{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:28:00Z","timestamp":1778815680835,"version":"3.51.4"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Diagnosing feline nasal cavity diseases typically involves computed tomography, rhinoscopy, mycological examination, and histopathology. Culture-based bacteriological examination (cBE) is frequently performed, though its diagnostic and therapeutic relevance remains uncertain. Chronic rhinosinusitis (CRS), a diagnosis of exclusion, often responds poorly to standard antibiotics. This retrospective case series aimed to describe the correlation of cBE findings (1) across nasal diseases and (2) treatment responses in CRS cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Medical records of 25 cats with confirmed nasal disease using comprehensive diagnostics were reviewed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Included were 11 CRS cats, 7 with mycotic rhinitis, and 7 with nasal neoplasia. In 24\/25 cats, cBE was positive, with similar bacterial isolates across all groups. In CRS cats, treatment response did not consistently correlate with cBE results or antimicrobial susceptibility. 5\/11 CRS cats showed clinical improvement following a 21-day doxycycline course. The remaining 6\/11 CRS cats had not responded to previous targeted antibiotic therapy or empirical doxycycline for potential\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      species infection. 3\/6 cats responded only to immunosuppressive therapy notably cyclosporine in two CRS cats, representing the first report in feline medicine. Non-responders were 3\/6 CRS cats with marked turbinate destruction; 2\/3 tested positive for feline herpesvirus 1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      For diagnosing nasal diseases, cBE showed limited diagnostic relevance. In CRS, observations suggest that cBE may have limited diagnostic and therapeutic utility, leading to a clinical dilemma in interpretation. Empirical doxycycline treatment and immunosuppressive strategies, including cyclosporine, may be beneficial in selected CRS cases. Given the limitations of cBE, PCR testing for\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      species and viral pathogens may improve clinical decision-making in cats with CRS, both by helping to identify potential candidates for doxycycline treatment, and by assessing the risk of viral reactivation prior to initiating immunosuppressive therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2025.1687083","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T05:24:28Z","timestamp":1761888268000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Impact of culture-based bacteriological examination on diagnosis and treatment in cats with chronic nasal disease \u2014 Insights from a case series of 25 cats"],"prefix":"10.3389","volume":"12","author":[{"given":"Christin","family":"Emming","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jutta","family":"Verspohl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Beineke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"R\u00f6sch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e779","DOI":"10.1002\/vetr.779","article-title":"Antimicrobial resistance profiles of bacteria associated with lower respiratory tract infections in cats and dogs in England","volume":"190","author":"Mavrides","year":"2022","journal-title":"Vet Rec"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1481","DOI":"10.2460\/javma.23.04.0186","article-title":"Cats with idiopathic chronic rhinosinusitis that develop clinical signs before two years of age have more severe nasal Conchal lysis, sinus malformation, and more severe inflammation on histological examination","volume":"261","author":"Beauvois","year":"2023","journal-title":"J Am Vet Med Assoc"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1111\/jvim.14627","article-title":"Antimicrobial use guidelines for treatment of respiratory tract disease in dogs and cats: antimicrobial guidelines working Group of the International Society for companion animal infectious diseases","volume":"31","author":"Lappin","year":"2017","journal-title":"J Vet Intern Med"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1016\/j.jfms.2009.05.003","article-title":"Feline herpesvirus infection. ABCD guidelines on prevention and management","volume":"11","author":"Thiry","year":"2009","journal-title":"J Feline Med Surg"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"232","DOI":"10.3390\/vetsci11060232","article-title":"A scoping review of the evidence on prevalence of feline upper respiratory tract infections and associated risk factors","volume":"11","author":"Kennedy","year":"2024","journal-title":"Vet Sci"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"579","DOI":"10.2460\/javma.2005.227.579","article-title":"Assessment of infectious organisms associated with chronic rhinosinusitis in cats","volume":"227","author":"Johnson","year":"2005","journal-title":"J Am Vet Med Assoc"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1111\/jvim.16824","article-title":"The respiratory microbiota and its impact on health and disease in dogs and cats: a one health 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doxycycline","volume":"12","author":"Stavroulaki","year":"2022","journal-title":"Animals (Basel)"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.cvsm.2019.10.005","article-title":"Chronic Rhinitis in the Cat: An Update","volume":"50","author":"Reed","year":"2020","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.2460\/javma.230.7.1032","article-title":"Chronic nasal discharge in cats: 75 cases (1993-2004)","volume":"230","author":"Demko","year":"2007","journal-title":"J Am Vet Med Assoc"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jfms.2003.08.005","article-title":"Investigation of nasal disease in the cat--a retrospective study of 77 cases","volume":"6","author":"Henderson","year":"2004","journal-title":"J Feline Med 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Sousa","year":"2023","journal-title":"Vet Sci"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1111\/jvim.12562","article-title":"ACVIM consensus statement on therapeutic antimicrobial use in animals and antimicrobial resistance","volume":"29","author":"Weese","year":"2015","journal-title":"J Vet Intern Med"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1177\/0300985819891259","article-title":"Bacterial biogeography of the Colon in dogs with chronic inflammatory enteropathy","volume":"57","author":"Giaretta","year":"2020","journal-title":"Vet Pathol"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.cvsm.2010.12.006","article-title":"Intestinal microbiota of dogs and cats: a bigger world than we thought","volume":"41","author":"Suchodolski","year":"2011","journal-title":"Vet Clin North Am Small Anim 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Dis"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"149","DOI":"10.47665\/tb.38.2.050","article-title":"Response to doxycycline and oxytetracycline treatments in cats infected with Mycoplasma spp. and analysis of haemato-clinical findings and risk factors","volume":"38","author":"Cetin","year":"2021","journal-title":"Trop Biomed"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"68","DOI":"10.2174\/1874467210801010068","article-title":"Immunomodulatory properties of antibiotics","volume":"1","author":"Tauber","year":"2008","journal-title":"Curr Mol Pharmacol"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"109","DOI":"10.2174\/1874210601408010109","article-title":"Anti-inflammatory actions of adjunctive Tetracyclines and other agents in periodontitis and associated comorbidities","volume":"8","author":"Tilakaratne","year":"2014","journal-title":"Open Dent 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