{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:06:36Z","timestamp":1761897996380,"version":"3.41.2"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Yttrium (Y), an important rare earth element (REE), is increasingly prevalent in the environment due to industrial activities, raising concerns about its toxicity. Understanding the effects of Y on microorganisms is essential for bioremediation and biorecovery processes. This study investigates how <jats:italic>Mesorhizobium qingshengii<\/jats:italic> J19, a strain with notable resistance to Y, manages iron homeostasis as a detoxifying mechanism under Y stress. Using comparative genomic and transcriptomic analyses, we explored the gene expression profile of strain J19 to identify the mechanisms underlying its high Y resistance and effective Y removal from the medium. Genome-wide transcriptional profiling revealed 127 significantly differentially expressed genes out of 6,343 under Y stress, with 36.2 % up-regulated and 63.8 % down-regulated. Notably, Y exposure significantly affects cellular iron homeostasis and activates arsenic detoxifying mechanisms. A key finding was the 7.6-fold up-regulation of a TonB transporter gene, indicating its crucial role in Y detoxification. Real-time PCR (RT-PCR) analysis of the selected gene confirmed the accuracy of RNA sequencing results. Further validation showed that iron supplementation mitigates Y-induced growth inhibition, leading to reduced ROS production in strain J19. This study elucidates the molecular mechanisms by which strain <jats:italic>M. qingshengii<\/jats:italic> J19 adapts to Y stress, emphasizing the importance of iron in controlling ROS and protecting against Y toxicity. It also highlights critical pathways and adaptive responses involved in the strain\u2019s resilience to metal stress.<\/jats:p>","DOI":"10.3389\/fmicb.2024.1467386","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T04:46:27Z","timestamp":1728017187000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Iron homeostasis as a cell detoxification mechanism in Mesorhizobium qingshengii J19 under yttrium exposure"],"prefix":"10.3389","volume":"15","author":[{"given":"Carina","family":"Coimbra","sequence":"first","affiliation":[]},{"given":"Paula V.","family":"Morais","sequence":"additional","affiliation":[]},{"given":"Rita","family":"Branco","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9081628","article-title":"Unraveling the underlying heavy metal detoxification mechanisms of Bacillus species.","volume":"9","author":"Alotaibi","year":"2021","journal-title":"Microorganisms"},{"key":"B2","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/0269-7491(93)90159-L","article-title":"Indium detoxification in Pseudomonas fluorescens.","volume":"82","author":"Anderson","year":"1993","journal-title":"Environ. 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