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Secreted factors from mesenchymal stem cells (MSCs) that allow direct activation of preserved small intestine grafts. Freshly excised Luc-Tg LEW rat tissues were incubated in preservation solutions containing MSC-conditioned medium (MSC-CM). Preserved Luc-Tg rat-derived grafts were then transplanted to wild-type recipients, after which survival, injury score, and tight junction protein expression were examined. Luminance for each graft was determined using <jats:italic>in vivo<\/jats:italic> imaging. The findings indicated that 30\u2013100 and 3\u201310\u00a0kDa fractions of MSC-CM have superior activating effects for small intestine preservation. Expression of the tight-junction proteins claudin-3, and zonula occludens-1 preserved for 24\u00a0h in University of Wisconsin (UW) solution containing MSC-CM with 50\u2013100\u00a0kDa, as shown by immunostaining, also indicated effectiveness. Reflecting the improved graft preservation, MSC-CM preloading of grafts increased survival rate from 0% to 87%. This is the first report of successful transplantation of small intestine grafts preserved for more than 24\u00a0h using a rodent model to evaluate graft preservation conditions that mimic clinical conditions.<\/jats:p>","DOI":"10.3389\/ti.2024.11336","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T04:11:18Z","timestamp":1718770278000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Improved Preservation of Rat Small Intestine Transplantation Graft by Introduction of Mesenchymal Stem Cell-Secreted Fractions"],"prefix":"10.3389","volume":"37","author":[{"given":"Takumi","family":"Teratani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunaru","family":"Sakuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoya","family":"Kasahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Miki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan Kawarai","family":"Lefor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naohiro","family":"Sata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joji","family":"Kitayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1097\/TP.0000000000003065","article-title":"New Insights into the Indications for Intestinal Transplantation: Consensus in the Year 2019","volume":"104","author":"Kaufman","year":"2020","journal-title":"Transplantation"},{"key":"B2","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1034\/j.1399-0012.2000.140609.x","article-title":"Low Infectious Complications in Segmental Living Related Small Bowel Transplantation in Adults","volume":"14","author":"Cicalese","year":"2000","journal-title":"Clin Transpl"},{"key":"B3","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1097\/01.tp.0000149323.79759.5b","article-title":"Immunologic Benefits of Longer Graft in Rat Allogenic Small Bowel Transplantation","volume":"79","author":"Fujishiro","year":"2005","journal-title":"Transplantation"},{"key":"B4","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1111\/j.1432-2277.2010.01187.x","article-title":"Small Bowel Preservation for Intestinal Transplantation: A Review","volume":"24","author":"Roskott","year":"2011","journal-title":"Transpl Int"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1007\/s00595-010-4324-y","article-title":"Current Status of Intestinal Transplantation","volume":"40","author":"Ueno","year":"2010","journal-title":"Surg Today"},{"key":"B6","doi-asserted-by":"publisher","first-page":"5701","DOI":"10.3748\/wjg.v16.i45.5701","article-title":"Redefining the Properties of an Osmotic Agent in an Intestinal-Specific Preservation Solution","volume":"16","author":"Schlachter","year":"2010","journal-title":"World J Gastroenterol"},{"key":"B7","doi-asserted-by":"publisher","first-page":"S164","DOI":"10.1007\/s001470050452","article-title":"Long-Term Graft Survival after Liver Transplantation in the UW Era: Late Effects of Cold Ischemia and Primary Dysfunction. 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