{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:00:05Z","timestamp":1781283605989,"version":"3.54.1"},"reference-count":96,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Hemolysis during storage compromises the quality of canine and feline packed red blood cell (pRBC) units by shortening unit shelf-life, reducing oxygen-carrying capacity, increasing the risk of non-immune-mediated hemolytic transfusion reactions, and necessitating the discard of units when stock is limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Donor- and unit-level determinants of percent hemolysis were investigated in a multicenter quality-control dataset comprising 5,583 canine and 3,176 feline pRBC units. Associations were assessed by univariable screening followed by multivariable statistical analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In both species, multivariable analysis indicated higher percent hemolysis with longer storage duration, older donor age, and lower unit packed cell volume (PCV). Beyond these common patterns, dogs exhibited higher percentage hemolysis in DEA 1 positive units and larger unit volume was associated with lower percentage hemolysis. Overall, 28.3% of canine units and 6.5% of feline units exceeded 0.8% hemolysis, whereas 16.5 and 4.0%, respectively, exceeded 1%. Threshold exceedance increased markedly from 22\u201328 days of storage onward.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Based on one of the largest multicenter veterinary datasets to date, these findings reaffirm that storage hemolysis increases with storage duration and is further shaped by donor- and unit-level characteristics. These results may support more precise donor selection and unit release policies and reinforce hemolysis testing of units stored for \u226528 days.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1816048","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T16:25:55Z","timestamp":1781281555000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Donor- and unit-specific factors influencing hemolysis in stored canine and feline packed red blood cells"],"prefix":"10.3389","volume":"13","author":[{"given":"Pablo","family":"Reguera","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, A\u00fana Especialidades Veterinarias IVC Evidensia","place":["Valencia, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, A\u00fana Especialidades Veterinarias IVC Evidensia","place":["Valencia, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreia","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Banco de Sangue Animal","place":["Porto, Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Queiros","sequence":"additional","affiliation":[{"name":"Banco de Sangue Animal","place":["Porto, Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Banco de Sangue Animal","place":["Porto, Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Mesa","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, A\u00fana Especialidades Veterinarias IVC Evidensia","place":["Valencia, Spain"]},{"name":"Animal Blood Bank","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1016\/j.cvsm.2020.07.001","article-title":"Small Animal Transfusion Medicine","volume":"50","author":"Kuo","year":"2020","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"793","DOI":"10.3390\/metabo13070793","article-title":"Red blood cell metabolism in vivo and in vitro","volume":"13","author":"D\u2019Alessandro","year":"2023","journal-title":"Meta"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1111\/vec.12252","article-title":"Red blood cell storage lesion","volume":"25","author":"Obrador","year":"2015","journal-title":"J Vet Emerg Crit Care"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1111\/vec.13044","article-title":"Association of Veterinary Hematology and Transfusion Medicine (AVHTM) transfusion reaction small animal consensus statement (TRACS) part 2: prevention and monitoring","volume":"31","author":"Davidow","year":"2021","journal-title":"J Vet Emerg Crit Care"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1016\/j.athoracsur.2018.08.073","article-title":"Real age: red blood cell aging during storage","volume":"107","author":"Koch","year":"2019","journal-title":"Ann Thorac Surg"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1111\/j.1939-1676.1997.tb00065.x","article-title":"Evaluation of canine red blood cells stored in a saline, adenine, and glucose solution for 35 days","volume":"11","author":"Wardrop","year":"1997","journal-title":"J Vet Intern Med"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1111\/vcp.12163","article-title":"Effects of four additive solutions on canine Leukoreduced red cell concentrate quality during storage","volume":"43","author":"Lacerda","year":"2014","journal-title":"Vet Clin Pathol"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1111\/vec.12770","article-title":"In vitro hemolysis of stored units of canine packed red blood cells","volume":"28","author":"Ferreira","year":"2018","journal-title":"J Vet Emerg Crit