":"Dendrobium huoshanense cztang et sjcheng: a review of its traditional uses, phytochemistry, and pharmacology","volume":"13","author":"Gao","year":"2022","journal-title":"Front Pharmacol"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"726528","DOI":"10.3389\/fphar.2021.726528","article-title":"Traditional uses, phytochemistry, pharmacology, and quality control of Dendrobium officinale kimura et. 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