{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T17:19:48Z","timestamp":1775841588824,"version":"3.50.1"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell Dev. Biol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Peripheral nerve injuries, resulting from trauma or medical interventions, present significant clinical challenges due to their severe physiological and functional impacts. Despite various therapeutic approaches, optimal methods for promoting nerve regeneration remain difficult to obtain. This study is a preliminary step towards the future use of chitosan nerve guide conduits combined with human dental pulp stem cells and their conditioned media to promote nerve regrowth in a rat model with severe sciatic nerve damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Preliminary characterization of conditioned medium from human dental pulp stem cells identified key regenerative biomarkers using a Multiplexing LASER Bead analysis. The human dental pulp stem cells\u2019 cytocompatibility with Reaxon\u00ae chitosan biomaterial was confirmed through viability and metabolic assays in a PrestoBlue assay, along with scanning electron microscopy and energy-dispersive X-ray spectroscopy analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>These <jats:italic>in vitro<\/jats:italic> assessments validated the therapeutic potential of the combinations for nerve regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Future <jats:italic>in vivo<\/jats:italic> experiments will involve applying these combinations in a rat model, with functional assessments to evaluate efficacy. This research aims to establish human dental pulp stem cells and nerve guide conduits as viable treatments for peripheral nerve injury, offering promising directions for clinical applications.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcell.2025.1528213","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T06:53:43Z","timestamp":1739948023000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["In vitro evaluation of dental pulp stem cells for sciatic nerve regeneration: foundations for future in vivo applications"],"prefix":"10.3389","volume":"13","author":[{"given":"Bruna","family":"Lopes","sequence":"first","affiliation":[]},{"given":"Ana Catarina","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Patr\u00edcia","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Al\u00edcia","family":"de Sousa Moreira","sequence":"additional","affiliation":[]},{"given":"Andr\u00e9 Filipe","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds","family":"Atayde","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio J.","family":"Salgado","sequence":"additional","affiliation":[]},{"given":"Stefano","family":"Geuna","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Alvites","sequence":"additional","affiliation":[]},{"given":"Ana Colette","family":"Maur\u00edcio","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.3390\/pharmaceutics13101686","article-title":"Chitosan nanoparticles at the biological interface: implications for drug delivery","volume":"13","author":"Aibani","year":"2021","journal-title":"Pharmaceutics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1466404","DOI":"10.1080\/2331205x.2018.1466404","article-title":"Peripheral nerve injury and axonotmesis: state of the art and recent advances","volume":"5","author":"Alvites","year":"2018","journal-title":"Cogent Med."},{"key":"B3","doi-asserted-by":"publisher","first-page":"6613029","DOI":"10.1155\/2021\/6613029","article-title":"Combined use of chitosan and olfactory mucosa mesenchymal stem\/stromal cells to promote peripheral nerve regeneration in vivo","volume":"2021","author":"Alvites","year":"2021","journal-title":"Stem Cells Int."},{"key":"B4","doi-asserted-by":"publisher","first-page":"8034","DOI":"10.1016\/j.biomaterials.2012.07.056","article-title":"A systematic review of animal models used to study nerve regeneration in tissue-engineered scaffolds","volume":"33","author":"Angius","year":"2012","journal-title":"Biomaterials"},{"key":"B5","doi-asserted-by":"publisher","first-page":"2479","DOI":"10.3390\/nano13172479","article-title":"Human dental pulp mesenchymal stem cell-derived soluble factors combined with a nanostructured scaffold support the generation of a vascular network in vivo","volume":"13","author":"Barone","year":"2023","journal-title":"Nanomater. 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