{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:02:58Z","timestamp":1789448578539,"version":"build-2803163510"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Slaughter by exsanguination via ventral neck incision (SEVNI), as performed in religious slaughter, has been criticized on the grounds that carotid occlusion may impair exsanguination and potentially delay loss of consciousness (LOC), leading to unnecessary pain and distress. The objective of this review is to evaluate the literature specifically addressing the prevalence of carotid occlusions (CO) in SEVNI of cattle and the available evidence for their potential consequences on cerebral perfusion, cortical function, and inferred LOC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A systematic search of PubMed, Google Scholar, Cochrane Library, Medline, and Web of Science identified studies assessing the prevalence and significance of CO in cattle after SEVNI. Non-cattle studies, reviews, commentaries and abstracts without full text were excluded. Studies were independently reviewed by co-authors to minimize risk of bias. Due to heterogeneity in methods and outcomes, results were synthesized narratively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fifteen studies met the inclusion criteria. ROBINS-I framework determined the overall risk of bias to be moderate. Across eligible prevalence cohorts, 13.8% of animals exhibited at least one reported carotid occlusive phenomenon. Conclusions suggesting delayed LOC in animals with CO are primarily inferential and rely on indirect evidence from a small number of studies with methodological limitations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Based on the available evidence, while CO do appear to occur in a subgroup of animals following SEVNI, there is insufficient evidence to demonstrate that they preserve cerebral perfusion or sustain cortical function at levels sufficient to delay LOC as inferred from available proxy measures. Available physiological and mathematical modeling data instead suggest that cortical perfusion would be expected to fall rapidly after bilateral carotid transection, but these data do not directly measure consciousness.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Systematic review registration<\/jats:title>\n                    <jats:p>\n                      <jats:ext-link>https:\/\/www.crd.york.ac.uk\/PROSPERO\/view\/CRD420261325822<\/jats:ext-link>\n                      , identifier PROSPERA; (registration number CRD420261325822).\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1868851","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:27:06Z","timestamp":1789446426000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Common carotid artery occlusion during slaughter without stunning in cattle: incidence and evidence for physiological significance\u2014a systematic review"],"prefix":"10.3389","volume":"13","author":[{"given":"Elie","family":"Girsowicz","sequence":"first","affiliation":[{"name":"Department of Surgery, McGill University","place":["Montreal, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ari Z.","family":"Zivotofsky","sequence":"additional","affiliation":[{"name":"Gonda Brain Center, Bar Ilan University","place":["Ramat Gan, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Rosen","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, Imperial College","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob R.","family":"Hascalovici","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine","place":["Bronx, NY, United States"]},{"name":"Hackensack University School of Medicine","place":["Nutley, NJ, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"ref1","volume-title":"Recommended Animal Handling Guidelines & Audit Guide: a Systematic Approach to Animal Welfare","author":"Grandin","year":"2017"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1080\/00480169.2014.964345","article-title":"A scientific comment on the welfare of domesticated ruminants slaughtered without stunning","volume":"63","author":"Johnson","year":"2015","journal-title":"N Z Vet J"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.meatsci.2016.03.011","article-title":"Consciousness, unconsciousness and death in the context of slaughter. Part I. Neurobiological mechanisms underlying stunning and killing","volume":"118","author":"Terlouw","year":"2016","journal-title":"Meat Sci"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.meatsci.2016.03.010","article-title":"Consciousness, unconsciousness and death in the context of slaughter. Part II. Evaluation methods","volume":"118","author":"Terlouw","year":"2016","journal-title":"Meat Sci"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.18174\/388582","volume-title":"Assessing Unconsciousness in Livestock at Slaughter","author":"Verhoeven","year":"2016"},{"key":"ref6","volume-title":"Report on Good and Adverse Practices - Animal Welfare Concerns in Relation to Slaughter Practices from the Viewpoint of Veterinary Sciences","author":"Von Holleben","year":"2010"},{"key":"ref7","volume-title":"Guidelines for the Humane Slaughter of Animals","year":"2024"},{"key":"ref8","volume-title":"Guidelines for ritual slaughter of food animals without pre-slaughter stunning [Online]","year":"2021"},{"key":"ref9","volume-title":"Meat Industry Recommended Animal Handling Guidelines","author":"Grandin","year":"2026"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"6275","DOI":"10.2903\/j.efsa.2020.6275","article-title":"Welfare of cattle at slaughter","volume":"18","author":"Nielsen","year":"2020","journal-title":"EFSA J"},{"key":"ref11","volume-title":"World Organization For Animal Health, Terrestrial Animal Health Code","year":"2021"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1007\/s10806-011-9324-4","article-title":"Government regulations of shechita (Jewish religious slaughter) in the twenty-first century: are they ethical?","volume":"25","author":"Zivotofsky","year":"2012","journal-title":"J Agric Environ Ethics"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2460\/ajvr.26.04.0174","article-title":"Quantitative EEG as a tool for evaluating pain-related neurophysiologic responses in cattle: a systematic review","author":"Freilich","year":"2026","journal-title":"Am J Vet Res"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1080\/00480169.2009.36883","article-title":"Components of electroencephalographic responses to slaughter in halothane-anaesthetised calves: effects of cutting neck tissues compared with major blood vessels","volume":"57","author":"Gibson","year":"2009","journal-title":"N Z Vet J"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1080\/00480169.2009.36882","article-title":"Electroencephalographic responses of halothane-anaesthetised calves to slaughter by ventral-neck incision without prior stunning","volume":"57","author":"Gibson","year":"2009","journal-title":"N Z Vet J"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0034-5288(18)31909-X","article-title":"Differences in behaviour between sheep and cattle during slaughter","volume":"37","author":"Blackmore","year":"1984","journal-title":"Res Vet Sci"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0309-1740(82)90040-7","article-title":"Electroencephalographic studies of stunning and slaughter of sheep and calves - part 2: the onset of permanent insensibility in calves during slaughter","volume":"6","author":"Newhook","year":"1982","journal-title":"Meat Sci"},{"key":"ref18","volume-title":"Shechita. 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