Care"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.1111\/jvim.15056","article-title":"In vitro assessment of quality of citrate-phosphate-dextrose-Adenine-1 preserved feline blood collected by a commercial closed system","volume":"32","author":"Snead","year":"2018","journal-title":"J Vet Intern Med"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1186\/s12917-018-1458-4","article-title":"In vitro quality control analysis after processing and during storage of feline packed red blood cell units","volume":"14","author":"Giorgi","year":"2018","journal-title":"BMC Vet Res"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"105396","DOI":"10.1016\/j.tvjl.2019.105396","article-title":"Hematological, biochemical and microbiological evaluation of feline whole blood units collected using an open system and stored for 35 days","volume":"254","author":"Giorgi","year":"2019","journal-title":"Vet J"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1111\/vcp.12865","article-title":"Evaluation of hematologic, biochemical, and blood gas variables in stored canine packed red blood cells, and the impact of storage time on blood recipients","volume":"49","author":"de Almeida","year":"2020","journal-title":"Vet Clin Pathol"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"27","DOI":"10.2450\/2019.0217-18","article-title":"Red blood cell storage lesion: causes and potential clinical consequences","volume":"17","author":"Yoshida","year":"2019","journal-title":"Blood Transfus"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1089\/ars.2009.2799","article-title":"Toxicological consequences of extracellular Hemoglobin: biochemical and physiological perspectives","volume":"12","author":"Buehler","year":"2010","journal-title":"Antioxid Redox Signal"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1653","DOI":"10.1001\/jama.293.13.1653","article-title":"The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin: a novel mechanism of human disease","volume":"293","author":"Rother","year":"2005","journal-title":"JAMA"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1152\/jappl.1949.1.7.469","article-title":"Clinical experience with Hemoglobin-saline solutions","volume":"1","author":"Amberson","year":"1949","journal-title":"J Appl Physiol"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1002\/cpt197823173","article-title":"A clinical safety trial of stroma-free hemoglobin","volume":"23","author":"Savitsky","year":"1978","journal-title":"Clin Pharmacol Ther"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"e2064","DOI":"10.1038\/cddis.2015.392","article-title":"Hemoglobinuria-related acute kidney injury is driven by intrarenal oxidative reactions triggering a heme toxicity response","volume":"7","author":"Deuel","year":"2016","journal-title":"Cell Death Dis"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1084\/jem.86.4.339","article-title":"The pathogenesis of the renal injury produced in the dog by Hemoglobin or Methemoglobin","volume":"86","author":"Harrison","year":"1947","journal-title":"J Exp Med"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"1483","DOI":"10.1056\/NEJMcibr023045","article-title":"Hemoglobin and the paracrine and endocrine functions of nitric oxide","volume":"348","author":"Schechter","year":"2003","journal-title":"N Engl J Med"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"2619","DOI":"10.1182\/asheducation-2011.1.475","article-title":"Vascular effects of the red cell storage lesion","volume":"53","author":"Alexander","year":"2013","journal-title":"Transfusion"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1089\/152308601300185250","article-title":"Redox reactions of Hemoglobin and myoglobin: biological and toxicological implications","volume":"3","author":"Alayash","year":"2001","journal-title":"Antioxid Redox Signal"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1229","DOI":"10.1056\/NEJMoa070403","article-title":"Duration of red-cell storage and complications after cardiac surgery","volume":"358","author":"Koch","year":"2008","journal-title":"N Engl J Med"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"2495","DOI":"10.1111\/jvim.17175","article-title":"A prospective multicenter observational study assessing incidence and risk factors for acute blood transfusion reactions in dogs","volume":"38","author":"Hall","year":"2024","journal-title":"J Vet Intern Med"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1111\/trf.12607","article-title":"Transfusion of older stored blood worsens outcomes in canines depending on the presence and severity of pneumonia","volume":"54","author":"Wang","year":"2014","journal-title":"Transfusion"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"1663","DOI":"10.1182\/blood-2012-10-462945","article-title":"Mortality increases after massive exchange transfusion with older stored blood in canines with experimental pneumonia","volume":"121","author":"Solomon","year":"2013","journal-title":"Blood"},{"key":"ref27","first-page":"187","volume-title":"American