{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:06:23Z","timestamp":1779901583036,"version":"3.53.1"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Non-absorbed inorganic iron in the digestive tract can be directly utilized by microorganisms, particularly pathogens. This study aimed to evaluate the feasibility of substituting ferrous sulfate (FeSO\u2084) with lower doses of ferrous glycinate (Fe-Gly) in weaned piglets. A total of 30 weaned piglets were randomly assigned to three dietary treatments (\n                    <jats:italic>n<\/jats:italic>\n                    \u202f=\u202f10): a Control group, a Gly-Fe-50 group (50\u202fmg\/kg Fe-Gly), and a Gly-Fe-75 group (75\u202fmg\/kg Fe-Gly). Compared with 100\u202fmg\/kg FeSO\u2084, supplementation with 50 or 75\u202fmg\/kg Fe-Gly did not significantly affect growth performance parameters, indicating that Fe-Gly maintained comparable growth performance at a 25\u201350% lower inclusion level. Numerically, 50\u202fmg\/kg Fe-Gly showed higher ADFI and ADG, although these differences were not statistically significant. Fe-Gly supplementation was associated with improvements in intestinal barrier function in weaned piglets. Furthermore, Fe-Gly supplementation significantly elevated serum total iron-binding capacity (TIBC) (\n                    <jats:italic>p<\/jats:italic>\n                    \u202f&amp;lt;\u202f0.05), indicating enhanced iron transport efficiency. Gene expression and microbial sequencing analyses revealed that Fe-Gly upregulated antioxidant genes (\n                    <jats:italic>SLC7A11<\/jats:italic>\n                    ,\n                    <jats:italic>P62<\/jats:italic>\n                    ) in the jejunum. Additionally, it significantly augmented the proportion of beneficial microbes, such as\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    and\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    , while reducing the proportion of\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    and\n                    <jats:italic>Escherichia-Shigella<\/jats:italic>\n                    . In summary, because of its high bioavailability, 50\u202fmg\/kg Fe-Gly does more than meet the growth and metabolic demands of piglets; it also reduces iron accumulation in the hindgut lumen. This mechanism restricts iron availability for intestinal pathogens, inhibiting their proliferation and thereby improving intestinal health in piglets.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1834338","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T16:33:48Z","timestamp":1779899628000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary ferrous glycinate supplementation reshapes the gut microbiota and improves intestinal barrier function in weaned piglets by reducing luminal iron accumulation"],"prefix":"10.3389","volume":"13","author":[{"given":"Yilong","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University","place":["Chengdu, China"]},{"name":"College of Animal Science, Xichang University","place":["Xichang, Sichuan, China"]},{"name":"Hunan Debon Biotechnology Co., Ltd.","place":["Changning, Hunan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conghui","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yabin","family":"Wu","sequence":"additional","affiliation":[{"name":"Hunan Debon Biotechnology Co., Ltd.","place":["Changning, Hunan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianchao","family":"Gu","sequence":"additional","affiliation":[{"name":"Hunan Debon Biotechnology Co., Ltd.","place":["Changning, Hunan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Huang","sequence":"additional","affiliation":[{"name":"Tongwei Agricultural Development Co., Ltd.","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Tongwei Agricultural Development Co., Ltd.","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimin","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1042778","DOI":"10.3389\/fimmu.2022.1042778","article-title":"Weaning stress and intestinal health of piglets: a review","volume":"13","author":"Tang","year":"2022","journal-title":"Front 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