{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:20:36Z","timestamp":1787768436647,"version":"build-2784847793"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T00:00:00Z","timestamp":1701216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Fungal Biol."],"abstract":"<jats:p><jats:italic>Psilocybe<\/jats:italic> mushrooms, otherwise known as \u201cmagic\u201d mushrooms, owe their psychedelic effect to psilocin, a serotonin subtype 2A (5-HT<jats:sub>2A<\/jats:sub>) receptor agonist and metabolite of psilocybin, the primary indole alkaloid found in <jats:italic>Psilocybe<\/jats:italic> species. Metabolomics is an advanced fingerprinting tool that can be utilized to identify the differences among fungal life stages that may otherwise be unaccounted for. In this study, by using targeted and untargeted (metabolomic) multivariate analysis, we demonstrate that the chemical composition of <jats:italic>Psilocybe<\/jats:italic> differs among mycelia, grain mycelia, and fruiting bodies. The preferential accumulation of psilocybin, baeocystin, tryptophan, ergothioneine, and phenylethylamine in fruiting bodies differentiated them from mycelia; however, the levels of alpha-glycerylphosphorylcholine (\u03b1-GPC), <jats:italic>N-<\/jats:italic>acetylglucosamine, and trimethylglycine were found to be proportionally higher in mycelia than in fruiting bodies based on Pareto-scaled data. Considering the wealth of compounds with therapeutic potential that have been isolated from various fungal genera, it would be pertinent to study the compounds found in <jats:italic>Psilocybe<\/jats:italic> mycelia as potential naturally derived therapeutic targets.<\/jats:p>","DOI":"10.3389\/ffunb.2023.1295223","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T05:02:44Z","timestamp":1701234164000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Exploring Psilocybe spp. mycelium and fruiting body chemistry for potential therapeutic compounds"],"prefix":"10.3389","volume":"4","author":[{"given":"Adam","family":"Waldbillig","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Baranova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharan","family":"Sidhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,11,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1016\/j.neuropharm.2011.05.023","article-title":"Functional crosstalk and heteromerization of serotonin 5-HT2A and dopamine D2 receptors","volume":"61","author":"Albizu","year":"2011","journal-title":"Neuropharmacology"},{"key":"B2","doi-asserted-by":"publisher","first-page":"14672","DOI":"10.1074\/jbc.271.25.14672","article-title":"Mapping the binding site pocket of the serotonin 5-hydroxytryptamine2A receptor: Ser3. 36 (159) provides a second interaction site for the protonated amine of serotonin but not of lysergic acid diethylamide or bufotenin","volume":"271","author":"Almaula","year":"1996","journal-title":"J. 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