{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T05:07:52Z","timestamp":1789967272003,"version":"4.0.1"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Antimicrobial-resistant (AMR) Enterobacterales, including extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing organisms, represent an important One Health concern involving humans, animals, and shared environments. This cross-sectional study evaluated survey-based risk factors for oral and oropharyngeal Enterobacterales carriage in humans and companion animals and characterized selected isolates using whole-genome sequencing (WGS). Human and companion animal samples were collected from participants presenting to the Mississippi State University College of Veterinary Medicine. A 27-item questionnaire assessed pet-related exposures, household antimicrobial use, occupational exposures, and human-animal interactions. Among 255 humans, 13.7% were positive for Enterobacterales and 7.5% for\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp. Among 197 dogs, 47.2% were positive for Enterobacterales, including 30.5% positive for\n                    <jats:italic>E. coli<\/jats:italic>\n                    and 8.1% positive for\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp. Only one of 18 cats was positive for Enterobacterales. In humans, veterinary occupational exposure demonstrated a nonsignificant positive trend toward\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp. carriage (OR\u202f=\u202f2.537, 95% CI: 0.963\u20136.688,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f=\u202f0.060). In dogs, antibiotic administration within the previous 12\u202fmonths was associated with increased odds of\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp. carriage (OR\u202f=\u202f3.850, 95% CI: 1.046\u201314.173,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f=\u202f0.043) and\n                    <jats:italic>E. coli<\/jats:italic>\n                    carriage (OR\u202f=\u202f3.049, 95% CI: 1.517\u20136.129,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f=\u202f0.002). Recent hospitalization within the previous 3\u202fmonths was also associated with increased odds of canine\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp. carriage (OR\u202f=\u202f4.134, 95% CI: 1.400\u201312.213,\n                    <jats:italic>p<\/jats:italic>\n                    \u202f=\u202f0.010). Whole-genome sequencing of 36 Enterobacterales isolates revealed substantial genomic diversity and identified multiple clinically important resistance determinants, including CTX-M-15 and NDM-5. A previously unassigned\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    sequence type identified in both a human and canine isolate was subsequently assigned ST9790 through the Institut Pasteur Klebsiella MLST database. No shared bacterial species or sequence types were identified within matched human-animal pairs. These findings demonstrate associations between antimicrobial exposure and Enterobacterales carriage in companion animals and highlight the presence of clinically important antimicrobial resistance determinants in both humans and dogs. This study further supports the importance of continued One Health surveillance and antimicrobial stewardship efforts at the human-animal interface.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1900404","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:28:31Z","timestamp":1789964911000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Survey-based risk factors and genomic characterization of oral and oropharyngeal Enterobacterales carriage in humans and companion animals: a One Health study"],"prefix":"10.3389","volume":"13","author":[{"given":"W. Cooper","family":"Brookshire","sequence":"first","affiliation":[{"name":"Mississippi State University","place":["Starkville, MS, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Taylor","family":"Hellmann","sequence":"additional","affiliation":[{"name":"The University of Mississippi Medical Center","place":["Jackson, MS, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Ashley","family":"Robinson","sequence":"additional","affiliation":[{"name":"The University of Mississippi Medical Center","place":["Jackson, MS, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keun Seok","family":"Seo","sequence":"additional","affiliation":[{"name":"Mississippi State University","place":["Starkville, MS, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"ref1","year":"2016"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1007\/s42770-021-00667-0","article-title":"Klebsiella-induced infections in domestic species: a case-series study in 697 animals (1997\u20132019)","volume":"53","author":"Ribeiro","year":"2022","journal-title":"Braz J Microbiol"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1128\/CMR.11.4.589","article-title":"Klebsiella spp. as nosocomial pathogens: epidemiology, 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Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1900404\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:28:33Z","timestamp":1789964913000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1900404\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"references-count":45,"alternative-id":["10.3389\/fvets.2026.1900404"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2026.1900404","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]},"article-number":"1900404"}}