{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:05:27Z","timestamp":1789578327474,"version":"build-2803163510"},"reference-count":112,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"\n Introduction<\/jats:title>\n \n Achillea fragrantissima<\/jats:italic>\n (AFG) is known for its medicinal properties, while different Achillea species have shown both beneficial and detrimental impacts on male fertility. This investigation evaluated the effects of AFG oral administration on behavior, steroidogenesis, and immune-related gene expression in Sprague-Dawley male rats.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Forty-five mature rats were randomly grouped into: Control, Low-dose group (AFG-L: 500 mg\/kg extract), and High-dose group (AFG-H: 1000 mg\/kg extract). The AFG extract was orally administered for 30 days. Behavioral, hematological, reproductive, immune, and oxidative stress-related indices were evaluated.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The AFG administration at both doses significantly (\n p<\/jats:italic>\n &lt; 0.05) modulated the feeding and drinking, hole-board, swimming performance, tube dominance, and modified Y-maze tests outcomes. The AFG-L group displayed significant (\n p<\/jats:italic>\n &lt; 0.05) increases in final weight gain, sperm count, motility, and viable sperms %, with significant (\n p<\/jats:italic>\n &lt; 0.05) reductions in aberrant and dead sperms %. AFG significantly (\n p<\/jats:italic>\n &lt; 0.05) increased phagocytic index (PhI) and phagocytic %. However, erythrogram and leukocyte counts were not significantly altered. The AFG-L group displayed significant (\n p<\/jats:italic>\n &lt; 0.05) increases in serum testosterone (TEST), FSH, LH, and Estradiol (E2) hormones. Additionally, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-\u0251) concentrations were significantly decreased (\n p<\/jats:italic>\n &lt; 0.05). AFG extract administration significantly improved (\n p<\/jats:italic>\n &lt; 0.05) lipid profile, increased the testicular and splenic glutathione peroxidase (GPx), superoxide dismutase (SOD), total antioxidant capacity (TAC) levels, whereas the malondialdehyde (MDA), protein carbonyl (PCO), 8-hydroxy-2-deoxyguanosine (8OH2dG), and testicular lactate dehydrogenase (LDH) levels were significantly reduced (\n p<\/jats:italic>\n &lt; 0.05). AFG administration significantly (\n p<\/jats:italic>\n &lt; 0.05) upregulated mRNA expression of\n StAR, CYP11A1, CYP17A1<\/jats:italic>\n , and\n HSD17B3<\/jats:italic>\n in the testes, as well as\n CD3, CD4, CD20<\/jats:italic>\n , and\n IL-10<\/jats:italic>\n in the spleen, but expression levels of\n CYP19A1<\/jats:italic>\n and\n Caspase<\/jats:italic>\n -3 in testes, along with CD8 and Caspase-3 in spleen, were significantly (\n p<\/jats:italic>\n &lt; 0.05) downregulated. The AFG-L group maintained normal testicular architecture with preserved seminiferous tubules and only mild interstitial edema alongside mild splenic sinusoid dilatation with macrophage infiltration. In contrast, the AFG-H group exhibited significant histopathological alterations, including irregular seminiferous tubules, reduced mature sperm, and increased interstitial edema, while the spleen showed preserved lymphoid follicles with congested vasculature.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n The findings of the ongoing work demonstrate for the first time that\n A. fragrantissima<\/jats:italic>\n extract exerts reproductive and immune-enhancing outcomes in male rats, with the low dose exhibiting greater efficacy than the high dose, indicating a clear dose-dependent response.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fvets.2026.1746307","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T04:21:02Z","timestamp":1776313262000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Reproductive and immune-related consequences of Achillea fragrantissima (Forssk.) in male rats via modulation of steroidogenesis- and immune-related genes expression"],"prefix":"10.3389","volume":"13","author":[{"given":"Ehsan H.","family":"Abu-Zeid","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan M.","family":"Emam","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noura A.","family":"Abd-Allah","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"Sobh","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek","family":"Khamis","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham H.","family":"El-Attar","sequence":"additional","affiliation":[{"name":"General Administration for Medical Affairs, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shereen El.","family":"Abdel-Hamid","sequence":"additional","affiliation":[{"name":"Department of Behavior and Management of Animal, Poultry, and Aquatics, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim F.","family":"Rehan","sequence":"additional","affiliation":[{"name":"Department of Animal Behaviour and Husbandry, Faculty of Veterinary Medicine, Menoufia University","place":["Shebin Alkom, Menoufia, Egypt"]},{"name":"Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences","place":["Jastrz\u0119biec, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa","family":"Elnagar","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences","place":["Jastrz\u0119biec, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franti\u0161ek","family":"Zigo","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy","place":["Ko\u0161ice, Slovakia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Zigov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University","place":["Ko\u0161ice, Slovakia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esraa M.","family":"Fahmy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University","place":["Zagazig, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"313","DOI":"10.11648\/j.wjph.20240904.11","article-title":"Gender dynamics and the socioeconomic implications of infertility in Sierra Leone: a study on health, social stigmatization, and economic impact","volume":"9","author":"Sesay","year":"2024","journal-title":"World J Public 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