{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:00:23Z","timestamp":1790334023524,"version":"4.1.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Vet. Sci."],"abstract":"<jats:p>\n                    Lipid metabolic dysregulation is increasingly recognized as a hallmark of cancer. Erythrocyte membrane fatty-acid (FA) profiling may reflect systemic metabolic alterations and provide non-invasive biomarkers of tumor biology. This prospective study investigated the erythrocyte membrane lipidomic profile of dogs with lymphoma and its potential prognostic relevance. Thirty-nine dogs with newly diagnosed lymphoma were enrolled. Clinical staging, flow-cytometric immunophenotyping, and erythrocyte membrane FA analysis were performed at diagnosis. Individual FAs, FA classes, and lipid-derived indices were compared between B-cell and T-cell lymphomas. Lymphoma-specific survival (LSS) was evaluated using Kaplan\u2013Meier analysis and exploratory univariate Cox proportional hazards models. Twenty-five dogs had B-cell lymphoma, and 14 had T-cell lymphoma. T-cell lymphomas were more frequently extranodal, whereas B-cell lymphomas were predominantly multicentric. Palmitoleic acid was significantly lower in T-cell than in B-cell lymphoma. Correlation analysis revealed distinct lipidomic interaction patterns in dogs with lymphoma compared with healthy controls, particularly involving polyunsaturated fatty acids, omega-6 fatty acids, arachidonic acid, and the unsaturation index.\n                    <jats:italic>The estimated LSS probability was approximately 50% at 180 days. Exploratory Cox analyses identified nominal associations between mixed diet and a lower hazard of lymphoma-related death, higher oleic acid concentrations and a lower hazard, and higher cis-vaccenic acid concentrations and a higher hazard<\/jats:italic>\n                    . These findings should be interpreted with caution given the limited sample size, multiple comparisons, and lack of multivariable adjustment. Overall, erythrocyte membrane FA profiling identified immunophenotype-related differences and exploratory associations with outcome, supporting further investigation of lipidomics as a potential prognostic tool in canine lymphoma.\n                  <\/jats:p>","DOI":"10.3389\/fvets.2026.1950771","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:07:56Z","timestamp":1790330876000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Erythrocyte membrane lipidomic signatures in canine lymphoma: immunophenotype-specific alterations and prognostic associations"],"prefix":"10.3389","volume":"13","author":[{"given":"Valentina","family":"Rinaldi","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Teramo","place":["Teramo, 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