{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:08:10Z","timestamp":1789103290227,"version":"build-2803163510"},"reference-count":84,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The Cape hare (\n                      <jats:italic>L. capensis<\/jats:italic>\n                      ) is one of the most common game species in Algeria, although its populations have declined in recent years. This wild lagomorph may host ticks, which act as vectors for a range of zoonotic pathogens. The purpose of this study is to fill the gaps in current knowledge regarding the presence of ticks in Algerian cape hare species and better understand the potential implications for public health.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      During the 2023\u20132024 hunting seasons in the High Plateaus of Algeria, a total of 47 lagomorphs (44 Cape hares and 3 wild rabbits) were collected from 20 sites and examined for tick infestation. Ticks were identified using both morphological and molecular approaches. The presence of bacterial pathogens (\n                      <jats:italic>Borrelia<\/jats:italic>\n                      spp.,\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      spp.,\n                      <jats:italic>Anaplasma<\/jats:italic>\n                      spp.,\n                      <jats:italic>Coxiella<\/jats:italic>\n                      spp.,\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      spp.) and viral agents (Tick Borne Encephalites Virus, Crimean Congo Haemorragic Fever, West Nile Virus, Usutu virus, and phleboviruses) was assessed using quantitative real-time PCR on 56 ticks pools.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty-two hares (50%) but no rabbits were infested with ticks. A total of 165 ticks were collected, predominantly\n                      <jats:italic>Rhipicephalus sanguineus<\/jats:italic>\n                      sensu lato (94.5%), followed by\n                      <jats:italic>Ixodes hexagonus<\/jats:italic>\n                      (5.5%). Adult ticks were the most abundant life stage, followed by nymphs, while larvae were rarely observed. Seasonal analysis showed a peak infestation in September (100% prevalence, mean intensity 11.57\u202f\u00b1\u202f22.37 ticks\/animal), followed by a decline in October (40.9%, 2.27\u202f\u00b1\u202f3.77 ticks\/animal) and November (46.2%, 1.82\u202f\u00b1\u202f3.52 ticks\/animal). No ticks were detected in December. Molecular screening revealed seven zoonotic\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      species in\n                      <jats:italic>R. sanguineus<\/jats:italic>\n                      , with\n                      <jats:italic>R. aeschlimannii<\/jats:italic>\n                      being most prevalent (19.6%). Additionally,\n                      <jats:italic>Borrelia afzelii<\/jats:italic>\n                      (3.6%) and\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      sensu stricto (1.8%) were detected, with a total prevalence of 0.7% (4 positive pools). All ticks were negative for\n                      <jats:italic>Anaplasma<\/jats:italic>\n                      spp.,\n                      <jats:italic>Coxiella<\/jats:italic>\n                      spp.,\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      spp., and screened viruses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>R. sanguineus<\/jats:italic>\n                      is the predominant tick species infesting Cape hares in Algeria. The detection of multiple zoonotic pathogens highlights the potential role of this host in the epidemiology of tick-borne diseases and underscores the need for expanded surveillance in wild lagomorph populations within a One Health framework.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fvets.2026.1936427","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:35:55Z","timestamp":1789101355000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Epidemiological study on ticks and tick-borne pathogens in cape hare (Lepus capensis) in Algeria"],"prefix":"10.3389","volume":"13","author":[{"given":"Abderrazek","family":"Bendrimia","sequence":"first","affiliation":[{"name":"Laboratory EHPREC, Department of Veterinary Sciences, Faculty of Natural and Life Sciences, University Chadli Ben Djedid","place":["El Tarf, Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faouzi","family":"Matallah","sequence":"additional","affiliation":[{"name":"Laboratory EHPREC, Department of Veterinary Sciences, Faculty of Natural and Life Sciences, University Chadli Ben Djedid","place":["El Tarf, Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Defilippo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Rigamonti","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Prati","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Sampieri","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Cavadini","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Lavazza","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Trogu","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna","place":["Brescia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Besbaci","sequence":"additional","affiliation":[{"name":"LAB, Veterinary Institute, Saad Dahleb University","place":["Blida, Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofiane","family":"Bensefia","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, Faculty of Natural and Life Sciences, University Mohamed El Bachir El Ibrahimi","place":["Bordj Bou Arreridj, Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamza","family":"Khaled","sequence":"additional","affiliation":[{"name":"LAB, Veterinary Institute, Saad Dahleb University","place":["Blida, Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madjid","family":"Akkou","sequence":"additional","affiliation":[{"name":"LAB, Veterinary Institute, Saad Dahleb University","place":["Blida, 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bravo","author":"Rouco","year":"2006"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1111\/j.1365-2656.2007.01233.x","article-title":"Predation as a landscape effect: the trading off by prey species between predation risks and protection benefits","volume":"76","author":"M\u00f6nkk\u00f6nen","year":"2007","journal-title":"J Anim Ecol"},{"key":"ref5","volume-title":"Fauna d\u2019Italia: Acari Ixodida","author":"Manilla","year":"1998"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"96","DOI":"10.29261\/pakvetj\/2025.122","article-title":"The acaricidal and repellent efficacy of essential oils and their immunomodulatory effects against Hyalomma ticks: a review article","volume":"45","author":"Omar","year":"2025","journal-title":"Pak Vet J"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1093\/jme\/tjaa209","article-title":"Impact of land use changes and habitat fragmentation on the eco-epidemiology of tick-borne 