{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T16:05:29Z","timestamp":1777910729197,"version":"3.51.4"},"reference-count":70,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T00:00:00Z","timestamp":1678147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Grapes\u2019 infection by phytopathogenic fungi may often lead to rot and impair the quality and safety of the final product. Due to the concerns associated with the extensive use of chemicals to control these fungi, including their toxicity for environment and human health, bio-based products are being highly preferred, as eco-friendlier and safer alternatives. Specifically, yeasts have shown to possess antagonistic activity against fungi, being promising for the formulation of new biocontrol products.In this work 397 wine yeasts, isolated from Portuguese wine regions, were studied for their biocontrol potential against common grapes phytopathogenic fungal genera:\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    ,\n                    <jats:italic>Botrytis<\/jats:italic>\n                    ,\n                    <jats:italic>Rhizopus<\/jats:italic>\n                    and\n                    <jats:italic>Penicillium<\/jats:italic>\n                    . This set comprised strains affiliated to 32 species distributed among 20 genera. Time-course monitoring of mold growth was performed to assess the inhibitory activity resulting from either diffusible or volatile compounds produced by each yeast strain. All yeasts displayed antagonistic activity against at least one of the mold targets.\n                    <jats:italic>Rhizopus<\/jats:italic>\n                    was the most affected being strongly inhibited by 68% of the tested strains, followed by\n                    <jats:italic>Botrytis<\/jats:italic>\n                    (20%),\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    (19%) and\n                    <jats:italic>Penicillium<\/jats:italic>\n                    (7%). More notably, the approach used allowed the detection of a wide array of yeast-induced mold response profiles encompassing, besides the decrease of mold growth, the inhibition or delay of spore germination and the complete arrest of mycelial extension, and even its stimulation at different phases. Each factor considered (taxonomic affiliation, mode of action and fungal target) as well as their interactions significantly affected the antagonistic activity of the yeast isolates. The highest inhibitions were mediated by volatile compounds. Total inhibition of\n                    <jats:italic>Penicillium<\/jats:italic>\n                    was achieved by a strain of\n                    <jats:italic>Metschnikowia pulcherrima<\/jats:italic>\n                    , while the best performing yeasts against\n                    <jats:italic>Rhizopus<\/jats:italic>\n                    ,\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    and\n                    <jats:italic>Botrytis<\/jats:italic>\n                    , belong to\n                    <jats:italic>Lachancea thermotolerans<\/jats:italic>\n                    ,\n                    <jats:italic>Hanseniaspora uvarum<\/jats:italic>\n                    and\n                    <jats:italic>Starmerella bacillaris<\/jats:italic>\n                    , respectively. Notwithstanding the wide diversity of yeasts tested, only three strains were found to possess a broad spectrum of antagonistic activity, displaying strong or very strong inhibition against the four fungal targets tested. Our results confirm the potential of wine yeasts as biocontrol agents, while highlighting the need for the establishment of fit-for-purpose selection programs depending on the mold target, the timing, and the mode of application.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2023.1146065","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T01:00:47Z","timestamp":1678150847000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":27,"title":["Biocontrol potential of wine yeasts against four grape phytopathogenic fungi disclosed by time-course monitoring of inhibitory activities"],"prefix":"10.3389","volume":"14","author":[{"given":"Marcos","family":"Esteves","sequence":"first","affiliation":[]},{"given":"Patr\u00edcia","family":"Lage","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Filipe","family":"Centeno","sequence":"additional","affiliation":[]},{"given":"Maria","family":"de F\u00e1tima Teixeira","sequence":"additional","affiliation":[]},{"given":"Rog\u00e9rio","family":"Tenreiro","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Mendes-Ferreira","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2023,3,7]]},"reference":[{"key":"ref1","first-page":"169","article-title":"Isolation, screening and identification of mannanase producing microorganisms","volume":"1","author":"Asfamawi","year":"2013","journal-title":"J. Trop. Agric. Fd. Sci."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.ijfoodmicro.2011.11.025","article-title":"The microbial ecology of wine grape berries","volume":"153","author":"Barata","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"8","DOI":"10.3390\/fermentation4010008","article-title":"Molecular and phenotypic characterization of Metschnikowia pulcherrima strains from Douro wine region","volume":"4","author":"Barbosa","year":"2018","journal-title":"Fermentation"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1080\/07060661.2012.726649","article-title":"Mode of action of biocontrol agents: all that glitters is not gold","volume":"34","author":"B\u00e9langer","year":"2012","journal-title":"Can. J. Plant Pathol."