{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T13:46:25Z","timestamp":1782827185788,"version":"3.54.5"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Cannabidiol (CBD) is increasingly being used in veterinary medicine; however, its systemic molecular effects in dogs remain poorly characterized. This study employed label-free quantitative proteomics to compare the serum proteomic responses of healthy dogs (\n                    <jats:italic>n<\/jats:italic>\n                    \u202f=\u202f18) after 30\u202fdays of oral CBD delivery via three distinct matrices: hemp by-product feed pellets (F), CBD-infused oil (O), and semi-solid treat (SN). The verified chronic doses differed among the groups. Multivariate analysis revealed distinct formulation-specific proteomic signatures, with the F group clustered separately from the O and SN groups. Despite dose and matrix variations, all groups shared a core metabolic response characterized by downregulation of apolipoproteins (APOA4, APOC3, APOC1, and APOH) and upregulation of hemoglobin subunits (HBA and HBB), indicating CBD-mediated modulation of lipid metabolism and redox homeostasis. The high-exposure groups (O, SN) uniquely exhibited upregulation of proteins involved in vascular integrity and tissue scaffolding (e.g., TGFB1, PDGFRB, and VWF), while the SN group also showed induction of immunomodulatory and cytoprotective markers, such as clusterin (CLU). These findings demonstrate that the CBD delivery matrix critically influences systemic bioavailability and the scope of proteomic remodeling. Although all formulations engage core metabolic pathways, high-bioavailability formats induce additional signatures suggestive of vascular stabilization and stress resilience, providing a molecular rationale for optimizing CBD-based therapeutic formulations in canine medicine.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1803263","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T05:56:21Z","timestamp":1778651781000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Formulation-specific dose\u2013response in the serum proteome of healthy dogs following cannabidiol administration"],"prefix":"10.3389","volume":"13","author":[{"given":"Wutthiwong","family":"Theerapan","sequence":"first","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasithorn","family":"Limsuwan","sequence":"additional","affiliation":[{"name":"Institute of Food Research and Product Development, Kasetsart University","place":["Bangkok, Thailand"]},{"name":"Special Research Incubator Unit for Cannabis-Hemp and Phytochemicals in Veterinary Medicine, Faculty of Veterinary Medicine, Kasetsart University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jatuporn","family":"Rattanasrisomporn","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University","place":["Bangkok, Thailand"]},{"name":"Special Research Incubator Unit for Cannabis-Hemp and Phytochemicals in Veterinary Medicine, Faculty of Veterinary Medicine, Kasetsart University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sekkarin","family":"Ploypetch","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University","place":["Nakhon Pathom, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natthasit","family":"Tansakul","sequence":"additional","affiliation":[{"name":"Special Research Incubator Unit for Cannabis-Hemp and Phytochemicals in Veterinary Medicine, Faculty of Veterinary Medicine, Kasetsart University","place":["Bangkok, Thailand"]},{"name":"Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1146\/annurev-animal-081122-070236","article-title":"Scientific validation of cannabidiol for management of dog and cat diseases","volume":"11","author":"Corsato Alvarenga","year":"2023","journal-title":"Annu Rev Anim Biosci"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1539422","DOI":"10.3389\/fvets.2025.1539422","article-title":"Editorial: use of cannabis derivatives in veterinary medicine","volume":"12","author":"Temmerman","year":"2025","journal-title":"Front Vet Sci"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1666663","DOI":"10.3389\/fvets.2025.1666663","article-title":"Demographic features, health status, and behavioral changes associated with cannabidiol use in the dog aging 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