{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:53:48Z","timestamp":1792007628186,"version":"4.4.0"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Aquaculture is one of the world\u2019s major food-producing industries; however, intensive farming conditions expose fish to multiple stressors that impair immune function and tissue regeneration, increasing susceptibility to infectious diseases. Therefore, sustainable strategies to enhance wound healing and fish health are needed. This study evaluated the regenerative and immunomodulatory potential of\n                    <jats:italic>aloe vera<\/jats:italic>\n                    (\n                    <jats:italic>Aloe barbadensis<\/jats:italic>\n                    Miller) gel using zebrafish (\n                    <jats:italic>Danio rerio<\/jats:italic>\n                    ) larvae subjected to tail tip amputation.\n                    <jats:italic>Aloe vera<\/jats:italic>\n                    was administered by immersion and dietary supplementation before and\/or after injury. Regenerative and immune responses were assessed by quantifying leukocyte recruitment, analyzing the expression of genes involved in inflammation, oxidative stress, and tissue regeneration, and evaluating larval growth and skin mucus-producing cells.\n                    <jats:italic>Aloe vera<\/jats:italic>\n                    enhanced leukocyte recruitment and significantly improved tissue regeneration following injury. These effects were accompanied by modulation of innate immune markers (\n                    <jats:italic>il1b, mpx, mpeg1.1,<\/jats:italic>\n                    and\n                    <jats:italic>cox-2a<\/jats:italic>\n                    ) and antioxidant defense genes (\n                    <jats:italic>sod1, sod2,<\/jats:italic>\n                    and\n                    <jats:italic>gpx1a<\/jats:italic>\n                    ). Dietary supplementation with\n                    <jats:italic>aloe vera<\/jats:italic>\n                    also alleviated the detrimental effects of an inflammatory plant protein-based diet on regeneration, promoted larval growth, and increased both the number and size of skin goblet cells. Collectively, these findings demonstrate that\n                    <jats:italic>aloe vera<\/jats:italic>\n                    enhances immune function, antioxidant responses, tissue repair, and mucosal protection, supporting its potential as a natural functional additive to improve fish health and welfare in aquaculture.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1852633","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T14:13:02Z","timestamp":1786543982000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Aloe vera (Aloe barbadensis miller) gel extract enhances the immune response and tissue regeneration in the zebrafish larvae model"],"prefix":"10.3389","volume":"13","author":[{"given":"Javiera F.","family":"De la Paz Montt","sequence":"first","affiliation":[{"name":"Laboratorio de Embriotoxicolog\u00eda e Interacci\u00f3n Desarrollo Ambiente (LEIDA), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]},{"name":"Danio Biotechnologies SpA","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio","family":"Ya\u00f1ez-Bailey","sequence":"additional","affiliation":[{"name":"Laboratorio de Embriotoxicolog\u00eda e Interacci\u00f3n Desarrollo Ambiente (LEIDA), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]},{"name":"Laboratorio de Toxicolog\u00eda Acu\u00e1tica, Departamento de Oceanograf\u00eda, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Zambrano","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Acu\u00e1tica, Departamento de Oceanograf\u00eda, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila","family":"Garc\u00eda-Castro","sequence":"additional","affiliation":[{"name":"Laboratorio de Embriotoxicolog\u00eda e Interacci\u00f3n Desarrollo Ambiente (LEIDA), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Cruzat","sequence":"additional","affiliation":[{"name":"Laboratorio de Detecci\u00f3n Molecular de Microalgas T\u00f3xicas (LDMMTx), Departamento de Oceanograf\u00eda, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Llanos-Rivera","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Acu\u00e1tica, Departamento de Oceanograf\u00eda, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepci\u00f3n","place":["Concepci\u00f3n, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Fehrmann-Cartes","sequence":"additional","affiliation":[{"name":"N\u00facleo de Investigaci\u00f3n en Producci\u00f3n y Salud de Especies Acu\u00e1ticas (NIP-SEA), Facultad de Medicina Veterinaria y Agronom\u00eda, Universidad de Las Am\u00e9ricas","place":["Concepci\u00f3n, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref1","volume-title":"State of World Fisheries and Aquaculture 2022: Towards Blue Transformation","year":"2022"},{"key":"ref2","first-page":"121","article-title":"Variaci\u00f3n de la intensidad de infestaci\u00f3n con formas infectantes del isopodo Ceratothoa gaudichaudii en salmones de cultivo en el sur de Chile","volume":"1","author":"Sievers","year":"1997","journal-title":"Arch Med Vet"},{"key":"ref3","first-page":"241","volume-title":"Native Sea Lice (Copepoda: Caligidae) Infestation of Salmonids Reared in Netpen Systems in Southern Chile","author":"Carvajal","year":"1998"},{"key":"ref4","volume-title":"Informe de Seguimiento Programa Sanitario Especifico de Vigilancia Control Caligidosis. 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