{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T16:10:18Z","timestamp":1763741418853,"version":"3.45.0"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Shigella<\/jats:italic>\n                      spp. are Gram-negative enteropathogens responsible for bacillary dysentery (shigellosis) in humans and animals. While the primary reservoirs of\n                      <jats:italic>Shigella<\/jats:italic>\n                      are historically recognized as humans and non-human primates, emerging evidence indicates an expanding host range encompassing diverse animal species. This study systematically characterizes a\n                      <jats:italic>Shigella<\/jats:italic>\n                      sp. strain isolated from diarrheic suckling mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Diarrheic suckling mice intestinal contents were collected and analyzed using 16S rRNA sequencing technology.\n                      <jats:italic>Shigella<\/jats:italic>\n                      sp. JZ001 strain was isolated, purified, and identified. Bacterial morphology was examined by transmission electron microscopy. Carbon source utilization profiles were determined using BIOLOG GENIII MicroPlates\u2122. Short-chain fatty acid (SCFA) composition was quantified by gas chromatography\u2013mass spectrometry (GC\u2013MS) following methyl-tert-butyl ether extraction. Polar lipid analysis was performed through two-dimensional thin-layer chromatography with detection using 10% ethanolic molybdatophosphoric acid. Whole genome sequencing was performed using a hybrid approach combining Illumina NovaSeq and Nanopore MinION platforms. Phylogenetic reconstruction was performed using maximum-likelihood algorithms implemented in MEGA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Rotavirus-SA11 infected suckling mice exhibited watery feces 4\u202fdays post-infection. Intestinal contents were analyzed using 16S rRNA sequencing technology, which revealed a significant increase in the genus\n                      <jats:italic>Shigella<\/jats:italic>\n                      in the RV-infected group. A\n                      <jats:italic>Shigella<\/jats:italic>\n                      strain was isolated and purified from the intestinal contents and designated as JZ001. Polyphasic characterization confirmed that strain JZ001 belongs to the Enterobacteriaceae family and is phylogenetically closest to\n                      <jats:italic>Shigella<\/jats:italic>\n                      spp. The genome consists of a single circular chromosome (5,329,126 bp; G\u202f+\u202fC content 50.65%) and one plasmid (1,09829 bp, G\u202f+\u202fC content 40.14%). Comparative genomic analysis identified 24 Salmonella pathogenicity island (SPI)-like regions. Substrate assimilation tests demonstrated metabolic versatility, including utilization of D-Maltose, D-Trehalose,\n                      <jats:italic>\u03b1<\/jats:italic>\n                      -D-Glucose, D-Mannose, D-Fructose, D-Galactose and Propionic Acid. Cellular fatty acid methyl ester (FAME) analysis identified predominant components as: Dodecanoic acid (12:0; 11.08%), Tetradecanoic acid (14:0; 12.00%), Hexadecanoic acid (16:0; 21.55%), cycloheptadecanoic acid (17:0 cyclo; 13.99%), Dodecanal (12:0 aldehyde; 22.32%). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, with notable absence of aminolipids and aminophospholipids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      First study of murine\n                      <jats:italic>Shigella<\/jats:italic>\n                      JZ001 offers insights into genomic plasticity and host adaptation mechanisms. The genomic dataset lays foundation for host-pathogen studies and facilitates developing murine models for therapy evaluation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2025.1686554","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T15:54:53Z","timestamp":1763740493000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole genome analysis of Shigella sp. 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