{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:19:57Z","timestamp":1770992397104,"version":"3.50.1"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Exposure to malathion has been linked to various toxicities that can affect nearly every organ in the human body. This study aimed to examine how malathion toxicity affects certain sero-biochemical and hematological parameters in chickens in order to determine the harmful effects on the blood, liver, and kidneys.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Two experiments were conducted as part of the study design. Experiments 1 and 2 were used to determine the LD50 value and the hazardous dose in chickens, respectively. In the first experiment, 10 groups of 30 birds each were used. Over a period of 4\u202fweeks, each group was divided into five subgroups (with six birds in each replicate), and one group served as a control. In the second experiment, six groups of birds and one control group of 10 chicks each were used (60\u202f+\u202f10 birds).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The LD\n                      <jats:sub>50<\/jats:sub>\n                      in this study was found to be 620\u202fmg\/kg of body weight. As the hazardous dose of malathion increased, the levels of hemoglobin (Hb) and packed cell volume (PCV) gradually decreased. Compared to the control group, all treatments had significantly higher red blood cells (RBC) and total leukocyte counts (TLC) (\n                      <jats:italic>p<\/jats:italic>\n                      &amp;lt;\u202f0.01). Albumin and glucose concentrations increased in tandem with the increases in malathion dosages. Significant increases were observed in aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl-transferase (GGT), and alanine aminotransferase (ALT) levels. Bilirubin, creatinine, and urea levels progressively increased when dosages were increased. Triglycerides (TG) and total cholesterol (TC) levels exhibited modest decreases as dosages increased. Toxic levels of malathion had a severe impact on magnesium and inorganic phosphate levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Hepatic, renal, and hematological functions are sensitive markers of malathion toxicity. The findings of the study provide a fundamental basis for understanding the acute toxicity of malathion in chickens. This study provides useful information for regulatory monitoring and risk assessment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1765548","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T13:42:02Z","timestamp":1770990122000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Malathion-induced hematotoxicity, hepatotoxicity, and nephrotoxicity in male chicks"],"prefix":"10.3389","volume":"13","author":[{"given":"Imad Mohamed Tahir","family":"Fadlalla","sequence":"first","affiliation":[{"name":"Department of Genetic Research, IRMC, Imam Abdulrahman Bin Faisal University","place":["Dammam, Saudi Arabia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid Musa","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, Sudan University of Science and Technology","place":["Khartoum, Sudan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Badr Hasab-Elrasoul","family":"Aljack","sequence":"additional","affiliation":[{"name":"Department of Poultry Sciences, College of Animal Production, Sudan University of Science and Technology","place":["Khartoum, Sudan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muzzamil","family":"Atta","sequence":"additional","affiliation":[{"name":"Department of Animal Resources, Ministry of Municipality and Environment","place":["Doha, Qatar"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1177\/09603271251389572","article-title":"Clinical utility of combined butyrylcholinesterase activity measurements in assessing acute malathion intoxication severity: a case 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Sudan","volume":"88","author":"ElGaddal","year":"1989","journal-title":"J Toxicol Med Hyg"},{"key":"ref13","volume-title":"Office of pesticide program","year":"1992"},{"key":"ref14","year":"2000"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"174","DOI":"10.3844\/ajptsp.2014.174-185","article-title":"Protective role of vitamin c and green tea extract on malathion-induced hepatotoxicity and nephrotoxicity in rats","volume":"9","author":"Elzoghby","year":"2014","journal-title":"Am J Pharmacol Toxicol"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/j.fct.2009.11.044","article-title":"Malathion-induced hepatotoxicity in rats: the effects of vitamins C and E","volume":"48","author":"Kalender","year":"2010","journal-title":"Food Chem Toxicol"},{"key":"ref17","volume-title":"Guidance document on acute Oral toxicity. 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(JDMIMSU)","author":"Jha","year":"2012"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"9417380","DOI":"10.1155\/2021\/9417380","article-title":"Malathion-induced Hematoxicity and its recovery pattern in Barbonymus gonionotus","volume":"2021","author":"Mrong","year":"2021","journal-title":"J Toxicol"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1038\/186172a0","article-title":"Influence of dietary intake of certain insecticides on the haemoglobin and erythrocyte content, of chick blood","volume":"186","author":"Srivastava","year":"1960","journal-title":"Nature"},{"key":"ref25","first-page":"103","article-title":"Acute toxicity bioassay of malathion (EC 50%) on the fish, Oreochromis mossambicus (tilapia) and associated histological alterations in gills","volume":"6","author":"Subburaj","year":"2018","journal-title":"J Entomol Zool Stud"},{"key":"ref26","first-page":"826","article-title":"OP toxic material causes polycythemia in 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animals","author":"Cornelius","year":"1986"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"17828","DOI":"10.1007\/s11356-015-5014-5","article-title":"Malathion-induced hepatotoxicity in male Wistar rats: biochemical and histopathological studies","volume":"22","author":"Flehi-Slim","year":"2015","journal-title":"Environ Sci Pollut Res"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1016\/j.jksus.2019.05.008","article-title":"Effects of black seed and thyme leaves dietary supplements against malathion insecticide -induced toxicity in experimental rat model","volume":"32","author":"Khalifa Fares","year":"2020","journal-title":"J King Saud Univ Sci"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1080\/15376520500191623","article-title":"Stimulatory effects of malathion on the key enzymes activities of insulin secretion in langerhans islets, glutamate dehydrogenase and 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