},{"key":"ref5","first-page":"373","article-title":"Fungal and bacterial volatile organic compounds: an overview and their role as ecological signaling agents","volume-title":"The Mycota IX Fungal Interactions","author":"Bennett","year":"2012"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1094\/MPMI-10-12-0249-CR","article-title":"Sniffing on microbes: diverse roles of microbial volatile organic compounds in plant health","volume":"26","author":"Bitas","year":"2013","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.ijfoodmicro.2005.12.004","article-title":"Isolation of Epiphytic yeasts with potential for biocontrol of Aspergillus carbonarius and Aspergillus niger on grape","volume":"108","author":"Bleve","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"E139","DOI":"10.1073\/pnas.1317377110","article-title":"Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate","volume":"111","author":"Bokulich","year":"2014","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3390\/fermentation4040085","article-title":"The multiple and versatile roles of Aureobasidium pullulans in the vitivinicultural sector","volume":"4","author":"Bozoudi","year":"2018","journal-title":"Fermentation"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1007\/BF02876772","article-title":"The effect of volatile and gaseous metabolites of swelling seeds on germination of fungal spores","volume":"20","author":"Catskx","year":"1975","journal-title":"Folia Microbiol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1080\/12298093.2018.1454013","article-title":"Screening and evaluation of yeast antagonists for biological control of Botrytis cinerea on strawberry fruits","volume":"46","author":"Chen","year":"2018","journal-title":"Mycobiology"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2017.02025","article-title":"Wild grape-associated yeasts as promising biocontrol agents against Vitis vinifera fungal pathogens","volume":"8","author":"Cordero-Bueso","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s43008-019-0009-3","article-title":"Analysis of spatio-temporal fungal growth dynamics under different environmental conditions","volume":"10","author":"De Ligne","year":"2019","journal-title":"IMA Fungus"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/foods10071650","article-title":"The role of yeasts as biocontrol agents for pathogenic fungi on postharvest grapes: a review","volume":"10","author":"Di Canito","year":"2021","journal-title":"Foods"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.biocontrol.2014.10.004","article-title":"Production of volatile organic compounds by Aureobasidium pullulans as a potential mechanism of action against postharvest fruit pathogens","volume":"81","author":"Di Francesco","year":"2015","journal-title":"Biol. Control"},{"key":"ref21","first-page":"1","year":"2016"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2018.06.023","article-title":"Effect of yeast volatile organic compounds on ochratoxin A-producing Aspergillus carbonarius and Aspergillus ochraceus","volume":"284","author":"Farbo","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"104683","DOI":"10.1016\/j.biocontrol.2021.104683","article-title":"Successful biocontrol of major postharvest and soil-borne plant Pathogenic fungi by Antagonistic yeasts","volume":"160","author":"Fernandez-San Millan","year":"2021","journal-title":"Biol. 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Biotechnol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/s12550-014-0189-z","article-title":"The major volatile compound 2-phenylethanol from the biocontrol yeast, Pichia anomala, inhibits growth and expression of aflatoxin biosynthetic genes of Aspergillus flavus","volume":"30","author":"Hua","year":"2014","journal-title":"Mycotoxin Res."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/J.BIOCONTROL.2012.02.010","article-title":"Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-harvest strawberry fruits","volume":"62","author":"Huang","year":"2012","journal-title":"Biol. Control"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1094\/PHYTO-09-10-0255","article-title":"Control of postharvest botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia","volume":"101","author":"Huang","year":"2011","journal-title":"Phytopathology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-3-319-60021-5_4","article-title":"Fungi of grapes","volume-title":"Biology of Microorganisms on Grapes, in Must and in Wine","author":"Kassemeyer","year":"2017"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fpls.2019.00845","article-title":"Mode of action of microbial biological control agents against plant diseases: relevance beyond efficacy","volume":"10","author":"K\u00f6hl","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1038\/nrmicro2580","article-title":"The social network: deciphering fungal language","volume":"9","author":"Leeder","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2016.01499","article-title":"Biocontrol ability and action mechanism of Starmerella bacillaris (synonym Candida zemplinina) isolated from wine musts against gray mold disease agent Botrytis cinerea on grape and their effects on alcoholic fermentation","volume":"7","author":"Lemos Junior","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1021\/acs.jafc.1c07302","article-title":"Use