{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T17:13:49Z","timestamp":1783530829513,"version":"3.55.0"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    This study aimed to identify nematodes of the genus\n                    <jats:italic>Gongylonema<\/jats:italic>\n                    using morphological and molecular methods and to determine their occurrence in a group of non-human primates and anteaters. A single female nematode collected from\n                    <jats:italic>Macaca sylvanus<\/jats:italic>\n                    , together with carcasses of 29 non-human primates (belonging to 13 species) and two anteaters (representing one species) from Czech zoological gardens and private collections, were submitted between 2019 and 2025 to the State Veterinary Institute Prague for necropsies. Smears from the buccal cavity and tongue, as well as fecal samples, were collected by veterinarians at each facility and transported to the laboratory in tubes containing saline solution or in plastic bags, respectively. The samples were genetically characterized using the nuclear\n                    <jats:italic>18S<\/jats:italic>\n                    rRNA,\n                    <jats:italic>28S<\/jats:italic>\n                    rRNA genes, the internal transcribed spacer (ITS) region, and the mitochondrial cytochrome c oxidase subunit 1 gene (\n                    <jats:italic>cox1<\/jats:italic>\n                    ). Four non-human primates (two\n                    <jats:italic>Pithecia pithecia<\/jats:italic>\n                    , one\n                    <jats:italic>Saguinus labiatus<\/jats:italic>\n                    , and one\n                    <jats:italic>S. midas<\/jats:italic>\n                    ) and two anteaters (\n                    <jats:italic>Tamandua tetradactyla<\/jats:italic>\n                    ), from the same zoological garden, were positive for\n                    <jats:italic>Gongylonema (G.) primatum<\/jats:italic>\n                    sp. nov. The single female specimen from\n                    <jats:italic>M. sylvanus<\/jats:italic>\n                    was identified as\n                    <jats:italic>G. pulchrum<\/jats:italic>\n                    . The new species morphologically differed from its congeners in the body length of adult males, the left spicule length, the gubernaculum length, the protruded vulvar lips, and the number of precloacal papillae. Molecularly, the\n                    <jats:italic>18S<\/jats:italic>\n                    and\n                    <jats:italic>28S<\/jats:italic>\n                    rRNA genes were 99.88\u2212100% similar to those of\n                    <jats:italic>G. nepalensis<\/jats:italic>\n                    and\n                    <jats:italic>G. pulchrum<\/jats:italic>\n                    , whereas the ITS and\n                    <jats:italic>cox1<\/jats:italic>\n                    sequences showed lower similarity to those of both\n                    <jats:italic>Gongylonema<\/jats:italic>\n                    species.\n                    <jats:italic>Gongylonema primatum<\/jats:italic>\n                    sp. nov. was morphologically and molecularly different from its congeners and therefore represents a new species. This is the first record of the zoonotic\n                    <jats:italic>G. pulchrum<\/jats:italic>\n                    in\n                    <jats:italic>M. sylvanus<\/jats:italic>\n                    . Although\n                    <jats:italic>G. pulchrum<\/jats:italic>\n                    is zoonotic, the new species may also have zoonotic potential.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1862920","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:19:41Z","timestamp":1783527581000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["New insights into Gongylonema infections in exotic mammals: description of G. (G.) primatum sp. nov. and detection of zoonotic G. pulchrum"],"prefix":"10.3389","volume":"13","author":[{"given":"Ond\u0159ej","family":"M\u00e1ca","sequence":"first","affiliation":[{"name":"Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague","place":["Prague, Czechia"]},{"name":"Department of Pathology and Parasitology, State Veterinary Institute Prague","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gonz\u00e1lez-Sol\u00eds","sequence":"additional","affiliation":[{"name":"Department of Systematics and Aquatic Ecology, El Colegio de la Frontera Sur","place":["Chetumal, Quintana Roo, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1645\/17-3","article-title":"Gongylonema parasites of 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