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Vectors"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"e0006064","DOI":"10.1371\/journal.pntd.0006064","article-title":"Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria","volume":"11","author":"Lafri","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"362","DOI":"10.3390\/pathogens10030362","article-title":"High-throughput microfluidic real-time PCR for the detection of multiple microorganisms in ixodid cattle ticks in Northeast Algeria","volume":"10","author":"Boularias","year":"2021","journal-title":"Pathogens"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"2085","DOI":"10.1007\/s00436-020-06725-0","article-title":"Mitochondrial 16S rRNA gene analysis reveals occurrence of Rhipicephalus sanguineus sensu stricto from steppe and high plateaus regions","volume":"119","author":"Laatamna","year":"2020","journal-title":"Algeria"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.cimid.2011.11.007","article-title":"Rickettsiae in arthropods collected from the north African hedgehog (Atelerix algirus) and the desert hedgehog (Paraechinus aethiopicus) in Algeria","volume":"35","author":"Khaldi","year":"2012","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s13028-015-0132-0","article-title":"Health screening of free-ranging European brown hares (Lepus europaeus) on the German North Sea island Pellworm","volume":"57","author":"Posautz","year":"2015","journal-title":"Acta Vet Scand"},{"key":"ref30","first-page":"180","article-title":"Zwei sichere Altersmerkmale beim Hasen","volume":"47","author":"Stroh","year":"1931","journal-title":"Berl Tierarztl Wochenschr"},{"key":"ref31","volume-title":"Fauna d\u2019Italia: Acari Ixodida","author":"Manilla","year":"1986"},{"key":"ref32","volume-title":"Ticks of domestic Animals in Africa: a guide to Identification of Species","author":"Walker","year":"2003"},{"key":"ref33","volume-title":"Ticks of domestic Animals in the Mediterranean region: a guide to Identification of Species","author":"Estrada-Pe\u00f1a","year":"2004"},{"key":"ref34","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-63760-0","volume-title":"Ticks of Europe and North Africa: a guide to Species Identification","author":"Estrada-Pe\u00f1a","year":"2017"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1093\/jme\/tjab023","article-title":"Illustrated morphological keys for fleas (Siphonaptera) in Madagascar","volume":"58","author":"Harimalala","year":"2021","journal-title":"J Med Entomol"},{"key":"ref36","volume-title":"Fleas of public Health Importance and their Control","author":"Pratt","year":"1973"},{"key":"ref37","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511661754","volume-title":"The genus Rhipicephalus (Acari, Ixodidae): a guide to the brown Ticks of the World","author":"Walker","year":"2000"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1186\/s13071-017-2072-1","article-title":"Genetic characterizationv of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal","volume":"10","author":"Dantas-Torres","year":"2017","journal-title":"Parasit Vectors"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1186\/s13071-024-06572-3","article-title":"The Rhipicephalus sanguineus group: updated list of species, geographical distribution, and vector competence","volume":"17","author":"Dantas-Torres","year":"2024","journal-title":"Parasit Vectors"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1007\/s10493-021-00629-z","article-title":"Using MALDI-TOF mass spectrometry to identify ticks collected on domestic and wild animals from the Democratic Republic of the Congo","volume":"84","author":"Ngoy","year":"2021","journal-title":"Exp Appl Acarol"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"102632","DOI":"10.1016\/j.ttbdis.2026.102632","article-title":"Identification of ticks from north of Italy by MALDI-TOF MS","volume":"17","author":"Sampieri","year":"2026","journal-title":"Ticks Tick Borne Dis"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"5492","DOI":"10.1128\/jcm.41.12.5492-5499.2003","article-title":"Development of a multitarget real-time TaqMan PCR assay for enhanced detection of Francisella tularensis in complex specimens","volume":"41","author":"Versage","year":"2003","journal-title":"J Clin Microbiol"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/j.ttbdis.2012.10.019","article-title":"Rickettsiae of spotted fever group, Borrelia valaisiana, and Coxiella burnetii in ticks on passerine birds and mammals from the Camargue in the south of France","volume":"3","author":"Socolovschi","year":"2012","journal-title":"Ticks Tick Borne Dis"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1128\/jcm.42.1.90-98.2004","article-title":"Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of S\u00e3o Paulo, Brazil, where Brazilian spotted fever is endemic","volume":"42","author":"Labruna","year":"2004","journal-title":"J Clin Microbiol"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.mcp.2009.01.004","article-title":"Real-time PCR for the early detection and quantification of Coxiella burnetii as an alternative to the murine bioassay","volume":"23","author":"Howe","year":"2009","journal-title":"Mol Cell Probes"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"176","DOI":"10.3390\/pathogens9030176","article-title":"Upscaling the surveillance of tick-borne pathogens in the French Caribbean islands","volume":"9","author":"Gondard","year":"2020","journal-title":"Pathogens"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/s1386-6532(02)00168-3","article-title":"Development of a quantitative real-time RT-PCR assay with internal control for the laboratory detection of tick-borne encephalitis virus (TBEV) RNA","volume":"27","author":"Schwaiger","year":"2003","journal-title":"J Clin Virol"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.3201\/eid1307.070068","article-title":"Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients","volume":"13","author":"W\u00f6lfel","year":"2007","journal-title":"Emerg Infect Dis"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.jviromet.2013.02.019","article-title":"A novel quantitative multiplex real-time RT-PCR for the simultaneous detection and differentiation of West Nile virus lineages 1 and 2, and of Usutu virus","volume":"189","author":"Del Amo","year":"2013","journal-title":"J Virol Methods"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1177\/104063870601800505","article-title":"A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses","volume":"18","author":"Jim\u00e9nez-Clavero","year":"2006","journal-title":"J Vet Diagn Invest"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.jcv.2010.11.008","article-title":"A rapid and specific real-time RT-PCR assay to identify Usutu virus in human plasma, serum, and cerebrospinal fluid","volume":"50","author":"Cavrini","year":"2011","journal-title":"J Clin Virol"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"2082","DOI":"10.3390\/v15102082","article-title":"Development of a real-time PCR assay for the detection of the phlebovirus Fermo 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