{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T07:05:51Z","timestamp":1771484751794,"version":"3.50.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontierspartnerships.org"],"crossmark-restriction":true},"short-container-title":["Transpl. Int."],"abstract":"<jats:p>\n                    <jats:italic>Institut<\/jats:italic>\n                    <jats:italic>Georges Lopez-2M<\/jats:italic>\n                    (\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    ), a novel preservation solution containing polyethylene glycol (PEG 35kD, 5\u00a0g\/L), preserves mitochondrial integrity and redox balance in liver grafts. This study assesses\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    \u2019s effect on lung preservation during prolonged cold ischemia. Rat\u2019s heart-lung blocks were procured and subjected to 18\u00a0h cold ischemia (4\u00a0\u00b0C). Lungs were flushed and preserved using one of these preservation solutions:\n                    <jats:italic>OCS<\/jats:italic>\n                    ,\n                    <jats:italic>Perfadex Plus<\/jats:italic>\n                    ,\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    (n = 6\/group). Following ischemia, lungs underwent up to 7\u00a0h normothermic\n                    <jats:italic>ex vivo<\/jats:italic>\n                    lung perfusion. Edema was quantified by weight gain. Lung physiological parameters were recorded. Perfusate, bronchoalveolar lavage (BAL), and tissue samples were collected. All lungs in\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    group completed 7\u00a0h EVLP protocol. Compared to\n                    <jats:italic>OCS<\/jats:italic>\n                    ,\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    reduced edema formation (p &amp;lt; 0.01), preserved superior compliance (p &amp;lt; 0.01), and maintained lower pulmonary vascular resistance (p &amp;lt; 0.01).\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    showed lower perfusate concentrations of IL-1\u03b2 (p &amp;lt; 0.05), IL-6 (p &amp;lt; 0.05), and TNF-\u03b1 (p = 0.08). In BAL,\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    reduced IL-1\u03b2 (p &amp;lt; 0.01), IL-6 (p &amp;lt; 0.05), TNF-\u03b1 (p &amp;lt; 0.01), and CXCL1 (p &amp;lt; 0.01).\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    showed lower release of Syndecan-1 (p &amp;lt; 0.05). Compared to\n                    <jats:italic>Perfadex Plus<\/jats:italic>\n                    ,\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    was not inferior, with reduced expression of TNF-\u03b1 in the perfusate (p &amp;lt; 0.05).\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    effectively prevents edema development like\n                    <jats:italic>Perfadex Plus<\/jats:italic>\n                    .\n                    <jats:italic>IGL-2M<\/jats:italic>\n                    results in decreased inflammation and a stronger endothelial lining, making it a promising solution for lung preservation.\n                  <\/jats:p>","DOI":"10.3389\/ti.2026.15993","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T06:10:14Z","timestamp":1771481414000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Institut Georges Lopez-2M as a Novel Lung Preservation Solution Attenuates Ischemia-Reperfusion Injury in a Rat Ex Vivo Lung Perfusion Model"],"prefix":"10.3389","volume":"39","author":[{"given":"Annalisa","family":"Barbarossa","sequence":"first","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]},{"name":"Department of Thoracic Surgery, University Hospitals Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Van Slambrouck","sequence":"additional","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]},{"name":"Department of Thoracic Surgery, University Hospitals Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cara","family":"Trivett","sequence":"additional","affiliation":[{"name":"Centrum voor Moleculaire en Vasculaire Biologie, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ph\u00e9line","family":"Kortleven","sequence":"additional","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]},{"name":"Laboratory of Molecular Virology and Gene Therapy, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cedric","family":"Vanluyten","sequence":"additional","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]},{"name":"Department of Thoracic Surgery, University Hospitals Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto Martin","family":"Medina","sequence":"additional","affiliation":[{"name":"Centrum voor Moleculaire en Vasculaire Biologie, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Jin","sequence":"additional","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Jannis","sequence":"additional","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balin","family":"\u00d6zsoy","sequence":"additional","affiliation":[{"name":"Laboratory for Respiratory Diseases and Thoracic Surgery, Katholieke Universiteit Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Claes","sequence":"additional","affiliation":[{"name":"Department of 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