{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:57:57Z","timestamp":1777636677401,"version":"3.51.4"},"reference-count":19,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Neonatal calf diarrhea caused by bovine rotavirus (BRV) remains a major health and economic burden in cattle production, and current oral antibody approaches require frequent dosing because of poor systemic persistence. This study investigated whether a single oral dose of a BRV-specific antibody engineered with an immunoglobulin Fc region\u2014designed to extend circulatory half-life\u2014could be detected in the blood of newborn calves for up to 28\u202fdays. The antibody construct showed high-affinity binding to BRV antigen and effectively neutralized viral infection in cell culture. In a controlled calf trial, neonatal Holstein calves under 24\u202fh of age (\n                    <jats:italic>n<\/jats:italic>\n                    \u202f=\u202f5 per group) received a single oral dose of 20\u202fmg of the Fc-fused antibody in milk replacer; control calves received milk replacer alone. Blood samples were collected over 28\u202fdays. Treated calves showed numerically higher serum antibody signals than controls throughout the observation period, with statistically significant differences at days 6 and 10. An assay targeting the Fc region provided numerically greater group separation than a general antibody detection format. These results provide proof-of-principle that a single oral dose of an Fc-fused single-domain antibody was systemically detectable for up to 28\u202fdays in neonatal calves, supporting the potential for extended dosing intervals compared with conventional oral immunoglobulin strategies.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1827924","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:52:30Z","timestamp":1777441950000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Single oral dose of bovine Fc-fused single-domain antibody enables multi-week serum detectability in neonatal calves"],"prefix":"10.3389","volume":"13","author":[{"given":"Songyi","family":"Yang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University","place":["Chuncheon, Republic of Korea"]},{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaerin","family":"Kim","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeongsu","family":"Moon","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanearl","family":"Kim","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seonggyu","family":"Hong","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iqbal Maulana","family":"Taufik","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Kim","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soohyun","family":"Park","sequence":"additional","affiliation":[{"name":"ADbiotech Co., Ltd.","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeonghee","family":"Han","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University","place":["Chuncheon, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungjae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Health, Kyungbok University","place":["Namyangju, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/S0749-0720(15)30001-3","article-title":"Bovine neonatal immunology","volume":"17","author":"Barrington","year":"2001","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"672","DOI":"10.3168\/jds.S0022-0302(80)82989-4","article-title":"Absorption of colostral immunoglobulins in newborn calves","volume":"63","author":"Bush","year":"1980","journal-title":"J Dairy Sci"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1007\/s11250-006-4349-9","article-title":"Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998\u20132002","volume":"38","author":"Alfieri","year":"2006","journal-title":"Trop Anim Health Prod"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"442","DOI":"10.3390\/nu3040442","article-title":"Perspectives on immunoglobulins in colostrum and milk","volume":"3","author":"Hurley","year":"2011","journal-title":"Nutrients"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"681","DOI":"10.3168\/jds.S0022-0302(80)82990-0","article-title":"Immunoglobulin absorption in calf neonates with special considerations of stress","volume":"63","author":"Stott","year":"1980","journal-title":"J Dairy Sci"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"87","DOI":"10.12718\/AARS.2024.35.3.87","article-title":"The effect of oral administration of bovine rotavirus-specific single variable domain on calves infected with bovine rotavirus","volume":"35","author":"Yang","year":"2024","journal-title":"Ann Anim Resour Sci"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/j.tibtech.2023.01.015","article-title":"Orally delivered single-domain 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