{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:20:37Z","timestamp":1791314437523,"version":"4.3.1"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"\n Wound infections caused by bacterial pathogens remain a major health concern, often delaying tissue repair and increasing morbidity. This study evaluated the phytochemical composition and antibacterial activity of methanolic leaf extracts from\n Aloe vera, Lawsonia inermis, Azadirachta indica, Curcuma longa<\/jats:italic>\n , and\n Achyranthes aspera<\/jats:italic>\n as potential natural alternatives for managing wound-associated infections. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, phenolics, saponins, and steroids in all extracts, with\n L. inermis<\/jats:italic>\n and\n C. longa<\/jats:italic>\n showing particularly high levels of phenolics, tannins, and flavonoids, while\n A. Indica<\/jats:italic>\n exhibited abundant alkaloids and saponins. From 123 wound samples, six bacterial species were isolated:\n Staphylococcus aureus<\/jats:italic>\n (38; 30.9%),\n Streptococcus agalactiae<\/jats:italic>\n (17; 13.8%),\n Proteus mirabilis<\/jats:italic>\n (22; 17.9%),\n Escherichia coli<\/jats:italic>\n (18; 14.6%),\n Pseudomonas aeruginosa<\/jats:italic>\n (15; 12.2%), and\n Klebsiella pneumoniae<\/jats:italic>\n (13; 10.6%). Antibacterial activity assessed using the agar well diffusion method showed that\n A. indica<\/jats:italic>\n produced the highest inhibition zones (12.5 \u00b1 0.5 mm to 20.4 \u00b1 0.9 mm), followed by\n C. longa<\/jats:italic>\n (11.6 \u00b1 0.5 mm to 19.2 \u00b1 0.7 mm). The positive control, ciprofloxacin (10 \u03bcg), exhibited inhibition zones ranging from 20.8 \u00b1 0.5 mm to 26.1 \u00b1 0.4 mm, whereas the negative control (10% DMSO) produced no inhibition. The observed antibacterial activity is likely due to the synergistic effects of bioactive phytochemicals, which disrupt bacterial cell membranes and inhibit bacterial growth. These findings indicate that\n A. indica<\/jats:italic>\n and\n C. longa<\/jats:italic>\n possess strong antibacterial potential and may serve as promising natural agents for managing wound infections caused by clinically relevant pathogens. Further\n in vivo<\/jats:italic>\n and toxicity studies are recommended to validate their therapeutic applicability.\n <\/jats:p>","DOI":"10.3389\/fvets.2026.1812558","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T04:45:53Z","timestamp":1776055553000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Methanolic plant extracts: emerging biotherapeutic alternatives for animal wound infection control"],"prefix":"10.3389","volume":"13","author":[{"given":"Safia","family":"Arbab","sequence":"first","affiliation":[{"name":"Medicine and Engineering Interdisciplinary Research Laboratory of Nursing & Materials, Nursing Key Laboratory of Sichuan Province, West China Hospital, West China School of Nursing, Sichuan University","place":["Chengdu, Sichuan, China"]},{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanif","family":"Ullah","sequence":"additional","affiliation":[{"name":"Medicine and Engineering Interdisciplinary Research Laboratory of Nursing & Materials, Nursing Key Laboratory of Sichuan Province, West China Hospital, West China School of Nursing, Sichuan University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid J.","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University","place":["Taif, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalaf F.","family":"Alsharif","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University","place":["Taif, Saudi 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