Association of Blood Banks Technical Manual","author":"Kakaiya","year":"2012"},{"key":"ref28","first-page":"187","volume-title":"Guide to the Preparation, Use and Quality Assurance of Blood Components","year":"2011"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.freeradbiomed.2014.07.040","article-title":"Heritability of glutathione and related metabolites in stored red blood cells","volume":"76","author":"van","year":"2014","journal-title":"Free Radic Biol Med"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"2055","DOI":"10.1111\/trf.12605","article-title":"The heritability of metabolite concentrations in stored human red blood cells","volume":"54","author":"van","year":"2014","journal-title":"Transfusion"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1178","DOI":"10.1111\/trf.12992","article-title":"The heritability of Hemolysis in stored human red blood cells","volume":"55","author":"van","year":"2015","journal-title":"Transfusion"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"116","DOI":"10.2450\/2017.0326-16","article-title":"Red blood cell components: time to revisit the sources of variability","volume":"15","author":"Sparrow","year":"2017","journal-title":"Blood Transfus"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1002\/prca.201500128","article-title":"Donor-variation effect on red blood cell storage lesion: a close relationship emerges","volume":"10","author":"Tzounakas","year":"2016","journal-title":"Proteomics Clin Appl"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"258","DOI":"10.2450\/2019.0056-19","article-title":"Diversity in a blood bag: application of omics technologies to inform precision transfusion medicine","volume":"17","author":"Kanias","year":"2019","journal-title":"Blood Transfus"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"12281","DOI":"10.3390\/ijms222212281","article-title":"The post-storage performance of RBCs from Beta-thalassemia trait donors is related to their storability profile","volume":"22","author":"Anastasiadi","year":"2021","journal-title":"Int J Mol Sci"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"3943","DOI":"10.3390\/ijms22083943","article-title":"Current understanding of the relationship between blood donor variability and blood component quality","volume":"22","author":"Hadjesfandiari","year":"2021","journal-title":"Int J Mol Sci"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"e146077","DOI":"10.1172\/JCI146077","article-title":"Multiple-ancestry genome-wide association study identifies 27 loci associated with measures of Hemolysis following blood storage","volume":"131","author":"Grier","year":"2021","journal-title":"J Clin Invest"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"112","DOI":"10.3324\/haematol.2020.273946","article-title":"Beta thalassemia minor is a beneficial determinant of red blood cell storage lesion","volume":"107","author":"Tzounakas","year":"2022","journal-title":"Haematologica"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"e152598","DOI":"10.1172\/jci.insight.152598","article-title":"Donor genetic and nongenetic factors affecting red blood cell transfusion effectiveness","volume":"7","author":"Roubinian","year":"2022","journal-title":"JCI Insight"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"907444","DOI":"10.3389\/fphys.2022.907444","article-title":"Innate variability in physiological and omics aspects of the Beta thalassemia trait-specific donor variation effects","volume":"13","author":"Anastasiadi","year":"2022","journal-title":"Front Physiol"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1001\/jamainternmed.2016.3324","article-title":"Association of Blood Donor age and sex with recipient survival after red blood cell transfusion","volume":"176","author":"Chass\u00e9","year":"2016","journal-title":"JAMA Intern Med"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1111\/trf.13537","article-title":"Effect of storage time and donor sex of transfused red blood cells on 1-year survival in patients undergoing cardiac surgery: an observational study","volume":"56","author":"Cholette","year":"2016","journal-title":"Transfusion"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"2571","DOI":"10.1111\/trf.13745","article-title":"Testosterone-dependent sex differences in red blood cell hemolysis in storage, stress, and disease","volume":"56","author":"Kanias","year":"2016","journal-title":"Transfusion"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.1182\/bloodadvances.2017004820","article-title":"Ethnicity, sex, and age are determinants of red blood cell storage and stress Hemolysis: results of the REDS-III RBC-omics study","volume":"1","author":"Kanias","year":"2017","journal-title":"Blood Adv"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1111\/trf.14987","article-title":"Intradonor reproducibility and changes in Hemolytic variables during red blood cell storage: results of recall phase of the REDS-III RBC-omics