of non-saccharomyces yeasts in berry wine production: inspiration from their applications in winemaking","volume":"70","author":"Liu","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2022.986229","article-title":"Antifungal activity of non-conventional yeasts against Botrytis cinerea and non-botrytis grape bunch rot fungi","volume":"13","author":"Maluleke","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1002\/yea.1304","article-title":"Influence of volatile compounds produced by yeasts predominant during processing of Coffea arabica in East Africa on growth and ochratoxin a (OTA) production by Aspergillus ochraceus","volume":"22","author":"Masoud","year":"2005","journal-title":"Yeast"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.postharvbio.2011.09.009","article-title":"Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina","volume":"64","author":"Nally","year":"2012","journal-title":"Postharvest Biol. Technol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.postharvbio.2013.07.022","article-title":"Biocontrol of fungi isolated from sour rot infected table grapes by Saccharomyces and other yeast species","volume":"86","author":"Nally","year":"2013","journal-title":"Postharvest Biol. Technol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"2765","DOI":"10.1128\/AEM.01864-06","article-title":"Yeast populations residing on healthy or botrytis-infected grapes from a vineyard in Attica, Greece","volume":"73","author":"Nisiotou","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref36","year":"2022"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.ijfoodmicro.2017.10.027","article-title":"Volatile organic compounds from Wickerhamomyces anomalus, Metschnikowia pulcherrima and Saccharomyces cerevisiae inhibit growth of decay causing fungi and control postharvest diseases of strawberries","volume":"265","author":"Oro","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"577","DOI":"10.3390\/horticulturae8070577","article-title":"Advances and perspectives in the use of biocontrol agents against fungal plant diseases","volume":"8","author":"Palmieri","year":"2022","journal-title":"Horticulturae"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.biocontrol.2015.04.021","article-title":"Isolation, identification and in vitro screening of grapevine yeasts for the control of black aspergilli on grapes","volume":"88","author":"Pantelides","year":"2015","journal-title":"Biol. Control"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.fm.2014.11.013","article-title":"Biocontrol ability and action mechanism of food-isolated yeast strains against Botrytis cinerea causing post-harvest bunch rot of table grape","volume":"47","author":"Parafati","year":"2015","journal-title":"Food Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"166","DOI":"10.3390\/jof7030166","article-title":"Screening methods for isolation of biocontrol Epiphytic yeasts against Penicillium digitatum in lemons","volume":"7","author":"Pereyra","year":"2021","journal-title":"J. Fungi"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.cropro.2016.11.025","article-title":"A critical review of plant protection tools for reducing pesticide use on grapevine and new perspectives for the implementation of IPM in viticulture","volume":"97","author":"Pertot","year":"","journal-title":"Crop Prot."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.cropro.2017.01.010","article-title":"Combining biocontrol agents with different mechanisms of action in a strategy to control Botrytis cinerea on grapevine","volume":"97","author":"Pertot","year":"","journal-title":"Crop Prot."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2017.00995","article-title":"Microbial resources and enological significance: opportunities and benefits","volume":"8","author":"Petruzzi","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/0022-5193(66)90013-0","article-title":"The measurement of diversity in different types of biological collections","volume":"13","author":"Pielou","year":"1966","journal-title":"J. Theor. Biol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2015.00905","article-title":"Wine fermentation microbiome: a landscape from different Portuguese wine appellations","volume":"6","author":"Pinto","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1080\/09583157.2019.1687645","article-title":"Foliar application of endophytic Wickerhamomyces anomalus against grey mould in Eucalyptus dunnii","volume":"30","author":"Poitevin","year":"2020","journal-title":"Biocontrol Sci. Tech."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3390\/fermentation4020031","article-title":"Yeasts from different habitats and their potential as biocontrol agents","volume":"4","author":"Pretscher","year":"2018","journal-title":"Fermentation"},{"key":"ref50","first-page":"336","article-title":"Biocontrol of grey mould disease on grape caused by Botrytis cinerea with autochthonous wine yeasts","volume":"48","author":"Raspor","year":"2010","journal-title":"Food Technol. Biotechnol."},{"key":"ref51","doi-asserted-by":"publisher","DOI":"10.20944\/preprints201909.0113.v1","article-title":"Evaluation of native wine yeast as biocontrol agents against fungal pathogens related to postharvest diseases","author":"Reyes-bravo","year":"2019","journal-title":"Agric. Sci. Agron."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.fm.2009.12.009","article-title":"Yeast biodiversity from oleic ecosystems: study of their biotechnological properties","volume":"27","author":"Romo-S\u00e1nchez","year":"2010","journal-title":"Food Microbiol."