{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:05:44Z","timestamp":1793106344800,"version":"4.5.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Dry-preservation of gametes offers a promising alternative to cryobanking by potentially storing cells at ambient temperatures; however, successful preservation of whole oocytes remains limited by dehydration-induced cellular damages. This study evaluated whether intracellular delivery of trehalose via a membrane-permeable derivative, trehalose hexaacetate (6-O-Ac-Tre), could enhance dehydration tolerance of domestic cat cumulus\u2013oocyte complexes (COCs), a relevant model for mammalian fertility preservation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cat COCs were incubated with 0, 3, 10, or 30\u202fmM of 6-O-Ac-Tre to assess intracellular trehalose incorporation and cytotoxicity. Non-cytotoxic doses (3 and 10\u202fmM) were then tested for their ability to mitigate cellular damage following 10 or 15\u202fmin of microwave-assisted drying and rehydration, with outcomes including DNA integrity, mitochondrial membrane potential, cell membrane integrity, and meiotic maturation competence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overnight exposure to 6-O-Ac-Tre enabled dose-dependent intracellular accumulation of trehalose. However, cytotoxicity examination revealed that prolonged exposure to 30\u202fmM 6-O-Ac-Tre reduced survival and oocytes\u2019 meiotic and developmental competence (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f&amp;lt;\u202f0.05). Although DNA integrity was largely preserved after drying (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f&amp;gt;\u202f0.05), meiotic maturation of oocytes was severely compromised (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f&amp;lt;\u202f0.05). Pre-incubation with 3 or 10\u202fmM 6-O-Ac-Tre did not mitigate (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f&amp;gt;\u202f0.05) a reduction in mitochondrial membrane potential, cell membrane integrity, and meiotic competence.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings demonstrated that, while 6-O-Ac-Tre effectively delivered trehalose into COCs, this approach did not improve dehydration tolerance of whole oocytes under the conditions tested. Collective results reflected limitations of the 6-O-Ac-Tre delivery strategy owing to its potential cytotoxicity at higher concentrations. Alternative trehalose delivery conditions or approaches need to be explored to facilitate the development of effective dry-preservation strategies for oocytes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1805930","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T04:22:32Z","timestamp":1775535752000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Dehydration-based preservation of cat cumulus\u2013oocyte complexes is not improved by membrane-permeable trehalose"],"prefix":"10.3389","volume":"13","author":[{"given":"Pei-Chih","family":"Lee","sequence":"first","affiliation":[{"name":"Smithsonian\u2019s National Zoo and Conservation Biology Institute","place":["Washington, DC, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaywalee","family":"Chatdarong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Comizzoli","sequence":"additional","affiliation":[{"name":"Smithsonian\u2019s National Zoo and Conservation Biology Institute","place":["Washington, DC, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.fertnstert.2022.01.018","article-title":"The ART of cryopreservation and its changing 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