study","volume":"59","author":"Lanteri","year":"2019","journal-title":"Transfusion"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.1001\/jama.2019.7084","article-title":"Association of blood donor sex and prior pregnancy with mortality among red blood cell transfusion recipients","volume":"321","author":"Edgren","year":"2019","journal-title":"JAMA"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"2633","DOI":"10.1111\/trf.16017","article-title":"Donor-dependent aging of young and old red blood cell subpopulations: metabolic and functional heterogeneity","volume":"60","author":"Mykhailova","year":"2020","journal-title":"Transfusion"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.3324\/haematol.2020.246603","article-title":"Donor sex, age and ethnicity impact stored red blood cell antioxidant metabolism through mechanisms in part explained by glucose 6-phosphate dehydrogenase levels and activity","volume":"106","author":"D\u2019Alessandro","year":"2021","journal-title":"Haematologica"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1186\/s12864-022-08461-4","article-title":"Sex-specific genetic modifiers identified susceptibility of cold stored red blood cells to osmotic Hemolysis","volume":"23","author":"Fang","year":"2022","journal-title":"BMC Genomics"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"150750","DOI":"10.1016\/j.tmrv.2023.150750","article-title":"Red blood cell storage: from genome to exposome \u2014 towards personalized transfusion medicine","volume":"37","author":"D\u2019Alessandro","year":"2023","journal-title":"Transfus Med Rev"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1111\/trf.17749","article-title":"Estimated median density identifies donor age and sex differences in red blood cell biological age","volume":"64","author":"Mykhailova","year":"2024","journal-title":"Transfusion"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1111\/vox.13602","article-title":"Red cell concentrates from teen male donors contain poor-quality biologically older cells","volume":"119","author":"Mykhailova","year":"2024","journal-title":"Vox Sang"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1111\/trf.14998","article-title":"Frequent blood donations alter susceptibility of red blood cells to storage- and stress-induced hemolysis","volume":"59","author":"Kanias","year":"2019","journal-title":"Transfusion"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1111\/trf.15743","article-title":"Genetic and behavioral modification of hemoglobin and iron status among first-time and high-intensity blood donors","volume":"60","author":"Mast","year":"2020","journal-title":"Transfusion"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1111\/trf.16168","article-title":"Blood donor obesity is associated with changes in red blood cell metabolism and susceptibility to Hemolysis in cold storage and in response to osmotic and oxidative stress","volume":"61","author":"Hazegh","year":"2021","journal-title":"Transfusion"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.tmrv.2003.10.003","article-title":"L-carnitine and its possible role in red cell and platelet storage","volume":"18","author":"Sweeney","year":"2004","journal-title":"Transfus Med Rev"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"2248","DOI":"10.1111\/trf.12106","article-title":"Addition of ascorbic acid solution to stored murine red blood cells increases posttransfusion recovery and decreases microparticles and alloimmunization","volume":"53","author":"Stowell","year":"2013","journal-title":"Transfusion"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"376","DOI":"10.2450\/2014.0266-13","article-title":"Storing red blood cells with vitamin C and N-acetylcysteine prevents oxidative stress-related lesions: a metabolomics overview","volume":"12","author":"Pallotta","year":"2014","journal-title":"Blood Transfus"},{"key":"ref59","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1159\/000486605","article-title":"Vitamin E analogue protects red blood cells against storage-induced oxidative damage","volume":"45","author":"Antosik","year":"2018","journal-title":"Transfus Med Hemother"},{"key":"ref60","first-page":"183","article-title":"Characteristics of resveratrol and serotonin on antioxidant protection of human erythrocytes","volume":"15","author":"Huyut","year":"2018","journal-title":"Int J Med Sci"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.1111\/trf.15810","article-title":"Impact of taurine on red blood cell metabolism and implications for blood storage","volume":"60","author":"Bertolone","year":"2020","journal-title":"Transfusion"},{"key":"ref62","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1111\/trf.15811","article-title":"Ethyl glucuronide, a marker of alcohol consumption, correlates with metabolic markers of oxidant stress but not with hemolysis in stored red blood cells from healthy blood donors","volume":"60","author":"D\u2019Alessandro","year":"2020","journal-title":"Transfusion"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1111\/trf.15833","article-title":"Additive effects of blood donor