{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:04:53Z","timestamp":1789621493923,"version":"build-2803163510"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study was designed to evaluate the effects of supplementing dairy cow diets with lysophosphatidylcholine (LPL) on production performance, serum biochemical parameters, and rumen fermentation profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 20 Holstein dairy cows in the early lactation stage with comparable body conditions (DIM = 73 \u00b1 26 d; milk yield = 41 \u00b1 10 kg; average parity = 2.6 \u00b1 1.1) were selected and randomly assigned to two groups:the control group (CON), which received a basal diet only, and the experimental group (LPL), which was supplemented with 10 g\/day of lysophosphatidylcholine (LPL) in addition to the basal diet. The experimental period lasted for 60 days. At the conclusion of the trial, samples were collected for the assessment of apparent digestibility, production performance, serum biochemical parameters, and rumen fermentation profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results demonstrated that LPL supplementation significantly increased milk yield, milk fat percentage, and milk protein concentration. It also improved the apparent digestibility of crude fat and enhanced rumen fermentation by increasing propionate and microbial crude protein levels while decreasing ammonia nitrogen concentration. Blood analysis revealed elevated levels of glucose and non-esterified fatty acids (NEFA), along with reduced concentrations of triglycerides (TG) and blood urea nitrogen (BUN). Although LPL had minimal impact on overall rumen microbial diversity, it altered the relative abundance of specific bacterial species.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Dietary supplementation with 10 g\/day LPL improved milk yield, milk fat percentage, milk protein percentage, and and apparent EE digestibility in lactating dairy cows. LPL also altered selected serum biochemical parameters related to glucose and lipid metabolism, while showing limited effects on overall rumen bacterial diversity and community structure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1912675","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:47:27Z","timestamp":1789620447000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects oflysophospholipids supplementation on production performance, serum biochemistry, rumen fermentation in lactating dairy cows"],"prefix":"10.3389","volume":"13","author":[{"given":"Ruoran","family":"Tao","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dian","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center of Technology Innovation for Dairy","place":["Hohhot, China"]},{"name":"Inner Mongolia Youran Dairy Group Limited","place":["Hohhot, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maocheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghao","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiyun","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Zhuo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbo","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"265S","DOI":"10.1093\/ajcn\/82.1.265S","article-title":"The economics of obesity: dietary energy density and energy cost","volume":"82","author":"Drewnowski","year":"2005","journal-title":"Am J Clin Nutr."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1093\/jn\/134.6.1487","article-title":"Dietary antibiotic growth promoters enhance the bioavailability of \u03b1-tocopheryl acetate in broilers by altering lipid absorption","volume":"134","author":"Knarreborg","year":"2004","journal-title":"J Nutr."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3168\/jds.S0022-0302(80)82881-5","article-title":"Fat in lactation rations: review","volume":"63","author":"Palmquist","year":"1980","journal-title":"J Dairy Sci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"3851","DOI":"10.3168\/jds.S0022-0302(93)77727-9","article-title":"Lipid metabolism in the rumen","volume":"76","author":"Jenkins","year":"1993","journal-title":"J Dairy Sci."},{"key":"B5","volume-title":"Nutrient Requirements of Dairy Cattle","year":"2021"},{"key":"B6","doi-asserted-by":"publisher","first-page":"3473","DOI":"10.2527\/1992.70113473x","article-title":"Effects of exogenous emulsifiers and fat sources on nutrient digestibility, serum lipids, and growth performance in weanling pigs","volume":"70","author":"Jones","year":"1992","journal-title":"J Anim Sci."},{"key":"B7","doi-asserted-by":"publisher","first-page":"3110","DOI":"10.3168\/jds.2018-15777","article-title":"Effects oflysophospholipids on short- term production, nitrogen utilization, and rumen fermentation and bacterial population in lactating dairy cows","volume":"102","author":"Lee","year":"2019","journal-title":"J Dairy Sci."},{"key":"B8","doi-asserted-by":"publisher","first-page":"5457","DOI":"10.3168\/jds.2020-19406","article-title":"Supplementation of lysolecithin in milk replacer for Holstein dairy calves: effects on growth performance, health, and metabolites","volume":"104","author":"Reis","year":"2021","journal-title":"J Dairy Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"108415","DOI":"10.1016\/j.pharmthera.2023.108415","article-title":"Lysophospholipids and their producing enzymes: their pathological roles and potential as pathological biomarkers","volume":"246","author":"Yaginuma","year":"2023","journal-title":"Pharmacol Ther."},{"key":"B10","doi-asserted-by":"publisher","first-page":"805","DOI":"10.3390\/ani9100805","article-title":"Dietary supplementation of lysophospholipids affects feed digestion in lambs","volume":"9","author":"Huo","year":"2019","journal-title":"Animals."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1111\/jpn.12935","article-title":"Effects of dietary supplemental lysophospholipids and vitamin C on performance, antioxidant enzymes, lipid peroxidation, thyroid hormones and serum metabolites of broiler chickens reared under thermoneutral and high ambient temperature","volume":"102","author":"Zangeneh","year":"2018","journal-title":"J Anim Physiol Anim Nutr."},{"key":"B12","doi-asserted-by":"publisher","first-page":"2601","DOI":"10.2527\/2004.8292601x","article-title":"Effects of emulsification, fat encapsulation, and pelleting on weanling pig performance and nutrient digestibility","volume":"82","author":"Xing","year":"2004","journal-title":"J Anim Sci."},{"key":"B13","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1093\/jn\/115.5.675","article-title":"Digestion and absorption of lipids in poultry","volume":"115","author":"Krogdahl","year":"1985","journal-title":"J Nutr."