{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:46:23Z","timestamp":1753890383684,"version":"3.41.2"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:00:00Z","timestamp":1716336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2022.01152.PTDC UIDP\/04378\/2020 UIDB\/04378\/2020 LA\/P\/0140\/2020 UIDB\/50006\/2020 UIDP\/50006\/2020 LA\/P\/0008\/2020"],"award-info":[{"award-number":["2022.01152.PTDC UIDP\/04378\/2020 UIDB\/04378\/2020 LA\/P\/0140\/2020 UIDB\/50006\/2020 UIDP\/50006\/2020 LA\/P\/0008\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Chem. Biol."],"abstract":"<jats:p>Cytochrome <jats:italic>c<\/jats:italic><jats:sub>552<\/jats:sub> from <jats:italic>Wolinella succinogenes<\/jats:italic> is one of the few examples of a low reduction potential class I <jats:italic>c<\/jats:italic>-type cytochrome with a mixture of high\/low spin state populations observed in its visible spectrum. Analysis of its structural model suggests that the heme is Met\/His coordinated and highly solvent-exposed. This supports the hypothesis that it is the solvent accessibility of the propionate groups that controls the reduction potential of this small <jats:italic>c<\/jats:italic>-type cytochrome. The visible spectra obtained at different pH values reveal the presence of a protonable group with a p<jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub> of 7.3, which also influences the reduction potential of this small cytochrome <jats:italic>c<\/jats:italic><jats:sub>552<\/jats:sub> (E<jats:sub>m<\/jats:sub><jats:sup>0\u2019<\/jats:sup> of 97 \u00b1 5\u00a0mV, pH 7.0) and can be either an H<jats:sub>2<\/jats:sub>O\/OH<jats:sup>\u2212<\/jats:sup> group distantly coordinating the heme iron, or one of the propionate groups. The thermostability of cytochrome <jats:italic>c<\/jats:italic><jats:sub>552<\/jats:sub> was studied by circular dichroism and differential scanning calorimetry, indicating a highly stable protein at pH 5\u20137 (90\u00b0C to 77\u00b0C).<\/jats:p>","DOI":"10.3389\/fchbi.2024.1398105","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T04:49:12Z","timestamp":1716353352000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of pH on the thermostability and redox properties of cytochrome c552 from Wolinella succinogenes"],"prefix":"10.3389","volume":"3","author":[{"given":"Vitor H.","family":"Mordido","sequence":"first","affiliation":[]},{"given":"Marta S. 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