{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:41:34Z","timestamp":1771663294871,"version":"3.50.1"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Transpl Int"],"abstract":"<jats:p>Cytomegalovirus (CMV) infection detrimentally influences graft survival in kidney transplant recipients, with the risk primarily determined by recipient and donor serostatus. However, recipient CD8<jats:sup>+<\/jats:sup> T cells play a crucial role in CMV control. The optimal preventive strategy (prophylaxis vs. pre-emptive treatment), particularly for seropositive (intermediate risk) recipients, remains uncertain. We investigated CD8<jats:sup>+<\/jats:sup> T cell subpopulation dynamics and CMV occurrence (DNAemia \u2265 100\u00a0IU\/mL) in 65 kidney transplant recipients, collecting peripheral blood mononuclear cells before (T1) and 1\u00a0year after transplantation (T2). Comparing the two timepoints, we found an increase in granulocyte, monocyte and CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells numbers, while FoxP3<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>, LAG-3<jats:sup>+<\/jats:sup> and PD-1<jats:sup>+<\/jats:sup> frequencies were reduced at T2. CMV DNAemia occurred in 33 recipients (55.8%) during the first year. Intermediate risk patients were disproportionally affected by posttransplant CMV (<jats:italic>N<\/jats:italic> = 29\/45, 64.4%). Intermediate risk recipients developing CMV after transplantation exhibited lower leukocyte, monocyte, and granulocyte counts and higher FoxP3<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup> frequencies in CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells pre-transplantation compared to patients staying CMV negative. Pre-transplant FoxP3<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup> in CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells had the best discriminatory potential for CMV infection prediction within the first year after transplantation (AUC: 0.746). The FoxP3<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup> CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cell subset may aid in selecting intermediate risk kidney transplant recipients for CMV prophylaxis.<\/jats:p>","DOI":"10.3389\/ti.2024.12963","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T10:28:15Z","timestamp":1716978495000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Pre-Transplant Frequencies of FoxP3+CD25+ in CD3+CD8+ T Cells as Potential Predictors for CMV in CMV-Intermediate Risk Kidney Transplant Recipients"],"prefix":"10.3389","volume":"37","author":[{"given":"Agnes 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