},{"key":"B14","doi-asserted-by":"publisher","first-page":"593","DOI":"10.3382\/ps\/pew269","article-title":"Effects oflysophospholipid supplementation to lower nutrient diets on growth performance, intestinal morphology, and blood metabolites in broiler n chickens","volume":"96","author":"Boontiam","year":"2017","journal-title":"Poult Sci."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1557","DOI":"10.1071\/AN18495","article-title":"Dietary addition oflysophospholipids and vitamin C affects growth performance, serum metabolites, antioxidant capacity and carcass characteristics of broiler chickens reared under low ambient temperature","volume":"60","author":"Zangeneh","year":"2020","journal-title":"Anim Prod Sci."},{"key":"B16","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1080\/00071668.2015.1067878","article-title":"Influence of high dose of phytase and an emulsifier on performance, apparent metabolisable energy and nitrogen retention in broilers fed on diets containing soy oil or tallow","volume":"56","author":"Zaefarian","year":"2015","journal-title":"Br Poult Sci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1080\/1828051X.2016.1192965","article-title":"Effect of dietary supplementation oflysophospholipids on productive performance, nutrient digestibility and carcass quality traits of broiler chickens","volume":"15","author":"Zampiga","year":"2016","journal-title":"Ital J Anim Sci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.3382\/ps\/pew330","article-title":"Effects of dietary fat source and supplemental lysophosphatidylcholine on performance, immune responses, and ileal nutrient digestibility in broilers fed corn\/soybean meal- or corn\/wheat\/soybean meal-based diets","volume":"96","author":"Allahyari-Bake","year":"2017","journal-title":"Poult Sci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"215","DOI":"10.3390\/ani10020215","article-title":"Effects of the supplementation of lysophospholipids through pelleted total mixed rations on blood biochemical parameters and milk production and composition of mid-lactation dairy cows","volume":"10","author":"He","year":"2020","journal-title":"Animals."},{"key":"B20","volume-title":"Nutrient Requirements of Dairy Cattle","year":"2001"},{"key":"B21","volume-title":"Official Methods of Analysis of AOAC International","year":"2023"},{"key":"B22","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","article-title":"Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition","volume":"74","author":"Van Soest","year":"1991","journal-title":"J Dairy Sci."},{"key":"B23","doi-asserted-by":"publisher","first-page":"282","DOI":"10.2527\/jas1977.442282x","article-title":"Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies","volume":"44","author":"Van Keulen","year":"1977","journal-title":"J Anim Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"9042","DOI":"10.3168\/jds.2017-12976","article-title":"Short communication: effects oflysolecithin on milk fat synthesis and milk fatty acid profile of cows fed diets differing in fiber and unsaturated fatty acid concentration","volume":"100","author":"Rico","year":"2017","journal-title":"J Dairy Sci."},{"key":"B25","doi-asserted-by":"publisher","first-page":"536","DOI":"10.3168\/jds.S0022-0302(00)74913-7","article-title":"Effects of Tween 60 and Tween 80 on protease activity, thiol group reactivity, protein adsorption, and cellulose degradation by rumen microbial enzymes","volume":"83","author":"Kamande","year":"2000","journal-title":"J Dairy Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"529","DOI":"10.2527\/jas1989.672529x","article-title":"Influence of phospholipids on ruminal fermentation in vitro and on nutrient digestion and serum lipids in sheep","volume":"67","author":"Jenkins","year":"1989","journal-title":"J Anim Sci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.2527\/jas1987.6451526x","article-title":"Effect of fats and fatty acid combinations on ruminal fermentation in semi-continuous in vitro cultures","volume":"64","author":"Jenkins","year":"1987","journal-title":"J Anim Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"998","DOI":"10.56093\/ijans.v84i9.43680","article-title":"Effect of feeding rice bran lyso- phospholipids and rumen protected fat on feed intake, nutrient utilization and milk yield in crossbred cows","volume":"84","author":"Sontakke","year":"2014","journal-title":"Indian J Anim Sci."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1590","DOI":"10.5713\/ajas.19.0850","article-title":"Dietary lysophospholipids supplementation inhibited the activity of lipolytic bacteria in forage with high oil diet: an in vitro study","volume":"33","author":"Kim","year":"2020","journal-title":"Asian-Australas J Anim Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"144","DOI":"10.3390\/microorganisms10010144","article-title":"The temporal dynamics of rumen microbiota in early weaned lambs","volume":"10","author":"Wang","year":"2022","journal-title":"Microorganisms."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1965","DOI":"10.3390\/ani14131965","article-title":"Exploring the effect of gastrointestinal Prevotella on growth performance traits in livestock animals","volume":"14","author":"Kou","year":"2024","journal-title":"Animals."},{"key":"B32","doi-asserted-by":"publisher","first-page":"e0198225","DOI":"10.1371\/journal.pone.0198225","article-title":"Illumina sequencing analysis of the ruminal microbiota in high-yield and low-yield lactating dairy cows","volume":"13","author":"Tong","year":"2018","journal-title":"PLoS ONE."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1868","DOI":"10.1111\/jam.14873","article-title":"Effect of different glucogenic to lipogenic nutrient ratios on rumen fermentation and bacterial community in vitro","volume":"130","author":"Hua","year":"2021","journal-title":"J Appl Microbiol."}],"container-title":["Frontiers in Veterinary Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1912675\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:47:29Z","timestamp":1789620449000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fvets.2026.1912675\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":33,"alternative-id":["10.3389\/fvets.2026.1912675"],"URL":"https:\/\/doi.org\/10.3389\/fvets.2026.1912675","relation":{},"ISSN":["2297-1769"],"issn-type":[{"value":"2297-1769","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]},"article-number":"1912675"}}