{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T05:02:02Z","timestamp":1784523722696,"version":"3.55.0"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Porcine epidemic diarrhea (PED) is a highly contagious enteric disease caused by the porcine epidemic diarrhea virus (PEDV), with mortality rates exceeding 80% in piglets. While the pathogenesis of PEDV is well-documented, its relationship with mitophagy remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used transmission electron microscopy (TEM) to observe ultrastructural changes in PEDV-infected Vero cells. Western blotting and biochemical assays were employed to analyze mitophagy-related markers (LC3-II, PINK1, Parkin, Beclin1) and oxidative stress indicators (ROS, MDA, antioxidant enzymes). The role of the PINK1\/Parkin pathway was further validated through siRNA knockdown and overexpression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>PEDV infection was found to trigger PINK1\/Parkin-dependent mitophagy, evidenced by the accumulation of damaged mitochondria and upregulation of mitophagy markers. This process was driven by ROS-induced oxidative stress, as ROS scavenging significantly attenuated mitophagy. Furthermore, we demonstrated that mitophagy facilitates PEDV replication, with Parkin expression acting as a critical regulator of this viral-induced autophagic pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>This study reveals that PEDV exploits PINK1\/Parkin-mediated mitophagy via ROS accumulation to promote viral replication. These findings provide a scientific basis for understanding PEDV pathogenesis and offer a technical reference for optimizing high-titer virus production in vaccine development.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1892510","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T04:20:43Z","timestamp":1784521243000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Porcine epidemic diarrhea virus induces Vero cell mitophagy by regulating ROS-mediated PINK1\/Parkin signal pathway"],"prefix":"10.3389","volume":"13","author":[{"given":"Yuhe","family":"Jin","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Han","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoran","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanfan","family":"Qiao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenli","family":"Bai","sequence":"additional","affiliation":[{"name":"Tongliao Animal Disease Prevention and Control Center","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Inner Mongolia Minzu University","place":["Tongliao, China"]},{"name":"Tongliao Academy of Agriculture and Animal Husbandry Sciences","place":["Tongliao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1738","DOI":"10.3390\/microorganisms13081738","article-title":"Multi-strain probiotic regulates the intestinal mucosal immunity and enhances the protection of piglets against porcine epidemic diarrhea virus challenge","volume":"13","author":"Wang","year":"2025","journal-title":"Microorganisms"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/BF01317606","article-title":"A new coronavirus-like particle associated with diarrhea in swine","volume":"58","author":"Pensaert","year":"1978","journal-title":"Arch Virol"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"198045","DOI":"10.1016\/j.virusres.2020.198045","article-title":"Porcine epidemic diarrhea virus (PEDV): an update on etiology, transmission, pathogenesis, and prevention and control","volume":"286","author":"Jung","year":"2020","journal-title":"Virus Res"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"161","DOI":"10.3201\/eid1801.111259","article-title":"Outbreak of porcine epidemic diarrhea in suckling piglets, China","volume":"18","author":"Sun","year":"2012","journal-title":"Emerg Infect Dis"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.virusres.2016.05.023","article-title":"Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains","volume":"226","author":"Lin","year":"2016","journal-title":"Virus Res"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"685","DOI":"10.3201\/eid2004.131685","article-title":"Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs","volume":"20","author":"Jung","year":"2014","journal-title":"Emerg. 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