{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T06:38:19Z","timestamp":1772779099938,"version":"3.50.1"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>Porcine Parvovirus (PPV) is a non-enveloped DNA virus that predominantly induces reproductive disorders in swine. The ongoing emergence of novel PPV variants and the frequent co-infections with other viruses have led to significant economic losses within the swine industry. This study, utilizing 31 previously reported complete PPV genome sequences, identified a conserved fragment of the PPV-NS1 gene through homology analysis. A TaqMan-based real-time quantitative PCR (TaqMan-qPCR) detection method was developed targeting this specific fragment. Sensitivity assessments determined a detection limit of 8.5\u202fcopies\/\u03bcL for standard plasmids. Specificity assessments showed no cross-reactivity with 10 other prevalent swine pathogens. The coefficients of variation for both intra-assay and inter-assay repeatability tests were both under 1%, demonstrating high reproducibility. Moreover, an analysis involving 32 clinical samples was conducted to compare the detection outcomes of the developed method with those obtained from a commercial kit. The findings demonstrated that the established method achieved a relative sensitivity of 100% and a relative compliance rate of 75%, suggesting its potential as an alternative to the commercial kit. In summary, the TaqMan-qPCR method developed in this study exhibits high sensitivity and specificity, making it ideal for detecting various clinical samples. It also provides a valuable tool for monitoring PPV and examining its epidemiological traits.<\/jats:p>","DOI":"10.3389\/fvets.2026.1736303","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T05:30:36Z","timestamp":1772775036000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of a TaqMan-based quantitative real-time PCR for the detection of porcine parvovirus"],"prefix":"10.3389","volume":"13","author":[{"given":"Zhiqiang","family":"Hu","sequence":"first","affiliation":[{"name":"College of Animal Science, Xichang University","place":["Xichang, China"]},{"name":"Key Laboratory of Animal Epidemic Disease Detection and Prevention in Panxi District, Xichang University","place":["Xichang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maosi","family":"Xu","sequence":"additional","affiliation":[{"name":"Shandong Zhongxin Food Group Co., 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