{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T05:24:28Z","timestamp":1786944268696,"version":"build-2736575974"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    is a zoonotic pathogen infecting a wide range of domestic and wild animals. Although genomic data are available for several domestic hosts, isolates from wildlife remain underrepresented. Here, we performed whole-genome sequencing of nine\n                    <jats:italic>P. multocida<\/jats:italic>\n                    isolates recovered in Connecticut between 2022 and 2023 from cows, a deer, a rabbit, a cat, and a pheasant to characterize their genetic features and enhance our understanding of the host-associated diversity. All isolates except one feline strain shared a common genotype across multiple host species, being classified as capsular type A and LPS type L3. In contrast, the feline isolate lacked a detected capsule locus and was classified as LPS type L1. Bovine isolates exhibited higher antimicrobial resistance, including the only multidrug-resistant isolate, and exclusively harbored antimicrobial resistance (AMR) genes [\n                    <jats:italic>aph<\/jats:italic>\n                    (3\u2032)-Ia,\n                    <jats:italic>aph<\/jats:italic>\n                    (\n                    <jats:xref>6<\/jats:xref>\n                    )-Id,\n                    <jats:italic>sul2<\/jats:italic>\n                    , and\n                    <jats:italic>tet<\/jats:italic>\n                    (H)], whereas non-bovine isolates showed low resistance and no evidence of AMR determinants. Multilocus sequence typing revealed that all bovine isolates belonged to ST1 (Multi-Host scheme) and ST79 (RIRDC scheme), while non-bovine isolates displayed greater genetic diversity, including five novel sequence types. Virulence-associated genes were largely conserved across hosts. Core-genome SNP-based phylogenetic analysis revealed largely host-associated clustering, with bovine isolates grouping with bovine lineages from other countries and non-bovine isolates clustering with genetically diverse international lineages, including avian and human-associated strains. Collectively, these findings highlight host-linked patterns in AMR and genomic diversity of\n                    <jats:italic>P. multocida<\/jats:italic>\n                    and underscore the importance of integrated phenotypic and genomic surveillance across animal hosts.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1920717","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:33:06Z","timestamp":1786941186000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic diversity and novel sequence types of Pasteurella multocida from diverse animal hosts in Connecticut, United States, 2022\u20132023"],"prefix":"10.3389","volume":"13","author":[{"given":"Jillian","family":"Barron","sequence":"first","affiliation":[{"name":"Connecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut","place":["Storrs, CT, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina Francesca","family":"Soriano","sequence":"additional","affiliation":[{"name":"Connecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut","place":["Storrs, CT, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab H.","family":"Helal","sequence":"additional","affiliation":[{"name":"Connecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut","place":["Storrs, CT, United States"]},{"name":"Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut","place":["Storrs, CT, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Yeon","family":"Hyeon","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University","place":["Seoul, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo R.","family":"Risatti","sequence":"additional","affiliation":[{"name":"Connecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut","place":["Storrs, CT, United States"]},{"name":"Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut","place":["Storrs, CT, United 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