{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T15:05:36Z","timestamp":1780931136262,"version":"3.54.1"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    This study evaluated the effects of dietary \u03b2-mannanase supplementation on growth performance, intestinal development, nutrient digestibility, and cecal microbiota in growing broilers. A total of 160 chicks were assigned to one of two dietary treatments (8 replicates of 10 birds each) and were fed a basal corn\u2013soybean meal diet (Ctrl) or a basal diet supplemented with 150\u202fmg\/kg \u03b2-mannanase until day 21. With similar body weight, \u03b2-mannanase supplementation significantly reduced feed intake and improved the feed conversion ratio from day 1 to 21 (\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt;\u202f0.05). Compared with the Ctrl group, \u03b2-mannanase supplementation reduced chyme viscosity and upregulated jejunal mRNA expression of sodium-dependent glucose cotransporter 1 (\n                    <jats:italic>SGLT1<\/jats:italic>\n                    ) and fatty acid-binding protein 6 (\n                    <jats:italic>FABP6<\/jats:italic>\n                    ) (\n                    <jats:italic>p<\/jats:italic>\n                    &amp;lt;\u202f0.05). Although the addition of \u03b2-mannanase to the diet did not affect the length and weight of the duodenum and jejunum, it notably increased the villus height-to-crypt depth ratio in the jejunum (\n                    <jats:italic>p<\/jats:italic>\n                    \u202f&amp;lt;\u202f0.05), as well as the DNA content, a quantitative parameter of enterocytes, in both the duodenum and jejunum, contributing to a significant increase in the digestibility of dry matter and metabolizable energy. Regarding barrier integrity, \u03b2-mannanase supplementation decreased serum D-lactic acid and endotoxin levels and upregulated jejunal expression of zonula occludens-1 (\n                    <jats:italic>ZO-1<\/jats:italic>\n                    ),\n                    <jats:italic>occludin<\/jats:italic>\n                    , and\n                    <jats:italic>mucin-2<\/jats:italic>\n                    at day 21 (\n                    <jats:italic>p<\/jats:italic>\n                    \u202f&amp;lt;\u202f0.05). Furthermore, \u03b2-mannanase supplementation significantly increased the levels of secretory immunoglobulin A (sIgA) and reduced the expression of interleukin-1 beta (IL-1\u03b2) in the jejunum. Cecal microbiota analysis revealed that \u03b2-mannanase increased the relative abundance of\n                    <jats:italic>g_Alistipes_A_871400<\/jats:italic>\n                    ,\n                    <jats:italic>g_Lachnoclostridium_A_130679<\/jats:italic>\n                    ,\n                    <jats:italic>g_Enterocloster<\/jats:italic>\n                    , and\n                    <jats:italic>g_Comamonas_F_58925<\/jats:italic>\n                    . These findings indicate that dietary \u03b2-mannanase improves gut micro-ecology by reducing chyme viscosity, enhancing intestinal barrier function, and modulating microbiota composition, thereby promoting nutrient utilization and feed efficiency in growing broilers.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1751066","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:49:13Z","timestamp":1780930153000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Alterations in intestinal development and barrier function in response to dietary \u03b2-mannanase in growing broilers"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiaofei","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition, College of Animal Science and Technology, Ministry of Agriculture, Henan Agricultural University","place":["Zhengzhou, China"]},{"name":"Beijing Strowin Biotechnology Co., Ltd.","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition, College of Animal Science and Technology, Ministry of Agriculture, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingyu","family":"Xia","sequence":"additional","affiliation":[{"name":"Beijing Strowin Biotechnology Co., Ltd.","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition, College of Animal Science and Technology, Ministry of Agriculture, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Strowin Biotechnology Co., Ltd.","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Strowin Biotechnology Co., Ltd.","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Disease-Resistance Nutrition of China, Institute of Animal Nutrition, Ministry of Education, Sichuan Agricultural University","place":["Ya\u2019an, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejun","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Strowin Biotechnology Co., Ltd.","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition, College of Animal Science and Technology, Ministry of Agriculture, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaiyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biochemistry and Nutrition, College of Animal Science and Technology, Ministry of Agriculture, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.2527\/jas.2014-8147","article-title":"Carbohydrate composition and in vitro digestibility of dry matter and nonstarch polysaccharides in corn, sorghum, and wheat and coproducts from these grains","volume":"93","author":"Jaworski","year":"2015","journal-title":"J Anim Sci"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"104653","DOI":"10.1016\/j.psj.2024.104653","article-title":"Effects of replacing soybean meal with cottonseed meal on growth performance, carcass trait, intestinal development and intestinal microbiota of broiler chickens","volume":"104","author":"Yan","year":"2025","journal-title":"Poult Sci"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"txab160","DOI":"10.1093\/tas\/txab160","article-title":"Significance of single beta-mannanase supplementation on performance and energy utilization in broiler chickens, laying hens, turkeys, sows, and nursery-finish pigs: a meta-analysis and systematic review. 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