{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:26:07Z","timestamp":1787199967121,"version":"build-2736575974"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Animal welfare is increasingly recognized as a key determinant of dairy herd health, yet its relationship with the presence of mastitis pathogens at the herd level remains insufficiently explored. This study aimed to evaluate the association between animal welfare indicators and the presence of major mastitis pathogens in bulk tank milk (BTM) from dairy cattle herds in the Aosta Valley, Italy, while also characterizing microbial profiles using bacteriological culture and polymerase chain reaction (PCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A broad microbiological screening was performed on unique BTM samples from 490 individual farms. Concurrently, a subset of 355 small- and medium-scale dairy cattle farms underwent detailed animal welfare assessments using the standardized ClassyFarm protocol. Data were collected by experienced assessors in northern Italy in 2022, with each farm visited once.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The median lactating herd size was 33 cows (IQR: 57\u201318) and included Aosta Red Pied and Aosta Black Pied cattle breeds. All farms were tie-stall in the Aosta Valley, where herds are grazed outdoors for most of the year and kept indoors in tied-stalls during winter. BC identified at least one pathogen in 73.5% of samples, with most positive samples exhibiting low diversity, predominantly single (48.6%) or dual-species (41.1%) profiles.\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      (\n                      <jats:italic>N<\/jats:italic>\n                      \u202f=\u202f281) and\n                      <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                      (\n                      <jats:italic>N<\/jats:italic>\n                      \u202f=\u202f148) were the most frequently isolated pathogens. In contrast, PCR revealed prevalence of polymicrobial contamination, with the majority of samples harboring four or more bacterial species and 44.4% containing \u22655 species. Logistic regression models built using the binary PCR outcomes for the 355-farm subset demonstrated consistent negative associations between animal welfare indicators and the presence of both\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                      and\n                      <jats:italic>S. aureus<\/jats:italic>\n                      . Higher total animal welfare scores were associated with reduced odds of detection (\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                      : OR = 0.943,\n                      <jats:italic>p<\/jats:italic>\n                      \u2264 0.001;\n                      <jats:italic>S. aureus<\/jats:italic>\n                      : OR = 0.948,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.005), as were animal-based measures, farm management, and housing and equipment scores.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings provide evidence that improved animal welfare is associated with a lower likelihood of major mastitis pathogens in BTM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1860743","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:16:20Z","timestamp":1787199380000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Association between animal welfare indicators and mastitis pathogens in bulk tank milk: the reality of small- and medium-scale dairy cattle farms in Italy"],"prefix":"10.3389","volume":"13","author":[{"given":"D\u00e1\u0161a","family":"Schleicherov\u00e1","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D\u2019Aosta","place":["Turin, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jatziri","family":"Mota-Gutierrez","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Veterinarie, Universit\u00e0 di Torino","place":["Grugliasco, Turin, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Forte","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Veterinarie, Universit\u00e0 di Torino","place":["Grugliasco, Turin, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ozella","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Veterinarie, Universit\u00e0 di Torino","place":["Grugliasco, Turin, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Fusi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna \u201cBruno Ubertini\u201d (IZSLER)","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Vevey","sequence":"additional","affiliation":[{"name":"Associazione Nazionale Bovini di Razza Valdostana","place":["Gressan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Bergagna","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D\u2019Aosta","place":["Turin, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref1","first-page":"177","article-title":"Scientific opinion on the welfare of dairy cows","volume-title":"EFSA J","author":"Nielsen","year":"2023"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1430","DOI":"10.3390\/ani10081430","article-title":"A review of welfare indicators of indoor-housed dairy cows as a basis for integrated automatic welfare assessment systems","volume":"10","author":"Leliveld","year":"2020","journal-title":"Animals (Basel)"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21014\/actaimeko.v13i1.1622","article-title":"Application of the 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