{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:46:05Z","timestamp":1785818765589,"version":"3.56.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"\n Introduction<\/jats:title>\n This paper aimed to enhance the hatching of bovine in vitro produced embryos by exploring their optimal LAH perforation\/thinning pattern.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Ovaries were col-lected from an abattoir to obtain cumulus\u2013oocyte complexes. After in vitro fer-tilization, 4-cell, 8-cell, morula, and blastocyst-stage embryos were allocated to experimental groups, and their hatching outcomes were compared following four Laser-Assisted Hatching (LAH) treatments, which aim to facilitate embryo hatching from the zona pellucida and subsequent implantation into the endo-metrium by creating small pores or thinning the zona pellucida. The treatments included removal of\n 1<\/jats:sup>\n \/\n 4<\/jats:sub>\n of the zona pellucida, removal of\n 1<\/jats:sup>\n \/\n 3<\/jats:sub>\n of the zona pellucida, thinning of\n 1<\/jats:sup>\n \/\n 4<\/jats:sub>\n of the zona pellucida, and thinning of\n 1<\/jats:sup>\n \/\n 3<\/jats:sub>\n of the zona pellucida.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The results showed that: \u2460 The hatching rate of 4-cell embryos with\n 1<\/jats:sup>\n \/\n 4<\/jats:sub>\n zona removal was significantly higher than that of the control group (\n p<\/jats:italic>\n \u202f&lt;\u202f0.01); \u2461 the hatching rate of 8-cell embryos with removal of\n 1<\/jats:sup>\n \/\n 3<\/jats:sub>\n of the zona pellucida was significantly higher than that of the control group (\n p<\/jats:italic>\n \u202f&lt;\u202f0.01); \u2462 for morula-stage embryos, both\n 1<\/jats:sup>\n \/\n 3<\/jats:sub>\n zona removal and\n 1<\/jats:sup>\n \/\n 3<\/jats:sub>\n zona thinning resulted in significantly higher hatch-ing rates than the control (\n p<\/jats:italic>\n \u202f&lt;\u202f0.01); and \u2463 the hatching rate of blastocysts with removal of 1\/3 was significantly higher than that of the control group (\n p<\/jats:italic>\n \u202f&lt;\u202f0.01).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n In conclusion, the most effective LAH treatments were\n 1<\/jats:sup>\n \/\n 4<\/jats:sub>\n zona removal for 4-cell embryos and\n 1<\/jats:sup>\n \/\n 3<\/jats:sub>\n zona removal for 8-cell, morula, and blastocyst-stage embryos, all of which yielded improved hatching rates.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fvets.2026.1828209","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:26:38Z","timestamp":1785817598000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The effect of laser-assisted incubation on bovine in vitro embryos"],"prefix":"10.3389","volume":"13","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"Xinjiang Key Laboratory of Reproductive Regulation and Breeding for Ruminant Livestock, Xinjiang Academy of Animal Sciences","place":["Urumqi, China"]},{"name":"Institute of Biotechnology, Xinjiang Agricultural University","place":["Urumqi, China"]},{"name":"College of Animal Science, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Reproductive Regulation and Breeding for Ruminant Livestock, Xinjiang Academy of Animal Sciences","place":["Urumqi, China"]},{"name":"Institute of Biotechnology, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulmira","family":"Abudureyimu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Reproductive Regulation and Breeding for Ruminant Livestock, Xinjiang Academy of Animal Sciences","place":["Urumqi, China"]},{"name":"Institute of Biotechnology, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Dong","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Reproductive Regulation and Breeding for Ruminant Livestock, Xinjiang Academy of Animal Sciences","place":["Urumqi, China"]},{"name":"Institute of Biotechnology, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiapeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Reproductive Regulation and Breeding for Ruminant Livestock, Xinjiang Academy of Animal Sciences","place":["Urumqi, China"]},{"name":"Institute of Biotechnology, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqin","family":"Wang","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Reproductive Regulation and Breeding for Ruminant Livestock, Xinjiang Academy of Animal Sciences","place":["Urumqi, China"]},{"name":"Institute of Biotechnology, Xinjiang Agricultural University","place":["Urumqi, 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