{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:04:05Z","timestamp":1784783045019,"version":"3.55.0"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Leptospirosis is a widespread zoonotic disease caused by pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp., affecting humans, domestic animals, and wildlife. Wild mammals are recognized as potential maintenance hosts for pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp., and some species may act as asymptomatic reservoirs, representing a potential public health risk, especially in areas with increasing human-wildlife interaction. This cross-sectional study investigated the occurrence of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. in wild mammals from Para\u00edba, Brazil, sampled between June 2022 and June 2023 using serological and molecular approaches. Blood samples from 60 animals were analyzed by the microscopic agglutination test (MAT), while tissue samples from 29 animals were evaluated by polymerase chain reaction (PCR) targeting the\n                    <jats:italic>lipL32<\/jats:italic>\n                    gene, a marker specific for pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. Seroreactivity was detected in 9\/60 animals (15.0%), with reactions against the serogroups Pomona, Hebdomadis, Grippotyphosa, Shermani, Patoc, and Pyrogenes. Pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    DNA was detected in 6\/29 animals (20.7%), with positive PCR results obtained from multiple tissues, including the kidneys, bladder, liver, brain, and reproductive organs. These findings demonstrate exposure to and infection by pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. in wild mammals from Para\u00edba and reinforce the importance of wildlife surveillance for a better understanding of the epidemiology of leptospirosis.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1904553","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T04:31:28Z","timestamp":1784781088000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and serological detection of Leptospira spp. in wildlife from Para\u00edba, Brazil"],"prefix":"10.3389","volume":"13","author":[{"given":"Ividy","family":"Bison","sequence":"first","affiliation":[{"name":"Postgraduate Program in Animal Science, Federal University of Para\u00edba","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audisio A.","family":"Costa Filho","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, Federal University of Para\u00edba","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenison F.","family":"Dias","sequence":"additional","affiliation":[{"name":"Brazilian Institute of the Environment and Renewable Natural Resources","place":["Jo\u00e3o Pessoa, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R\u00f4mulo S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, Federal University of Para\u00edba","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeann L.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, Federal University of Para\u00edba","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luana Cristiny R.","family":"Silva","sequence":"additional","affiliation":[{"name":"Academic Unit of Veterinary Medicine, Federal University of Campina Grande","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Santos De","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Academic Unit of Veterinary Medicine, Federal University of Campina Grande","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta N.","family":"Parentoni","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, Federal University of Para\u00edba","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Rannier Ribeiro Antonino","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, Karolinska Institutet","place":["Stockholm, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur Willian De Lima","family":"Brasil","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, Federal University of Para\u00edba","place":["Para\u00edba, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2722","DOI":"10.3390\/ani11092722","article-title":"Exposure of free-ranging wild animals to zoonotic Leptospira interrogans sensu stricto in Slovenia","volume":"11","author":"\u017dele-Vengu\u0161t","year":"2021","journal-title":"Animals"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.3390\/pathogens11111376","article-title":"An overview of 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Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1904553\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T04:31:33Z","timestamp":1784781093000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1904553\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,23]]},"references-count":28,"alternative-id":["10.3389\/fvets.2026.1904553"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2026.1904553","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,23]]},"article-number":"1904553"}}