{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:06:03Z","timestamp":1784732763148,"version":"3.55.0"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["RRF-2.3.1-21-2022-00001"],"award-info":[{"award-number":["RRF-2.3.1-21-2022-00001"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Poultry-associated enterococci are relevant to One Health surveillance, yet interpreting vancomycin-related genomic signals requires careful consideration of species biology and detection confidence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analyzed broth microdilution MIC data for 218 poultry-origin\n                      <jats:italic>Enterococcus<\/jats:italic>\n                      isolates from an archival strain bank in Hungary and paired these data with whole-genome sequencing (WGS) for 73 isolates with linked MIC records. Vancomycin MICs for isolates previously flagged as showing elevated vancomycin MICs and\/or phenotype\u2013genotype discordant profiles were re-tested in three parallel confirmatory broth microdilution measurements using single-colony subcultures, and final confirmed MIC values are reported.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the final dataset, no confirmed vancomycin MIC value exceeded 4 \u03bcg\/ml. Resistome screening of the 73 genomes (CARD-based output) yielded 1,448 resistance-associated annotations (181 unique gene calls; median 12 per genome) and did not detect canonical acquired van operons consistent with\n                      <jats:italic>vanA, vanB, vanD, vanM<\/jats:italic>\n                      , or\n                      <jats:italic>vanN<\/jats:italic>\n                      . van-associated signals were limited to\n                      <jats:italic>vanC<\/jats:italic>\n                      -like components,\n                      <jats:italic>vanG<\/jats:italic>\n                      -type regulatory elements (\n                      <jats:italic>vanRG<\/jats:italic>\n                      and\/or\n                      <jats:italic>vanSG<\/jats:italic>\n                      ), and a single\n                      <jats:italic>vanW<\/jats:italic>\n                      -annotated record, highlighting the need for species-aware interpretation and locus-level validation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results support a surveillance-grade workflow that anchors van-related genomic reporting to confirmatory phenotyping, genome-confirmed species assignment, and confidence-aware locus inspection to minimize false alarms and improve One Health interpretability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1831285","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:40:27Z","timestamp":1784731227000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Species-aware interpretation of vancomycin-related genomic signals in poultry-derived Enterococcus spp.: a confirmatory phenotyping and whole-genome sequencing framework for One Health surveillance"],"prefix":"10.3389","volume":"13","author":[{"given":"\u00c1d\u00e1m","family":"Kerek","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]},{"name":"National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gergely \u00c1lmos","family":"Tornyos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]},{"name":"National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levente Hunor","family":"Husz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]},{"name":"National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"Kaszab","sequence":"additional","affiliation":[{"name":"National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest","place":["Budapest, Hungary"]},{"name":"One Health Institute, University of Debrecen","place":["Debrecen, Hungary"]},{"name":"Department of Microbiology and Infectious Diseases, University of Veterinary 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