{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:10:46Z","timestamp":1768968646629,"version":"3.49.0"},"reference-count":134,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Classical methods for <jats:italic>postmortem<\/jats:italic> interval (PMI) estimation have been applied for nearly a century. Contrary to the notion of being simple or easily accessible, these approaches require highly specialized training, including a medical degree, postgraduate specialization in forensic pathology, and extensive practical experience. Classical PMI estimation relies on observable physical and chemical changes in the human cadaver, such as <jats:italic>rigor mortis, livor mortis, algor mortis<\/jats:italic>, and transformative processes during decomposition. These methods are fundamental in medicolegal practice but remain largely influenced by environmental and individual variability. Recent advances in forensic research, particularly in microbiology and biochemistry, have introduced innovative approaches that complement traditional methods, offering greater accuracy and reliability, though resource-intensive. Emerging approaches leverage the predictable <jats:italic>postmortem<\/jats:italic> succession of microbial communities (thanatomicrobiome) and biochemical alterations in cadaver fluids and tissues. Techniques such as metagenomics, metatranscriptomics, and metabolomics enable detailed analysis of these changes, while computational models and machine learning further refine PMI estimates. Despite advancements, challenges persist, including variability due to environmental factors and limited access to human decomposition data. Integrating multi-omics approaches and artificial intelligence offers a path forward, addressing these limitations and enhancing the accuracy of PMI estimation. This review provides a comprehensive overview of PMI estimation, critically examining classical approaches and highlighting cutting-edge methodologies rooted in thanatomicrobiology and thanatochemistry. We emphasize the transformative potential of multi-omics integration and artificial intelligence in improving PMI accuracy. Importantly, we propose a paradigm shift: redefining PMI estimation through evidence-based, interdisciplinary research that bridges scientific rigor and judicial application. Transdisciplinary collaboration and standardized methodologies will be essential to translate emerging knowledge into robust forensic tools that serve both science and justice.<\/jats:p>","DOI":"10.3389\/fmicb.2025.1646907","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T05:29:28Z","timestamp":1760678968000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Redefining postmortem interval estimation: the need for evidence-based research to bridge science and justice"],"prefix":"10.3389","volume":"16","author":[{"given":"Maria J.","family":"Teixeira","sequence":"first","affiliation":[]},{"given":"Daniel J.","family":"Barbosa","sequence":"additional","affiliation":[]},{"given":"Ricardo Jorge","family":"Dinis-Oliveira","sequence":"additional","affiliation":[]},{"given":"Ana R.","family":"Freitas","sequence":"additional","affiliation":[]}],"member":"1965","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.forsciint.2017.07.017","article-title":"Daily thanatomicrobiome changes in soil as an approach of postmortem interval estimation: an ecological perspective","volume":"278","author":"Adserias-Garriga","year":"2017","journal-title":"Forensic Sci. 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