{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T15:09:51Z","timestamp":1783091391034,"version":"3.54.6"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Iron deficiency (ID) is known to increase cardiac workload and contribute to cardiac dysfunction in humans. However, its prevalence in canine heart disease remains poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective cohort study aimed to determine the prevalence of ID in dogs with myxomatous mitral valvular disease (MMVD) and evaluate its association with cardiac function. Dogs diagnosed with MMVD at the Veterinary Teaching Hospital of Nippon Veterinary and Life Science University between August 2020 and December 2025 were retrospectively evaluated. ID was assessed using transferrin saturation (TSAT) as a marker of iron metabolism. Dogs were stratified into three groups according to TSAT levels. Data from physical examinations, hematological and biochemical analyses, and echocardiographic assessments were analyzed. Statistical analyses were conducted to evaluate intergroup differences and correlations between TSAT levels and clinical variables.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ID was identified in 12% (9\/84) of the dogs. Dogs in the ID group demonstrated significantly higher stroke volume and cardiac output compared with those in the normal-TSAT group. In addition, TSAT was significantly and inversely correlated with cardiac output.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings indicate that ID is present in a subset of dogs with MMVD and is associated with increased cardiac output, likely reflecting reduced systemic vascular resistance. This hemodynamic alteration may represent a potential risk factor for the development of high-output heart failure in this population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1783638","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:12:24Z","timestamp":1776399144000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Prevalence of iron deficiency and its association with cardiac function in dogs with various stages of myxomatous mitral valvular disease"],"prefix":"10.3389","volume":"13","author":[{"given":"Arata","family":"Kitazawa","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryohei","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunosuke","family":"Yuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Satomi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimpei","family":"Kawai","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Teshima","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotaka","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine, School of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University","place":["Musashino-shi, Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"3273","DOI":"10.1038\/s41467-020-16923-0","article-title":"Iron imaging in myocardial infarction reperfusion injury","volume":"11","author":"Moon","year":"2020","journal-title":"Nat Commun"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"4584","DOI":"10.1002\/ehf2.13589","article-title":"Association of iron deficiency with incident cardiovascular diseases and mortality in the general population","volume":"8","author":"Schrage","year":"2021","journal-title":"ESC Heart Fail"},{"key":"ref3","first-page":"250","article-title":"Iron deficiency anemia","volume":"53","author":"Naigamwalla","year":"2012","journal-title":"Can Vet J"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1161\/CIRCHEARTFAILURE.111.960906","article-title":"Iron deficiency in 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