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1007\/s11095-010-0363-8","article-title":"Assessment of pharmacologic area under the curve when baselines are variable","volume":"28","author":"Scheff","year":"2011","journal-title":"Pharm. Res."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1093\/dnares\/dsy039","article-title":"Genome sequence of the non-conventional wine yeast Hanseniaspora guilliermondii UTAD222 unveils relevant traits of this species and of the Hanseniaspora genus in the context of wine fermentation","volume":"26","author":"Seixas","year":"2019","journal-title":"DNA Res."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.resmic.2004.12.005","article-title":"Mycotoxin-producing and other fungi isolated from grapes for wine production, with particular emphasis on ochratoxin a","volume":"156","author":"Serra","year":"2005","journal-title":"Res. Microbiol."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"6716","DOI":"10.1128\/AEM.01275-06","article-title":"Metschnikowia strains isolated from botrytized grapes antagonize fungal and bacterial growth by iron depletion","volume":"72","author":"Sipiczki","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"107","DOI":"10.3390\/taxonomy2010009","article-title":"Taxonomic revision of the pulcherrima clade of Metschnikowia (fungi): merger of species","volume":"2","author":"Sipiczki","year":"2022","journal-title":"Taxon"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2019.00697","article-title":"Taxonomic and functional characterization of the microbial community during spontaneous in vitro fermentation of Riesling must","volume":"10","author":"Sir\u00e9n","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1016\/j.tifs.2021.08.037","article-title":"Molecular explication of grape berry-fungal infections and their potential application in recent postharvest infection control strategies","volume":"116","author":"Solairaj","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"9803","DOI":"10.3390\/ijms14059803","article-title":"Two volatile organic compounds trigger plant self-defense against a bacterial pathogen and a sucking insect in cucumber under open field conditions","volume":"14","author":"Song","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.tifs.2015.11.003","article-title":"Development of biocontrol products for postharvest diseases of fruit: the importance of elucidating the mechanisms of action of yeast antagonists","volume":"47","author":"Spadaro","year":"2016","journal-title":"Trends Food Sci. Technol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1007\/s10340-021-01354-7","article-title":"When is it biological control? A framework of definitions, mechanisms, and classifications","volume":"94","author":"Stenberg","year":"2021","journal-title":"J. Pest. Sci."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1046\/j.1365-2672.2001.01379.x","article-title":"Screening for the production of extracellular hydrolytic enzymes by non-saccharomyces wine yeasts","volume":"91","author":"Strauss","year":"2001","journal-title":"J. Appl. Microbiol."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1111\/j.1365-2672.1995.tb05030.x","article-title":"Natural wine yeasts as biocontrol agents","volume":"78","author":"Suzzi","year":"1995","journal-title":"J. Appl. Bacteriol."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1080\/09583159650039584","article-title":"Isolation of microbial antagonists for biocontrol of grey mould disease of strawberries","volume":"6","author":"Swadling","year":"1996","journal-title":"Biocontrol Sci. Tech."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"41","DOI":"10.3389\/fmicb.2020.00041","article-title":"Scent of a killer: microbial Volatilome and its role in the biological control of plant pathogens","volume":"11","author":"Tilocca","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"2878","DOI":"10.3390\/foods10112878","article-title":"An integrative view of the role of Lachancea thermotolerans in wine technology","volume":"10","author":"Vicente","year":"2021","journal-title":"Foods"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1111\/epp.12307","article-title":"The regulatory landscape for biological control agents","volume":"46","author":"Ward","year":"2016","journal-title":"Bull. OEPP\/EPPO"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1146\/annurev.py.27.090189.002233","article-title":"Biological control of post harvest diseases offruit and vegetables: an emerging technology","volume":"27","author":"Wilson","year":"1989","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref70","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1145\/1978942.1978963","article-title":"The aligned rank transform for nonparametric factorial analyses using only ANOVA procedures","volume-title":"Proceedings of the SIGCHI Conference on Human Factors in Computing Systems","author":"Wobbrock","year":"2011"},{"key":"ref71","volume-title":"Biostatistical Analysis","author":"Zar","year":"1996"},{"key":"ref72","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1080\/1040841X.2020.1794793","article-title":"Biological control as an alternative to synthetic fungicides for the management of grey and blue mould diseases of table grapes: a review","volume":"46","author":"Zhang","year":"2020","journal-title":"Crit. Rev. 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