{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:50:11Z","timestamp":1782190211133,"version":"3.54.5"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Ticks are important vectors of bacteria belonging to the order Rickettsiales, several of which are causes zoonotic diseases.\n                    <jats:italic>Amblyomma triste<\/jats:italic>\n                    is the main vector of\n                    <jats:italic>Rickettsia parkeri<\/jats:italic>\n                    , the etiological agent responsible for most cases of human spotted fever rickettsiosis in Argentina. However, information on the circulation of rickettsial agents in protected areas of the La Plata River Hydrographic Basin, a region that includes highly urbanized environments and frequent contact between wildlife, domestic animals and humans, remains limited. The aim of this study was to investigate the presence of rickettsial bacteria in\n                    <jats:italic>A. triste<\/jats:italic>\n                    collected in protected areas of Buenos Aires Province, (La Plata River Hydrographic Basin, Argentina). Between September and December 2021, ticks were collected by vegetation flagging in four protected areas and analyzed individually. Polymerase chain reactions (PCR) for detecting the genus\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    was initially performed using a simple PCR to amplify a variablesized fragment of the 23S-5S\n                    <jats:italic>rRNA<\/jats:italic>\n                    intergenic spacer. An initial PCR was performed with primers for a 16S\n                    <jats:italic>rRNA<\/jats:italic>\n                    fragment for the Anaplasmataceae family. A total of 247 adult ticks were examined. Twelve specimens (4.9%) tested positive for\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    , and sequence analysis confirmed their identity as\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    . Positive ticks were detected in Ciervo de los Pantanos National Park and Campos del Tuy\u00fa National Park. In addition, two specimens yielded sequences corresponding to\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Midichloria sp. These findings confirm the presence of\n                    <jats:italic>R. parkeri<\/jats:italic>\n                    in\n                    <jats:italic>A. triste<\/jats:italic>\n                    populations from protected areas of the La Plata River Hydrographic Basin and provide the first evidence of\n                    <jats:italic>Ca.<\/jats:italic>\n                    Midichloria sp. in this tick species in Argentina. The results highlight the need for continued surveillance of tick-borne microorganisms in protected areas located near densely populated regions, where human exposure to infected ticks may occur.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1841468","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:32:21Z","timestamp":1782189141000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Rickettsia parkeri and Candidatus Midichloria sp. in Amblyomma triste ticks from protected areas of Buenos Aires Province (La Plata River Hydrographic Basin, Argentina)"],"prefix":"10.3389","volume":"13","author":[{"given":"Marina P.","family":"De Seta","sequence":"first","affiliation":[{"name":"Instituto de Zoonosis Luis Pasteur","place":["Buenos Aires City, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Krapp","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis Luis Pasteur","place":["Buenos Aires City, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula M.","family":"D\u00edaz P\u00e9rez","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis Luis Pasteur","place":["Buenos Aires City, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Gim\u00e9nez","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis Luis Pasteur","place":["Buenos Aires City, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Pastorino","sequence":"additional","affiliation":[{"name":"Departamento de Zoonosis Urbanas","place":["Avellaneda, Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Alonso","sequence":"additional","affiliation":[{"name":"Departamento de Zoonosis Urbanas","place":["Avellaneda, Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Vico","sequence":"additional","affiliation":[{"name":"Departamento de Zoonosis Urbanas","place":["Avellaneda, Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Zoonosis Urbanas","place":["Avellaneda, Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda N.","family":"De Salvo","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis Luis Pasteur","place":["Buenos Aires City, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel L.","family":"Cicuttin","sequence":"additional","affiliation":[{"name":"Instituto de Zoonosis Luis Pasteur","place":["Buenos Aires City, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"ref1","first-page":"375","volume-title":"Ticks of the Southern Cone of America: Diagnosis, Distribution and Hosts with Taxonomy, Ecology and Sanitary Importance","author":"Nava","year":"2017"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/j.1469-185X.2010.00151.x","article-title":"Rickettsial evolution in the light of comparative genomics","volume":"86","author":"Merhej","year":"2011","journal-title":"Biol Rev Camb Philos Soc"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","article-title":"Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila","volume":"51","author":"Dumler","year":"2001","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"3241","DOI":"10.1128\/AEM.03971-12","article-title":"\u201cCandidatus Midichloriaceae\u201d fam. 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