smoking and gamma irradiation on outcome measures of red blood cell transfusion","volume":"60","author":"DeSimone","year":"2020","journal-title":"Transfusion"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.1111\/trf.15812","article-title":"Nicotine exposure increases markers of oxidant stress in stored red blood cells from healthy donor volunteers","volume":"60","author":"Stefanoni","year":"2020","journal-title":"Transfusion"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"e146778","DOI":"10.1172\/jci.insight.146175","article-title":"Blood donor exposome and impact of common drugs on red blood cell metabolism","volume":"6","author":"Nemkov","year":"2021","journal-title":"JCI Insight"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"2391","DOI":"10.3390\/cells11152391","article-title":"A\u00e7a\u00ec (Euterpe oleracea) extract protects human erythrocytes","volume":"11","author":"Remigante","year":"2022","journal-title":"Cells"},{"key":"ref67","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.tracli.2017.08.001","article-title":"Oxidative stress and antioxidant Defenses during blood processing and storage of erythrocyte concentrates","volume":"25","author":"Bardyn","year":"2018","journal-title":"Transfus Clin Biol"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"722896","DOI":"10.3389\/fphys.2021.722896","article-title":"Deformability of stored red blood cells","volume":"12","author":"Barshtein","year":"2021","journal-title":"Front Physiol"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1111\/vec.70002","article-title":"Effect of N&lt;b&gt;-&lt;\/b&gt;acetylcysteine, ascorbic acid, and a vitamin E analog on oxidative and storage lesions in canine packed red blood cells","volume":"35","author":"Roque-Torres","year":"2025","journal-title":"J Vet Emerg Crit Care"},{"key":"ref70","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1053\/tmrv.2002.29404","article-title":"Red blood cell Hemolysis during processing","volume":"16","author":"Sowemimo-Coker","year":"2002","journal-title":"Transfus Med Rev"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1111\/j.1537-2995.2004.03315.x","article-title":"Visual assessment of Hemolysis in red blood cell units and segments can be deceptive","volume":"44","author":"Janatpour","year":"2004","journal-title":"Transfusion"},{"key":"ref72","doi-asserted-by":"publisher","first-page":"47","DOI":"10.4103\/0973-6247.33446","article-title":"Red cell hemolysis during processing and storage","volume":"1","author":"Sawant","year":"2007","journal-title":"Asian J Transfus Sci"},{"key":"ref73","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1111\/j.1423-0410.2008.01097.x","article-title":"Erythrocyte storage in hypertonic (SAGM) or isotonic (PAGGSM) conservation medium: influence on cell properties","volume":"95","author":"Zehnder","year":"2008","journal-title":"Vox Sang"},{"key":"ref74","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/j.cbpc.2007.04.004","article-title":"The effects of anticoagulants on hematological indices and blood cell morphology of common carp (Cyprinus carpio L.)","volume":"146","author":"Walencik","year":"2007","journal-title":"Comp Biochem Physiol C Toxicol Pharmacol"},{"key":"ref75","doi-asserted-by":"publisher","first-page":"20","DOI":"10.11648\/j.ijbse.20130102.11","article-title":"Prolong storage of blood in EDTA has an effect on the morphology and osmotic fragility of erythrocytes","volume":"1","author":"Antwi-Baffour","year":"2014","journal-title":"Int J Biomed Sci Eng"},{"key":"ref76","first-page":"411","article-title":"Osmotic fragility of erythrocytes of dogs and cats in health and in certain hematologic disorders","volume":"63","author":"Jain","year":"1973","journal-title":"Cornell Vet"},{"key":"ref77","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1111\/vox.12783","article-title":"Sex-mismatched red blood cell transfusions and mortality: a systematic review and meta-analysis","volume":"114","author":"Zeller","year":"2019","journal-title":"Vox Sang"},{"key":"ref78","doi-asserted-by":"publisher","first-page":"e2123942","DOI":"10.1001\/jamanetworkopen.2021.23942","article-title":"Association of Blood Donor sex and age with Outcomes in very low-birth-weight infants receiving blood transfusion","volume":"4","author":"Patel","year":"2021","journal-title":"JAMA Netw Open"},{"key":"ref79","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1111\/j.1537-2995.2007.01159.x","article-title":"Blood use by inpatient elderly population in the United States","volume":"47","author":"Anderson","year":"2007","journal-title":"Transfusion"},{"key":"ref80","doi-asserted-by":"publisher","first-page":"6675","DOI":"10.1182\/blood-2011-08-371849","article-title":"Transfusion of human volunteers with older, stored red blood cells produces extravascular hemolysis and circulating non-transferrin-bound iron","volume":"118","author":"Hod","year":"2011","journal-title":"Blood"},{"key":"ref81","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1111\/vox.12378","article-title":"Assessing