{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:57:04Z","timestamp":1790827024274,"version":"4.1.0"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Transpl Int"],"abstract":"<jats:p>Non\u2010specific interferon\u2010gamma (IFN\u2010\u03b3) enzyme\u2010linked immunosorbent (ELISpot) responses after solid organ transplant (SOT) and their relationship with cytomegalovirus (CMV) reactivation have hardly been investigated. Adult kidney transplant (KT) recipients underwent measurement of IFN\u2010\u03b3\u2010producing T cells using the ELISpot assay before and 1 month after transplantation. Data for CMV infection episodes were collected. Risk factors for post\u2010transplant CMV infection, based on IFN\u2010\u03b3 responses, were analyzed using a Cox proportional hazards model. A total of 93 KT recipients were enrolled in the study and 84 evaluable participants remained at 1 month post KT. Thirty-three (39%) recipients developed subsequent CMV infection within 6 months post\u2010transplant. At 1\u2010month post\u2010transplant, IFN\u2010\u03b3\u2010producing T cells with &amp;lt;250 spot\u2010forming units (SFUs)\/2.5 \u00d7 10<jats:sup>5<\/jats:sup> peripheral blood mononuclear cells (PBMCs) were significantly associated with CMV infection (HR 3.1, 95% CI 1.4\u20137.1, <jats:italic>p<\/jats:italic> = 0.007). On multivariable analysis, posttransplant IFN\u2010\u03b3\u2010producing T cells with &amp;lt;250 SFUs\/2.5 \u00d7 10<jats:sup>5<\/jats:sup> PBMCs remained independently associated with CMV infection (HR 3.1, 95% CI 1.2\u20137.8, <jats:italic>p<\/jats:italic> = 0.019). Conclusions: KT recipients with low IFN\u2010\u03b3\u2010producing T cells measured by the ELISpot assay are more likely to develop CMV infection after transplantation. Therefore, measurement of nonspecific cell-mediated immunity ELISpot responses could potentially stratify recipients at risk of CMV infection (Thai Clinical Trials Registry, TCTR20210216004).<\/jats:p>","DOI":"10.3389\/ti.2023.11527","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T04:11:33Z","timestamp":1704427893000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Utility of the Interferon-Gamma Enzyme-Linked Immunosorbent Spot Assay to Predict Risk of Cytomegalovirus Infection in Kidney Transplant 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