{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:47:39Z","timestamp":1792626459812,"version":"4.5.0"},"reference-count":17,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:00:00Z","timestamp":1720742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006319","name":"Centre National pour la Recherche Scientifique et Technique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Transpl Int"],"abstract":"<jats:p>The aim of this study was to provide insight into high-energy phosphate compound concentration dynamics under realistic clinical cold-storage conditions using the Celsior solution in seven heart grafts discarded from transplantation. The hearts of seven local donors (three males, four females, age 37 \u00b1 17\u00a0years, height 175 \u00b1 5\u00a0cm, weight 75 \u00b1 9\u00a0kg) initially considered for transplantation and eventually discarded were submitted to a Magnetic Resonance Spectroscopy observation in a clinical Magnetic Resonance Imaging scanner over at least 9\u00a0h. The grafts remained in their sterile container at 4\u00b0C during the entire examination. Hence, Phosphocreatine (PCr), adenosine triphosphate (ATP), inorganic phosphate (Pi) and intracellular pH were recorded non-destructively at a 30-minute interval. With the ischemic time Ti, the concentration ratios decreased at PCr\/ATP = 1.68\u22120.0028\u00b7Tis, Pi\/ATP = 1.38 + 0.0029\u00b7Tis, and intracellular pH at 7.43\u20130.0012\u00b7Tis. ATP concentration remained stable for at least 9\u00a0h and did not decrease as long as phosphocreatine was detectable. Acidosis remained moderate. In addition to the standard parameters assessed at the time of retrieval, Magnetic Resonance Spectroscopy can provide an assesment of the metabolic status of heart grafts before transplantation. These results show how HEPC metabolites deplete during cold storage. Although many parameters determine graft quality during cold storage, the dynamics of HEPC and intracellular pH may be helpful in the development of strategies aiming at extending the ischemic time.<\/jats:p>","DOI":"10.3389\/ti.2024.12994","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T04:11:15Z","timestamp":1720757475000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Time Course of High-Energy Phosphate Depletion During Cold Storage of Human Heart Grafts Using the Celsior Solution"],"prefix":"10.3389","volume":"37","author":[{"given":"Frank","family":"Kober","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Caus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Riberi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Le Fur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,7,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e018966","DOI":"10.1161\/JAHA.120.018966","article-title":"Cardiac Graft Assessment in the Era of Machine Perfusion: Current and Future Biomarkers","volume":"10","author":"Bona","year":"2021","journal-title":"J Am Heart Assoc"},{"key":"B2","doi-asserted-by":"publisher","first-page":"S938","DOI":"10.21037\/jtd.2019.02.59","article-title":"The Donor Heart and Organ Perfusion Technology","volume":"11","author":"Chew","year":"2019","journal-title":"J Thorac Dis"},{"key":"B3","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1097\/MAT.0b013e31823769d5","article-title":"Differences in Regional Myocardial Perfusion, Metabolism, MVO2, and Edema After Coronary Sinus Machine Perfusion Preservation of Canine Hearts","volume":"57","author":"Cobert","year":"2011","journal-title":"ASAIO J"},{"key":"B4","doi-asserted-by":"publisher","first-page":"2310","DOI":"10.1016\/j.jtcvs.2014.02.023","article-title":"Metabolic Characteristics of Human Hearts Preserved for 12 Hours by Static Storage, Antegrade Perfusion, or Retrograde Coronary Sinus Perfusion","volume":"148","author":"Cobert","year":"2014","journal-title":"J Thorac Cardiovasc Surg"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1752","DOI":"10.1097\/00007890-200212270-00018","article-title":"Objective and Noninvasive Metabolic Characterization of Donor Hearts by Phosphorous-31 Magnetic Resonance Spectroscopy","volume":"74","author":"Kober","year":"2002","journal-title":"Transplantation"},{"key":"B6","doi-asserted-by":"publisher","first-page":"2831","DOI":"10.1161\/01.cir.94.11.2831","article-title":"Functional Recovery After Human Heart Transplantation Is Related to the Metabolic Condition of the Hypothermic Donor Heart","volume":"94","author":"Van Dobbenburgh","year":"1996","journal-title":"Circulation"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1296","DOI":"10.1161\/01.CIR.67.6.1296","article-title":"Evaluation of High-Energy Phosphate Metabolism During Cardioplegic Arrest and Reperfusion: A Phosphorus-31 Nuclear Magnetic Resonance Study","volume":"67","author":"Pernot","year":"1983","journal-title":"Circulation"},{"key":"B8","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/s0022-5223(19)35297-3","article-title":"Three Solutions for Preservation of the Rabbit Heart at 0\u00b0 C","volume":"96","author":"English","year":"1988","journal-title":"J Thorac Cardiovasc Surg"},{"key":"B9","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/s0022-5223(19)34847-0","article-title":"Exogenous Adenosine Accelerates Recovery of Cardiac Function and Improves Coronary Flow After Long-Term Hypothermic Storage and Transplantation","volume":"104","author":"Gali\u00f1anes","year":"1992","journal-title":"J Thorac Cardiovasc Surg"},{"key":"B10","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/S0022-5223(19)38624-6","article-title":"Influence of the pH of Cardioplegic Solutions on Intracellular pH, High-Energy Phosphates, and Postarrest Performance. 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