{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T20:49:54Z","timestamp":1782506994268,"version":"3.54.5"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Goat brucellosis, caused by\n                    <jats:italic>Brucella melitensis<\/jats:italic>\n                    , is a globally important zoonosis, yet host metabolic responses across serological reactivity levels under natural field conditions remain poorly understood. In this exploratory cross-sectional study, 150 Saanen goats were stratified by two-step serology into seronegative (Group A), weakly seropositive (Group B; serum agglutination test (SAT) titer 1:50), and strongly seropositive (Group C; SAT titer \u22651:100) groups; eight animals per group (\n                    <jats:italic>n\u202f=<\/jats:italic>\n                    \u202f24 total) underwent broad-targeted liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) serum metabolomics, yielding 699 annotated metabolites across 39 categories. Metabolic separation intensified with serological reactivity and was most pronounced between Groups A and C. Amino acid metabolism pathways were consistently enriched across all pairwise comparisons, while pyruvate metabolism, the pentose phosphate pathway, and purine\/pyrimidine metabolism showed stage-associated enrichment only in comparisons involving Group C. D-erythrose 4-phosphate was the sole metabolite significantly altered in all three pairwise comparisons, with monotonically increasing abundance (area under the curve (AUC): A vs. B\u202f=\u202f0.766; A vs. C\u202f=\u202f1.000; B vs. C\u202f=\u202f0.891). Trimethylamine N-oxide exhibited a biphasic pattern, while symmetric dimethylarginine declined monotonically. Collectively, these results reveal a progressive, stratum-dependent reorganization of the serum metabolome in goat brucellosis and highlight D-erythrose 4-phosphate and several co-altered metabolites as priorities for validation in future studies.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1866534","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T20:39:35Z","timestamp":1782506375000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Stratum-specific serum metabolic reprogramming in Saanen goats with graded Brucella serological reactivity under natural exposure"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiaotong","family":"Zhao","sequence":"first","affiliation":[{"name":"Laboratory of Animal Science and Technology, College of Animal Science and Technology, Yunnan Agricultural University","place":["Kunming, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Chang","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Medicine, College of Veterinary Medicine, Yunnan Agricultural University","place":["Kunming, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiming","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Disease Prevention and Control Center of Shilin Yi Autonomous County","place":["Kunming, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhidong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chia Tai (Kunming) Co., Ltd.","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Hu","sequence":"additional","affiliation":[{"name":"Feed Supervision and Inspection Institute of Honghe Prefecture","place":["Mengzi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Xiang","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Science and Technology, College of Animal Science and Technology, Yunnan Agricultural University","place":["Kunming, Yunnan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e5692","DOI":"10.1371\/journal.pntd.0005692","article-title":"Caprine brucellosis: a historically neglected disease with significant impact on public health","volume":"11","author":"Rossetti","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/s12917-021-02743-x","article-title":"Prevalence, distribution and risk factors for brucellosis infection in goat farms in Ningxiang, 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