the influence of component processing and donor characteristics on quality of red cell concentrates using quality control data","volume":"111","author":"Jordan","year":"2016","journal-title":"Vox Sang"},{"key":"ref82","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.transci.2019.04.023","article-title":"Biological mechanisms implicated in adverse outcomes of sex mismatched transfusions","volume":"58","author":"Alshalani","year":"2019","journal-title":"Transfus Apher Sci"},{"key":"ref83","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1111\/j.1742-4658.2009.07472.x","article-title":"Biopreservation of red blood cells \u2014 the struggle with Hemoglobin oxidation","volume":"277","author":"Kanias","year":"2010","journal-title":"FEBS J"},{"key":"ref84","doi-asserted-by":"publisher","first-page":"238","DOI":"10.2450\/2015.0055-15","article-title":"Blood banking-induced alteration of red blood cell oxygen release ability","volume":"14","author":"Li","year":"2016","journal-title":"Blood Transfus"},{"key":"ref85","doi-asserted-by":"publisher","first-page":"224","DOI":"10.2450\/2020.0141-20","article-title":"Sex-related aspects of the red blood cell storage lesion","volume":"19","author":"Tzounakas","year":"2021","journal-title":"Blood Transfus"},{"key":"ref86","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1111\/j.1423-0410.2010.01439.x","article-title":"Menopausal status affects the susceptibility of stored RBCs to mechanical stress","volume":"100","author":"Raval","year":"2011","journal-title":"Vox Sang"},{"key":"ref87","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1046\/j.1537-2995.1976.16276155103.x","article-title":"Distribution of progesterone and its effect on human blood during storage","volume":"16","author":"DeVenuto","year":"1976","journal-title":"Transfusion"},{"key":"ref88","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.1056\/NEJMoa2211523","article-title":"Effect of donor sex on recipient mortality in transfusion","volume":"388","author":"Chass\u00e9","year":"2023","journal-title":"N Engl J Med"},{"key":"ref89","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1053\/tmrv.2002.35212","article-title":"Storage of red blood cells: new approaches","volume":"16","author":"Hess","year":"2002","journal-title":"Transfus Med Rev"},{"key":"ref90","doi-asserted-by":"publisher","first-page":"10991","DOI":"10.3390\/ijms231910991","article-title":"Antioxidant activity of quercetin in a H2O2-induced oxidative stress model in red blood cells: functional role of band 3 protein","volume":"23","author":"Remigante","year":"2022","journal-title":"Int J Mol Sci"},{"key":"ref91","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1111\/jvim.16039","article-title":"Effects of leukoreduction on storage lesions in whole blood and blood components of dogs","volume":"35","author":"Stefani","year":"2021","journal-title":"J Vet Intern Med"},{"key":"ref92","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.1111\/jvim.17031","article-title":"Effect of leukoreduction on the omics phenotypes of canine packed RBC units","volume":"38","author":"Miglio","year":"2024","journal-title":"J Vet Intern Med"},{"key":"ref93","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1111\/j.1939-1676.2010.0561.x","article-title":"Effect of leukoreduction on transfusion-induced inflammation in dogs","volume":"24","author":"McMichael","year":"2010","journal-title":"J Vet Intern Med"},{"key":"ref94","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1182\/blood-2013-11-539353","article-title":"Washing older blood units before transfusion reduces plasma iron and improves outcomes in experimental canine pneumonia","volume":"123","author":"Cort\u00e9s-Puch","year":"2014","journal-title":"Blood"},{"key":"ref95","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1111\/jvim.16340","article-title":"Effect of washing units of canine red blood cells on storage lesions","volume":"36","author":"Coll","year":"2022","journal-title":"J Vet Intern Med"},{"key":"ref96","doi-asserted-by":"publisher","first-page":"3537","DOI":"10.1111\/jvim.17231","article-title":"Donor and unit-specific factors influencing Hemolysis in stored canine and feline packed red blood cells. In research communications of the 34th ECVIM-CA congress; proceedings of the 34th European College of Veterinary Internal Medicine\u2014Companion Animals (ECVIM-CA) annual congress, Lyon, France, 5\u20137 September 2024","volume":"38","author":"Reguera Burgos","year":"2024","journal-title":"J Vet Intern Med"}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1816048\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T16:25:58Z","timestamp":1781281558000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1816048\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,12]]},"references-count":96,"alternative-id":["10.3389\/fvets.2026.1816048"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2026.1816048","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,12]]},"article-number":"1816048"}}